Abstract
Sagers, Richard D. (University of Illinois, Urbana), Moshe Benziman, and I. C. Gunsalus. Acetate formation in Clostridium acidiurici: Acetokinase. J. Bacteriol. 82:233–238. 1961.—To define the energy-yielding reaction(s) during fermentation of purines by Clostridium acidi-urici, an eightfold purified acetokinase was obtained by protamine and ammonium sulfate fractionation of crude extracts. Enzyme activity was determined by measuring the disappearance of acetyl phosphate using adenosine diphosphate (ADP) as phosphate acceptor or by following the generation of acetyl phosphate from adenosine triphosphate (ATP) + acetate. The optimal pH for the reaction was observed to be 7.4. Km values for acetyl phosphate, ADP, and magnesium ions were shown to be 2.1 × 10−3m, 3.2 × 10−3m, and 2.4 × 10−3m, respectively. Acetyl phosphate disappearance is dependent upon ADP and is stoichiometric with addition of the latter. The acetokinase reaction probably represents the major energy-yielding reaction during purine fermentation by this organism. A pathway for acetate generation from formiminoglycine is proposed, and evidence for a number of enzyme activities intermediate between formiminoglycine degradation and acetate formation is given. The enzyme activities demonstrated are compatible with the over-all purine fermentation rate by whole cells.
Full text
PDF
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- BECK J. V., SAGERS R. D. Studies on the formation of formate, glycine, serine, pyruvate and acetate from purines by Clostridium acidi-urici. J Bacteriol. 1956 Aug;72(2):199–208. doi: 10.1128/jb.72.2.199-208.1956. [DOI] [PMC free article] [PubMed] [Google Scholar]
- BENZIMAN M., SAGERS R. D., GUNSALUS I. C. L-serine specific dehydrase from Clostridium acidi-urici. J Bacteriol. 1960 Apr;79:474–479. doi: 10.1128/jb.79.4.474-479.1960. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Barker H. A., Beck J. V. Clostridium acidi-uridi and Clostridium cylindrosporum, Organisms Fermenting Uric Acid and Some Other Purines. J Bacteriol. 1942 Mar;43(3):291–304. doi: 10.1128/jb.43.3.291-304.1942. [DOI] [PMC free article] [PubMed] [Google Scholar]
- RABINOWITZ J. C., PRICER W. E., Jr An enzymatic method for the determination of formic acid. J Biol Chem. 1957 Nov;229(1):321–328. [PubMed] [Google Scholar]
- ROSE I. A., GRUNBERG-MANAGO M., KOREY S. R., OCHOA S. Enzymatic phosphorylation of acetate. J Biol Chem. 1954 Dec;211(2):737–756. [PubMed] [Google Scholar]
- STADTMAN E. R., LIPMANN F. Acetyl phosphate synthesis by reaction of isopropenyl acetate and phosphoric acid. J Biol Chem. 1950 Aug;185(2):549–551. [PubMed] [Google Scholar]
- STADTMAN E. R., NOVELLI G. D., LIPMANN F. Coenzyme A function in and acetyl transfer by the phosphotransacetylase system. J Biol Chem. 1951 Jul;191(1):365–376. [PubMed] [Google Scholar]