Skip to main content
NCBI home page
Journal of Bacteriology logoLink to Journal of Bacteriology
. 1961 Dec;82(6):927–932. doi: 10.1128/jb.82.6.927-932.1961

STUDIES ON THERMOPHILIC SULFATE-REDUCING BACTERIA II.

Hydrogenase Activity of Clostridium nigrificans1

J M Akagi a,2, L Leon Campbell a,3
PMCID: PMC279278  PMID: 13859876

Abstract

Akagi, J. M. (Western Reserve University, Cleveland, Ohio) and L. Leon Campbell. Studies on thermophilic sulfate-reducing bacteria. II. Hydrogenase activity of Clostridium nigrificans. J. Bacteriol. 82:927–932. 1961.—The hydrogenase of Clostridium nigrificans has been found to be associated with the cell-free particulate fraction which can be sedimented at 105,000 × g in 1 hr. The specific activity of this fraction was increased 2 to 3 fold over that of the crude extract. It was not found possible to liberate the enzyme from the particulate fraction by methods of enzymatic digestion, chemical extraction, or physical disruption. The optimum temperature for H2 utilization using benzyl viologen as an electron acceptor was found to be 55 C, and the optimum pH range was 7 to 8. Employing metal complexing agents it was found that the enzyme required Fe++ ions for H2 utilization. In contrast, Fe++ ions were not required to catalyze the evolution of H2 from reduced methyl viologen. The role of Fe++ ions in the hydrogenase activity of this organism is discussed.

Full text

PDF
927

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. CAMPBELL L. L., Jr, FRANK H. A., HALL E. R. Studies on thermophilic sulfate reducing bacteria. I. Identification of Sporovibrio desulfuricans as Clostridium nigrificans. J Bacteriol. 1957 Apr;73(4):516–521. doi: 10.1128/jb.73.4.516-521.1957. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. GEST H. Oxidation and evolution of molecular hydrogen by microorganisms. Bacteriol Rev. 1954 Mar;18(1):43–73. doi: 10.1128/br.18.1.43-73.1954. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. KRASNA A. I., RIKLIS E., RITTENBERG D. The purification and properties of the hydrogenase of Desulfovibrio desulfuricans. J Biol Chem. 1960 Sep;235:2717–2720. [PubMed] [Google Scholar]
  4. LOWRY O. H., ROSEBROUGH N. J., FARR A. L., RANDALL R. J. Protein measurement with the Folin phenol reagent. J Biol Chem. 1951 Nov;193(1):265–275. [PubMed] [Google Scholar]
  5. MORTON R. K. Separation and purification of enzymes associated with insoluble particles. Nature. 1950 Dec 30;166(4235):1092–1095. doi: 10.1038/1661092a0. [DOI] [PubMed] [Google Scholar]
  6. PECK H. D., Jr, GEST H. A new procedure for assay of bacterial hydrogenases. J Bacteriol. 1956 Jan;71(1):70–80. doi: 10.1128/jb.71.1.70-80.1956. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. PECK H. D., Jr, GEST H. Hydrogenase of Clostridium butylicum. J Bacteriol. 1957 Apr;73(4):569–580. doi: 10.1128/jb.73.4.569-580.1957. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. POSTGATE J. R. Cytochrome c3 and desulphoviridin; pigments of the anaerobe Desulphovibrio desulphuricans. J Gen Microbiol. 1956 Jul;14(3):545–572. doi: 10.1099/00221287-14-3-545. [DOI] [PubMed] [Google Scholar]
  9. POSTGATE J. R. Iron and the synthesis of cytochrome c3. J Gen Microbiol. 1956 Aug;15(1):186–193. doi: 10.1099/00221287-15-1-186. [DOI] [PubMed] [Google Scholar]
  10. POSTGATE J. R. On the nutrition of Desulphovibrio desulphuricans. J Gen Microbiol. 1951 Oct;5(4):714–724. doi: 10.1099/00221287-5-4-714. [DOI] [PubMed] [Google Scholar]
  11. Peck H. D., Pietro A. S., Gest H. ON THE MECHANISM OF HYDROGENASE ACTION. Proc Natl Acad Sci U S A. 1956 Jan;42(1):13–19. doi: 10.1073/pnas.42.1.13. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Proceedings of the Biochemical Society. Biochem J. 1954 Feb;56(2):xi–xviii. [PMC free article] [PubMed] [Google Scholar]
  13. SADANA J. C., JAGANNATHAN V. Purification and properties of the hydrogenase of Desulfovibrio desulfuricans. Biochim Biophys Acta. 1956 Mar;19(3):440–452. doi: 10.1016/0006-3002(56)90467-x. [DOI] [PubMed] [Google Scholar]
  14. SADANA J. C., MOREY A. V. The purification of hydrogenase of Desulfovibrio desulfuricans. Biochim Biophys Acta. 1959 Apr;32:592–593. doi: 10.1016/0006-3002(59)90655-9. [DOI] [PubMed] [Google Scholar]
  15. SENEZ J. C. Consommation de l'hydrogène moléculaire par les suspensions nonproliférantes et par les extraits cellulaires de Desulfovibrio (Sporovibrio) desulfuricans. Bull Soc Chim Biol (Paris) 1955;37(11):1135–1146. [PubMed] [Google Scholar]

Articles from Journal of Bacteriology are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES