CELL BIOLOGY Correction for “Structural basis of yeast Tim40/Mia40 as an oxidative translocator in the mitochondrial intermembrane space,” by Shin Kawano, Koji Yamano, Mari Naoé, Takaki Momose, Kayoko Terao, Shuh-ichi Nishikawa, Nobuhisa Watanabe, and Toshiya Endo, which appeared in issue 34, August 25, 2009, of Proc Natl Acad Sci USA (106:14403–14407; first published August 10, 2009; 10.1073/pnas.0901793106).
The authors note that on page 14406, Fig. 4D was mislabeled. The words “oxidized” and “reduced” should have been reversed in both the upper and lower panels. These errors do not affect the conclusions of the article. The corrected figure and its legend appear below.
Fig. 4.
Effects of Mia40 Phe mutations on cell growth and protein import. (A) Δtim40/pRS316-Tim40 harboring the pRS314 vector alone (vector) or plasmid expressing WT or a series of Phe→Glu mutants of the MIA40 gene from its own promoter were grown on a SCD plate lacking tryptophan and uracil (−Trp −Ura) or a SCD plate lacking tryptophan, but containing 0.1% 5-fluoroorotic acid (−Trp FOA) at 23 °C for 3 days. (B) Cells of Δtim40/pRS314-Tim40 (WT), Δtim40/pRS314-Tim40 (F311E), and Δtim40/pRS314-Tim40 (F334E) harboring vector (pYO326) alone or pYO326-ERV1 (ERV1) were grown to an early stationary phase in SCD lacking tryptophan and uracil at 23 °C. Cells were diluted in 10-fold increments, and 10 μL of each dilution (starting from 100-fold dilution) was spotted onto SCD lacking tryptophan and uracil and incubated at 23 °C for 3 days or at 37 °C for 2 days. (C) Mitochondria were isolated from the WT strain harboring pYO326 vector, F311E mutant strains harboring pYO326 vector (F311E), or pYO326-ERV1 (F311E, Erv1↑), which were grown in lactate medium at 23 °C. Radiolabeled Tim9 was incubated with the indicated mitochondria at 32 °C for indicated times in the presence of 50 μM ZnSO4. The reisolated mitochondria were solubilized with 1% digitonin and analyzed by BN-PAGE followed by radioimaging. An asterisk indicates nonspecific bands. (D) Proteins of indicated mitochondria (4.2 and 50 μg for each mitochondrial preparation) were analyzed by reducing (Left, with β-mercaptoethanol) or nonreducing (Right, without β-mercaptoethanol) SDS/PAGE followed by immunoblotting with anti-Mia40 (Upper) and anti-Erv1 (Lower) antibodies. The oxidized and reduced forms of Mia40, the Erv1 homodimer, and the Mia40-Erv1 complex are indicated.