Abstract
Blobel, Hans (University of Wisconsin, Madison). Isolation and characterization of deoxyribonucleic acid from a strain of Staphylococcus aureus. J. Bacteriol. 82:425–429. 1961.—Highly polymerized deoxyribonucleic acid was extracted from washed cells of Staphylococcus aureus with a mixture of equal parts of phenol and 2 m NaCl at pH 7.4. The aqueous phase was treated twice with phenol and the deoxyribonucleic acid precipitated with an equal volume of 2-ethoxyethanol. Residual ribonucleic acid was removed by treatment with ribonuclease and subsequent dialysis. Deoxyribonucleic acid was reprecipitated with 2-ethoxyethanol. The final product contained less than 1% protein. The deoxyribonucleic acid obtained from S. aureus strain S44 had a (adenosine + thymine)/(guanine + cytosine) base ratio of 1.98. Intrinsic viscosity in 0.15 m NaCl + 0.015 m sodium citrate was approximately 76 dl/g. The sedimentation coefficient, S20w, was close to 25 S.
Full text
PDF
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- BLANK H., McCARTHY P. L., DeLAMATER E. D. A non-vacuum freezing-dehydrating technic for histology, autoradiography and microbial cytology. Stain Technol. 1951 Jul;26(3):193–197. doi: 10.3109/10520295109113207. [DOI] [PubMed] [Google Scholar]
- CATLIN B. W., CUNNINGHAM L. S. Studies of extracellular and intracellular bacterial deoxyribonucleic acids. J Gen Microbiol. 1958 Dec;19(3):522–539. doi: 10.1099/00221287-19-3-522. [DOI] [PubMed] [Google Scholar]
- DJORDJEVIC B., SZYBALSKI W. Genetics of human cell lines. III. Incorporation of 5-bromo- and 5-iododeoxyuridine into the deoxyribonucleic acid of human cells and its effect on radiation sensitivity. J Exp Med. 1960 Sep 1;112:509–531. doi: 10.1084/jem.112.3.509. [DOI] [PMC free article] [PubMed] [Google Scholar]
- KIRBY K. S. A new method for the isolation of deoxyribonucleic acids; evidence on the nature of bonds between deoxyribonucleic acid and protein. Biochem J. 1957 Jul;66(3):495–504. doi: 10.1042/bj0660495. [DOI] [PMC free article] [PubMed] [Google Scholar]
- McINTOSH D. L. A Feulgen-carmine technic for staining fungus chromosomes. Stain Technol. 1954 Jan;29(1):29–31. doi: 10.3109/10520295409115434. [DOI] [PubMed] [Google Scholar]
- ROBINOW C. F. The chromatin bodies of bacteria. Bacteriol Rev. 1956 Dec;20(4):207–242. doi: 10.1128/br.20.4.207-242.1956. [DOI] [PMC free article] [PubMed] [Google Scholar]