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NKX3.1 HD amide chemical shift perturbations due to AD interactions. (A) NKX3.1 (114–184) with 0.8 mM phosphorylated AD peptide, with the y-axis on the left. (B) NKX3.1 (114–184) with 1.6 mM nonphosphorylated AD peptide, with the y-axis on the right. (C) NKX3.1 (114–184) plus 1.6 mM ADSI peptide, with the y-axis on the right. (D) HD perturbations in NKX3.1 (75–184) versus (114–184), with the y-axis on the left.
