Abstract
Shapira, Jacob (Consolidated Veterans Administration Hospital, Little Rock, Ark.), Lois Lowden, and Ralph Hale. Inhibition of a thymine-deficient mutant of Escherichia coli by 5-substituted uracils. J. Bacteriol. 83:919–923. 1962.—Small inocula of well-washed cells of a thymine-requiring mutant of Escherichia coli were incubated in a thymine-containing glucose-salts medium with a variety of 5-substituted pyrimidines and pyrimidine ribosides. After a lag phase, the turbidity of the cultures increased appreciably which, in the case of 5-ethyluracil and 5-ethyluridine, was primarily due to an elongation of the cells. 5-Ethyluracil at low thymine concentrations increased the lag phase and decreased the rate and final amount of growth. At high thymine concentrations, it had less effect on the final turbidity of the cultures. The inhibition index for this compound was relatively constant, suggesting competitive inhibition.
Several other pyrimidine analogues inhibited growth. The nucleosides of 5-bromouracil and 5-aminouracil were no more effective than the free bases. The ribosides of 5-ethyluracil and 5-butyluracil were appreciably more inhibitory than the free bases and were the most potent compounds tested. It is likely that the inhibition of growth is a reflection of the effect of these compounds on ribonucleic acid synthesis by the cells.
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Selected References
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- BARNER H. D., COHEN S. S. The induction of thymine synthesis by T2 infection of a thymine requiring mutant of Escherichia coli. J Bacteriol. 1954 Jul;68(1):80–88. doi: 10.1128/jb.68.1.80-88.1954. [DOI] [PMC free article] [PubMed] [Google Scholar]
- COHEN S. S., ARBOGAST R. Chemical studies in host-virus interactions; a comparison of some properties of three mutant pairs of bacterial viruses, T2r and T2r, T4r and T4r, T6r and T6r. J Exp Med. 1950 Jun 1;91(6):619–636. doi: 10.1084/jem.91.6.619. [DOI] [PMC free article] [PubMed] [Google Scholar]
- COHEN S. S., BARNER H. D. Studies on the induction of thymine deficiency and on the effects of thymine and thymidine analogues in Escherichia coli. J Bacteriol. 1956 May;71(5):588–597. doi: 10.1128/jb.71.5.588-597.1956. [DOI] [PMC free article] [PubMed] [Google Scholar]
- DUNN D. B., SMITH J. D., ZAMENHOF S., GRIBOFF G. Incorporation of halogenated pyrimidines into the deoxyribonucleic acids of Bacterium coli and its bacteriophages. Nature. 1954 Aug 14;174(4424):305–307. [PubMed] [Google Scholar]
- FUKUHARA T. K., VISSER D. W. Pyrimidine nucleoside antagonists. J Biol Chem. 1951 May;190(1):95–101. [PubMed] [Google Scholar]
- HANAWALT P. C., MAALOE O., CUMMINGS D. J., SCHAECHTER M. The normal DNA replication cycle. II. J Mol Biol. 1961 Apr;3:156–165. doi: 10.1016/s0022-2836(61)80042-9. [DOI] [PubMed] [Google Scholar]
- MAALOE O., HANAWALT P. C. Thymine deficiency and the normal DNA replication cycle. I. J Mol Biol. 1961 Apr;3:144–155. doi: 10.1016/s0022-2836(61)80041-7. [DOI] [PubMed] [Google Scholar]
- ZAMENHOF S., DE GIOVANNI R., RICH K. Escherichia coli containing unnatural pyrimidines in its deoxyribonucleic acid. J Bacteriol. 1956 Jan;71(1):60–69. doi: 10.1128/jb.71.1.60-69.1956. [DOI] [PMC free article] [PubMed] [Google Scholar]