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. Author manuscript; available in PMC: 2010 Jul 21.
Published in final edited form as: Biochemistry. 2009 Jul 21;48(28):6598–6609. doi: 10.1021/bi802221h

Figure 4.

Figure 4

NMR-derived hydrogen–deuterium exchange data of Fis1ΔTM at 14.2 T, 25 °C, and pH 7.4 and 5.0 as a function of residue number. The rate constants for exchange of the amide backbone with solvent deuterons, log10 kex, are derived from a series of HSQC experiments conducted with a 15N-labeled sample after dissolution in D2O. Note the N-terminal region and helix 6 are not protected from solvent exchange at pH 7.4 but are at pH 5.0. Residues marked with an asterisk (38, 40, 42, 46, 49, 50, 53, 57, 59–61, 64, 67, 83–85,89,90,92,100,101,109, and 112) showed “super protection”.