Abstract
Griffin, Charles W. (Baltimore Biological Laboratory, Baltimore, Md.), Theodore R. Carski, and George S. Warner. Labeling procedures employing crystalline fluorescein isothiocyanate. J. Bacteriol. 82:534–537. 1961.—The effect of altering the ratio of dye to protein in the labeling of three immune globulins with crystalline fluorescein isothiocyanate was the purpose of the study. Direct staining of group A streptococci and rabies-infected mouse brain, and indirect staining of Treponema pallidum (Nichols strain) comprised the three immunological systems selected for investigation. Maximal specific staining and minimal nonspecific background staining were demonstrated by the use of low dye-to-protein labeling ratios.
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Selected References
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- Beutner E. H. IMMUNOFLUORESCENT STAINING: THE FLUORESCENT ANTIBODY METHOD. Bacteriol Rev. 1961 Mar;25(1):49–76. doi: 10.1128/br.25.1.49-76.1961. [DOI] [PMC free article] [PubMed] [Google Scholar]
- CHADWICK C. S., NAIRN R. C. Fluorescent protein tracers: the unreacted fluorescent material in fluorescein conjugates and studies of conjugates with other green fluorochromes. Immunology. 1960 Oct;3:363–370. [PMC free article] [PubMed] [Google Scholar]
- COONS A. H., KAPLAN M. H. Localization of antigen in tissue cells; improvements in a method for the detection of antigen by means of fluorescent antibody. J Exp Med. 1950 Jan 1;91(1):1–13. doi: 10.1084/jem.91.1.1. [DOI] [PMC free article] [PubMed] [Google Scholar]
- CURTAIN C. C. Electrophoresis of fluorescent antibody. Nature. 1958 Nov 8;182(4645):1305–1306. doi: 10.1038/1821305a0. [DOI] [PubMed] [Google Scholar]
- DEACON W. E., FALCONE V. H., HARRIS A. A fluorescent test for treponemal antibodies. Proc Soc Exp Biol Med. 1957 Nov;96(2):477–480. doi: 10.3181/00379727-96-23512. [DOI] [PubMed] [Google Scholar]
- DEACON W. E., FREEMAN E. M., HARRIS A. Fluorescent treponemal antibody test. Modification based on quantitation (FTA-200). Proc Soc Exp Biol Med. 1960 Apr;103:827–829. doi: 10.3181/00379727-103-25685. [DOI] [PubMed] [Google Scholar]
- FELTON L. C., McMILLION C. R. Chromatographically pure fluorescein and tetramethylrhodamine isothiocyanates. Anal Biochem. 1961 Apr;2:178–180. doi: 10.1016/0003-2697(61)90068-9. [DOI] [PubMed] [Google Scholar]
- GOLDSTEIN G., SLIZYS I. S., CHASE M. W. Studies on fluorescent antibody staining. I. Non-specific fluorescence with fluorescein-coupled sheep anti-rabbit globulins. J Exp Med. 1961 Jul 1;114:89–110. doi: 10.1084/jem.114.1.89. [DOI] [PMC free article] [PubMed] [Google Scholar]
- GOLDWASSER R. A., KISSLING R. E., CARSKI T. R., HOSTY T. S. Fluorescent antibody staining of rabies virus antigens in the salivary glands of rabid animals. Bull World Health Organ. 1959;20:579–588. [PMC free article] [PubMed] [Google Scholar]
- GOLDWASSER R. A., SHEPARD C. C. Staining of complement and modification of fluorescent antibody procedures. J Immunol. 1958 Feb;80(2):122–131. [PubMed] [Google Scholar]
- MARSHALL J. D., EVELAND W. C., SMITH C. W. Superiority of fluorescein isothiocyanate (Riggs) for fluorescent-antibody technic with a modification of its application. Proc Soc Exp Biol Med. 1958 Aug-Sep;98(4):898–900. doi: 10.3181/00379727-98-24222. [DOI] [PubMed] [Google Scholar]
- MOODY M. D., ELLIS E. C., UPDYKE E. L. Staining bacterial smears with fluorescent antibody. IV. Grouping streptococci with fluorescent antibody. J Bacteriol. 1958 May;75(5):553–560. doi: 10.1128/jb.75.5.553-560.1958. [DOI] [PMC free article] [PubMed] [Google Scholar]
- RIGGS J. L., SEIWALD R. J., BURCKHALTER J. H., DOWNS C. M., METCALF T. G. Isothiocyanate compounds as fluorescent labeling agents for immune serum. Am J Pathol. 1958 Nov-Dec;34(6):1081–1097. [PMC free article] [PubMed] [Google Scholar]