Abstract
Campbell, J. N. (American Meat Institute Foundation, Chicago, Ill.), James B. Evans, Jerome J. Perry, and C. F. Niven, Jr. Effect of ammonium ion on growth and metabolism of Micrococcus sodonensis. J. Bacteriol. 82:823–827. 1961.—When Micrococcus sodonensis was grown in a synthetic medium deficient in ammonia, large quantities of α-keto acids, chiefly α-ketoglutaric, accumulated in the medium. The addition of ammonium chloride to this medium prevented such accumulation and also supported increased growth, even in the presence of excess glutamate. Neither potassium nor sodium would substitute for ammonia in producing this effect.
The results indicate that this microorganism has a specific requirement for the ammonium ion in its growth and metabolism. If not supplied exogenously, ammonia is made available by deamination of glutamic acid. The exact route of incorporation of ammonia is unknown.
Full text
PDF
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- AARONSON S. Biotin assay with a coccus, Micrococcus sodonensis, nov. sp. J Bacteriol. 1955 Jan;69(1):67–69. doi: 10.1128/jb.69.1.67-69.1955. [DOI] [PMC free article] [PubMed] [Google Scholar]
- CAMPBELL J. N., EVANS J. B., PERRY J. J., NIVEN C. F., Jr An extracellular material elaborated by Micrococcus sodonensis. J Bacteriol. 1961 Dec;82:828–837. doi: 10.1128/jb.82.6.828-837.1961. [DOI] [PMC free article] [PubMed] [Google Scholar]
- HALPERN Y. S., UMBARGER H. E. Conversion of ammonia to amino groups in Escherichia coli. J Bacteriol. 1960 Sep;80:285–288. doi: 10.1128/jb.80.3.285-288.1960. [DOI] [PMC free article] [PubMed] [Google Scholar]
- KUN E., GARCIA-HERNANDEZ M. Identification and quantitative determination of keto acids by paper chromatography. Biochim Biophys Acta. 1957 Jan;23(1):181–186. doi: 10.1016/0006-3002(57)90301-3. [DOI] [PubMed] [Google Scholar]
- PERRY J. J., EVANS J. B. Oxidative metabolism of lactate and acetate by Micrococcus sodonensis. J Bacteriol. 1960 Jan;79:113–118. doi: 10.1128/jb.79.1.113-118.1960. [DOI] [PMC free article] [PubMed] [Google Scholar]
- STRAUSS B. S. The nature of the lesion in the succinate-requiring mutants of Neurospora crassa: interaction between carbohydrate and nitrogen metabolism. J Gen Microbiol. 1956 Jul;14(3):494–511. doi: 10.1099/00221287-14-3-494. [DOI] [PubMed] [Google Scholar]