Abstract
Hinze, Harry C. (University of Wisconsin, Madison), and Duard L. Walker. Variation of herpes simplex virus in persistently infected tissue cultures. J. Bacteriol. 82:498–504. 1961.—Cultures of human conjunctiva, HeLa, and KB cells infected with herpes simplex virus (HF strain) were cultured for prolonged periods in medium containing low levels of antibody. After continuous culture of the infected cell lines for 6 months, two major changes were noted in the character of the virus present in the cultures. These changes consisted of an alteration in the type of cytopathic effect produced by the virus and marked loss of virulence for mice. Further study of the virus from the cultures revealed that it was still antigenically similar to the original strain of herpes virus. It was found that the variant produced an altered, proliferative type of cytopathic effect only in the presence of herpes antibody. The variant multiplied more slowly in human conjunctiva cells in culture than did the parent virus, and the variant had lost practically all capacity for multiplication in the brain of mice.
Full text
PDF
Images in this article
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- CHANG R. S. M. Continuous subcultivation of epithelial-like cells from normal human tissues. Proc Soc Exp Biol Med. 1954 Nov;87(2):440–443. doi: 10.3181/00379727-87-21406. [DOI] [PubMed] [Google Scholar]
- EAGLE H. Nutrition needs of mammalian cells in tissue culture. Science. 1955 Sep 16;122(3168):501–514. doi: 10.1126/science.122.3168.501. [DOI] [PubMed] [Google Scholar]
- EAGLE H. Propagation in a fluid medium of a human epidermoid carcinoma, strain KB. Proc Soc Exp Biol Med. 1955 Jul;89(3):362–364. doi: 10.3181/00379727-89-21811. [DOI] [PubMed] [Google Scholar]
- FINKELSTEIN R. A., ALLEN R., SULKIN S. E. Inhibition of herpes simplex virus by normal serum: its relationship to the properdin system. J Infect Dis. 1959 Mar-Apr;104(2):184–192. doi: 10.1093/infdis/104.2.184. [DOI] [PubMed] [Google Scholar]
- GINSBERG H. S. The significance of the viral carrier state in tissue culture systems. Prog Med Virol. 1958;1:36–58. [PubMed] [Google Scholar]
- GRAY A., TOKUMARU T., SCOTT T. F. M. Different cytopathogenic effects observed in HeLa cells infected with herpes simplex virus. Arch Gesamte Virusforsch. 1958;8(1):59–76. doi: 10.1007/BF01242313. [DOI] [PubMed] [Google Scholar]
- HOGGAN M. D., ROIZMAN B., ROANE P. R., Jr Further studies of variants of herpes simplex virus that produce syncytia or pocklike lesions in cell cultures. Am J Hyg. 1961 Jan;73:114–122. doi: 10.1093/oxfordjournals.aje.a120162. [DOI] [PubMed] [Google Scholar]
- HOGGAN M. D., ROIZMAN B. The isolation and properties of a variant of Herpes simplex producing multinucleated giant cells in monolayer cultures in the presence of antibody. Am J Hyg. 1959 Sep;70:208–219. doi: 10.1093/oxfordjournals.aje.a120071. [DOI] [PubMed] [Google Scholar]
- MORGAN J. F., MORTON H. J., PARKER R. C. Nutrition of animal cells in tissue culture; initial studies on a synthetic medium. Proc Soc Exp Biol Med. 1950 Jan;73(1):1–8. doi: 10.3181/00379727-73-17557. [DOI] [PubMed] [Google Scholar]
- SCHERER W. F., SYVERTON J. T., GEY G. O. Studies on the propagation in vitro of poliomyelitis viruses. IV. Viral multiplication in a stable strain of human malignant epithelial cells (strain HeLa) derived from an epidermoid carcinoma of the cervix. J Exp Med. 1953 May;97(5):695–710. doi: 10.1084/jem.97.5.695. [DOI] [PMC free article] [PubMed] [Google Scholar]
- SCOTT T. F., McLEOD D. L. Cellular responses to infection with strains of herpes simplex virus. Ann N Y Acad Sci. 1959 Jul 21;81:118–128. doi: 10.1111/j.1749-6632.1959.tb49300.x. [DOI] [PubMed] [Google Scholar]
- SCOTT T. F., McLEOD D. L., TOKUMARU T. A biologic comparison of two strains of Herpesvirus hominis. J Immunol. 1961 Jan;86:1–12. [PubMed] [Google Scholar]
- WHEELER C. E., CANBY C. M. Influence of specific antibody on herpes simplex infections in tissue culture. AMA Arch Derm. 1959 Jan;79(1):86–95. doi: 10.1001/archderm.1959.01560130088010. [DOI] [PubMed] [Google Scholar]