Abstract
Soffer, Richard L. (Stanford University, Palo Alto, Calif.). Enzymatic expression of genetic units of function concerned with galactose metabolism in Escherichia coli. J. Bacteriol. 82:471–478. 1961.—A series of genetically characterized galactose-negative mutants of Escherichia coli strain K12 were studied with respect to the enzymes of the Leloir pathway for utilization of this sugar. The functions pertaining to the three genetically defined cistrons were found to relate, respectively, to the expression of galactokinase, galactose 1-phosphate uridyl transferase, and uridine diphosphate galactose 4-epimerase. Mutants defective in the latter enzyme represent a new biochemical class in this strain of E. coli. Most of the mutants defective in galactokinase were found to exhibit significantly elevated levels of galactose 1-phosphate uridyl transferase as compared with the wild type when grown under conditions of induction. A suppressed transferase-deficient mutant was found to have regained this enzymatic activity.
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Selected References
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- AXELROD J., KALCKAR H. M., MAXWELL E. S., STROMINGER J. L. Enzymatic formation of uridine diphosphoglucuronic acid. J Biol Chem. 1957 Jan;224(1):79–90. [PubMed] [Google Scholar]
- BUTTIN G., JACOB F., MONOD J. [Constituent synthesis of galactokinase following the development of lambda bacteriophages in Escherichia coli K 12]. C R Hebd Seances Acad Sci. 1960 Mar 28;250:2471–2473. [PubMed] [Google Scholar]
- FUKASAWA T., NIKAIDO H. Galactose-sensitive mutants of Salmonella. Nature. 1959 Oct 10;184(Suppl 15):1168–1169. doi: 10.1038/1841168a0. [DOI] [PubMed] [Google Scholar]
- HORECKER B. L., THOMAS J., MONOD J. Galactose transport in Escherichia coli. I. General properties as studied in a galactokinaseless mutant. J Biol Chem. 1960 Jun;235:1580–1585. [PubMed] [Google Scholar]
- KALCKAR H. M. Uridinediphospho galactose; metabolism, enzymology, and biology. Adv Enzymol Relat Subj Biochem. 1958;20:111–134. doi: 10.1002/9780470122655.ch4. [DOI] [PubMed] [Google Scholar]
- KURAHASHI K. Enzyme formation in galactose-negative mutants of Escherichia coli. Science. 1957 Jan 18;125(3238):114–116. doi: 10.1126/science.125.3238.114. [DOI] [PubMed] [Google Scholar]
- KURAHASHI K., SUGIMURA A. Purification and properties of galactose 1-phosphate uridyl transferase from Escherichia coli. J Biol Chem. 1960 Apr;235:940–946. [PubMed] [Google Scholar]
- KURAHASHI K., WAHBA A. J. Interference with growth of certain Escherichia coli mutants by galactose. Biochim Biophys Acta. 1958 Nov;30(2):298–302. doi: 10.1016/0006-3002(58)90054-4. [DOI] [PubMed] [Google Scholar]
- Kalckar H. M., Kurahashi K., Jordan E. HEREDITARY DEFECTS IN GALACTOSE METABOLISM IN ESCHERICHIA COLI MUTANTS, I. DETERMINATION OF ENZYME ACTIVITIES. Proc Natl Acad Sci U S A. 1959 Dec;45(12):1776–1786. doi: 10.1073/pnas.45.12.1776. [DOI] [PMC free article] [PubMed] [Google Scholar]
- LELOIR L. F., TRUCCO R. E. The formation of glucose diphosphate by Escherichia coli. Arch Biochem. 1949 Nov;24(1):65–74. [PubMed] [Google Scholar]
- LOWRY O. H., ROSEBROUGH N. J., FARR A. L., RANDALL R. J. Protein measurement with the Folin phenol reagent. J Biol Chem. 1951 Nov;193(1):265–275. [PubMed] [Google Scholar]
- MONOD J., COHEN-BAZIRE G., COHN M. Sur la biosynthèse de la beta-galactosidase (lactase) chez Escherichia coli; la spécificité de l'induction. Biochim Biophys Acta. 1951 Nov;7(4):585–599. doi: 10.1016/0006-3002(51)90072-8. [DOI] [PubMed] [Google Scholar]
- MUNCH-PETERSEN A., KALCKAR H. M., CUTOLO E., SMITH E. E. Uridyl transferases and the formation of uridine triphosphate; enzymic production of uridine triphosphate: uridine diphosphoglucose pyrophosphorolysis. Nature. 1953 Dec 5;172(4388):1036–1037. doi: 10.1038/1721036a0. [DOI] [PubMed] [Google Scholar]
- Morse M L, Lederberg E M, Lederberg J. Transduction in Escherichia Coli K-12. Genetics. 1956 Jan;41(1):142–156. doi: 10.1093/genetics/41.1.142. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Morse M L, Lederberg E M, Lederberg J. Transductional Heterogenotes in Escherichia Coli. Genetics. 1956 Sep;41(5):758–779. doi: 10.1093/genetics/41.5.758. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Yarmolinsky M. B., Wiesmeyer H., Kalckar H. M., Jordan E. HEREDITARY DEFECTS IN GALACTOSE METABOLISM IN ESCHERICHIA COLI MUTANTS, II. GALACTOSE-INDUCED SENSITIVITY. Proc Natl Acad Sci U S A. 1959 Dec;45(12):1786–1791. doi: 10.1073/pnas.45.12.1786. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Yarmolinsky M. B., Wiesmeyer H. REGULATION BY COLIPHAGE LAMBDA OF THE EXPRESSION OF THE CAPACITY TO SYNTHESIZE A SEQUENCE OF HOST ENZYMES. Proc Natl Acad Sci U S A. 1960 Dec;46(12):1626–1645. doi: 10.1073/pnas.46.12.1626. [DOI] [PMC free article] [PubMed] [Google Scholar]