Skip to main content
Journal of Bacteriology logoLink to Journal of Bacteriology
. 1963 Nov;86(5):1030–1036. doi: 10.1128/jb.86.5.1030-1036.1963

PHYSICAL SURFACE FEATURES AND CHEMICAL DENSITY OF DRY BACTERIAL SPORES

E Berlin 1, H R Curran 1, M J Pallansch 1
PMCID: PMC278562  PMID: 14080768

Abstract

Berlin, E. (U.S. Department of Agriculture, Washington, D.C.), H. R. Curran, and M. J. Pallansch. Physical surface features and chemical density of dry bacterial spores. J. Bacteriol. 86:1030–1036. 1963.—Gas-displacement and gas-adsorption techniques were used to determine the chemical density and physical surface properties of the spores of Bacillus subtilis 15U, B. cereus 720, and B. stearothermophilus 1518 held in the dry state. Neither the observed densities nor the specific surface areas measured could be correlated with the heat resistance of the spores studied. Analysis of data obtained from a study of the adsorption of nitrogen by spores held at −195 C led to the postulate that the surface of the dry spore is relatively smooth and impervious, and is characterized by the presence of a few pores having radii of approximately 300 A. The presence of such orifices could account for the permeability of the spore form reported by other workers.

Full text

PDF
1030

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. ANAND J. C. Density of bacterial spores & their destruction rate by heat. J Sci Ind Res (C) 1961 Dec;20C:353–355. [PubMed] [Google Scholar]
  2. BLACK S. H., GERHARDT P. Permeability of bacterial spores. I. Characterization of glucose uptake. J Bacteriol. 1961 Nov;82:743–749. doi: 10.1128/jb.82.5.743-749.1961. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. BLACK S. H., GERHARDT P. Permeability of bacterial spores. III. Permeation relative to germination. J Bacteriol. 1962 Feb;83:301–308. doi: 10.1128/jb.83.2.301-308.1962. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. BLACK S. H., GERHARDT P. Permeability of bacterial spores. IV. Water content, uptake, and distribution. J Bacteriol. 1962 May;83:960–967. doi: 10.1128/jb.83.5.960-967.1962. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. GERHARDT P., BLACK S. H. Permeability of bacterial spores. II. Molecular variables affecting solute permeation. J Bacteriol. 1961 Nov;82:750–760. doi: 10.1128/jb.82.5.750-760.1961. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Knaysi G. Process of Sporulation in Strain of Bacillus cereus. J Bacteriol. 1946 Feb;51(2):187–197. [PMC free article] [PubMed] [Google Scholar]
  7. SUGIYAMA H. Studies on factors affecting the heat resistance of spores of Clostridium botulinum. J Bacteriol. 1951 Jul;62(1):81–96. doi: 10.1128/jb.62.1.81-96.1951. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from Journal of Bacteriology are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES