Abstract
Fiock, Mary A. (Fort Detrick, Frederick, Md.), Allen Yarinsky, and James T. Duff. Studies on immunity to toxins of Clostridium botulinum. VII. Purification and detoxification of trypsin-activated type E toxin. J. Bacteriol. 82:66–71. 1961.—A procedure was described for production, purification, and conversion to toxoid of the Clostridium botulinum type E trypsin-treated toxin. A medium containing 1.5% trypticase, 0.5% yeast extract, 0.075% cysteine hydrochloride, and 1.0% glucose consistently yielded activated culture toxicities of 300,000 mouse intraperitoneal ld50/ml. Purification of the toxin was accomplished by precipitation with ammonium sulfate, extraction with calcium chloride, and reprecipitation with ethanol in the cold. The resulting partially purified toxin had a specific activity of 45,000,000 ld50/mg nitrogen. Purified toxins were converted to toxoid by incubation with formalin and adsorbed on aluminum phosphate. Good immune responses were obtained to the toxoids in mice, guinea pigs, and rabbits.
Full text
PDF
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- CARDELLA M. A., DUFF J. T., WINGFIELD B. H., GOTTFRIED C. Studies on immunity to toxins of Clostridium botulinum. VI. Purification and detoxification of type D toxin and the immunological response to toxoid. J Bacteriol. 1960 Mar;79:372–378. doi: 10.1128/jb.79.3.372-378.1960. [DOI] [PMC free article] [PubMed] [Google Scholar]
- DOLMAN C. E., CHANG H., KERR D. E., SHEARER A. R. Fish-borne and type E botulism: two cases due to home-pickled herring. Can J Public Health. 1950 Jun;41(6):215–229. [PubMed] [Google Scholar]
- DUFF J. T., KLERER J., BIBLER R. H., MOORE D. E., GOTTFRIED C., WRIGHT G. G. Studies on immunity to toxins of Clostridium botulinum. II. Production and purification of type B toxin for toxoid. J Bacteriol. 1957 May;73(5):597–601. doi: 10.1128/jb.73.5.597-601.1957. [DOI] [PMC free article] [PubMed] [Google Scholar]
- DUFF J. T., WRIGHT G. G., KLERER J., MOORE D. E., BIBLER R. H. Studies on immunity to toxins of Clostridium botulinum. I. A simplified procedure for isolation of type A toxin. J Bacteriol. 1957 Jan;73(1):42–47. doi: 10.1128/jb.73.1.42-47.1957. [DOI] [PMC free article] [PubMed] [Google Scholar]
- DUFF J. T., WRIGHT G. G., YARINSKY A. Activation of Clostridium botulinum type E toxin by trypsin. J Bacteriol. 1956 Oct;72(4):455–460. doi: 10.1128/jb.72.4.455-460.1956. [DOI] [PMC free article] [PubMed] [Google Scholar]
- GORDON M., FIOCK M. A., YARINSKY A., DUFF J. T. Studies on immunity to toxins of Clostridium botulinum. III. Preparation, purification, and detoxification of type E toxin. J Bacteriol. 1957 Oct;74(4):533–538. doi: 10.1128/jb.74.4.533-538.1957. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Lamanna C., Glassman H. N. The Isolation of Type B Botulinum Toxin. J Bacteriol. 1947 Nov;54(5):575–584. doi: 10.1128/jb.54.5.575-584.1947. [DOI] [PMC free article] [PubMed] [Google Scholar]
- WAGMAN J., BATEMAN J. B. The behavior of the bolulinus toxins in the ultracentrifuge. Arch Biochem Biophys. 1951 May;31(3):424–430. doi: 10.1016/0003-9861(51)90158-0. [DOI] [PubMed] [Google Scholar]
- WAGMAN J. Isolation and sedimentation study of low molecular weight forms of type A Botulins toxin. Arch Biochem Biophys. 1954 May;50(1):104–112. doi: 10.1016/0003-9861(54)90013-2. [DOI] [PubMed] [Google Scholar]
- WRIGHT G. G., DUFF J. T., FIOCK M. A., DEVLIN H. B., SODERSTROM R. L. Studies on immunity to toxins of Clostridium botulinum. V. Detoxification of purified type A and type B toxins, and the antigenicity of univalent and bivalent aluminium phosphate adsorbed toxoids. J Immunol. 1960 Apr;84:384–389. [PubMed] [Google Scholar]