Skip to main content
Journal of Bacteriology logoLink to Journal of Bacteriology
. 1963 Dec;86(6):1145–1151. doi: 10.1128/jb.86.6.1145-1151.1963

STARCH GEL ELECTROPHORESIS OF A FRACTION OF CERTAIN OF THE PLEUROPNEUMONIA-LIKE GROUP OF MICROORGANISMS

Richard C Fowler 1, Don W Coble 1, Norman C Kramer 1, Thomas McP Brown 1
PMCID: PMC278598  PMID: 14086081

Abstract

Fowler, Richard C. (George Washington University School of Medicine, Washington, D.C.), Don W. Coble, Norman C. Kramer, and Thomas McP. Brown. Starch gel electrophoresis of a fraction of certain of the pleuropneumonia-like group of microorganisms. J. Bacteriol. 86:1145–1151. 1963.—Starch gel electrophoresis is suggested as a means of further characterizing strains of the pleuropneumonia-like group of microorganisms (PPLO), defined herein as including both the various “L” forms of bacteria and the Mycoplasmataceae. The techniques used may be varied to “select” other groups of proteins or classes of compounds (e.g., glycoproteins, lipoproteins, and enzymes). Only the results by starch gel electrophoresis on the soluble portion of sonically treated, washed cultures, grown in a pancreatic digest of beef heart enriched with bovine serum, are reported. As yet, differences appear more significant than similarities among the electrophoretically separable proteins. The demonstration of characteristic protein patterns for each strain studied suggests possibilities of further dividing serological groups into sero-logically distinct subtypes. Tentative migration values (Ēf) have been assigned to the protein bands for convenience in designation. Numerous interrelationships are thereby suggested.

Full text

PDF
1145

Images in this article

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. BAILEY J. S., CLARK H. W., FELTS W. R., FOWLER R. C., BROWN T. M. Antigenic properties of pleuropneumonia-like organisms from tissue cell cultures and the human genital area. J Bacteriol. 1961 Oct;82:542–547. doi: 10.1002/path.1700820236. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Brown J. H. Media Prepared by the Pancreatic Digestion of Meat. J Bacteriol. 1948 Jun;55(6):871–872. [PMC free article] [PubMed] [Google Scholar]
  3. CARD D. H. PPLO of human genital origin; serological classification of strains and antibody distribution in man. Br J Vener Dis. 1959 Mar;35(1):27–34. doi: 10.1136/sti.35.1.27. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. CHANOCK R. M., HAYFLICK L., BARILE M. F. Growth on artificial medium of an agent associated with atypical pneumonia and its identification as a PPLO. Proc Natl Acad Sci U S A. 1962 Jan 15;48:41–49. doi: 10.1073/pnas.48.1.41. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. CORIELL L. L., FABRIZIO D. P., WILSON S. R. Comparison of PPLO strains from tissue culture by complement fixation. Ann N Y Acad Sci. 1960 Jan 15;79:574–580. doi: 10.1111/j.1749-6632.1960.tb42726.x. [DOI] [PubMed] [Google Scholar]
  6. Clark H. W., Bailey J. S., Fowler R. C., Brown T. M. IDENTIFICATION OF MYCOPLASMATACEAE BY THE FLUORESCENT ANTIBODY METHOD. J Bacteriol. 1963 Jan;85(1):111–118. doi: 10.1128/jb.85.1.111-118.1963. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. MALIZIA W. F., BARILE M. F., RIGGS D. B. Immunofluorescence of pleuropneumonia-like organisms isolated from tissue cell cultures. Nature. 1961 Jul 8;191:190–191. doi: 10.1038/191190a0. [DOI] [PubMed] [Google Scholar]
  8. MOROWITZ H. J., TOURTELLOTTE M. E. The smallest living cells. Sci Am. 1962 Mar;206:117–126. doi: 10.1038/scientificamerican0362-117. [DOI] [PubMed] [Google Scholar]
  9. PERT J. H., ENGLE R. E., Jr, WOODS K. R., SLEISENGER M. H. Preliminary studies on quantitative zone electrophoresis in starch gel. J Lab Clin Med. 1959 Oct;54:572–584. [PubMed] [Google Scholar]
  10. POULIK M. D. Starch gel electrophoresis in a discontinous system of buffers. Nature. 1957 Dec 28;180(4600):1477–1479. doi: 10.1038/1801477a0. [DOI] [PubMed] [Google Scholar]
  11. SMITHIES O. An improved procedure for starch-gel electrophoresis: further variations in the serum proteins of normal individuals. Biochem J. 1959 Mar;71(3):585–587. doi: 10.1042/bj0710585. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from Journal of Bacteriology are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES