Abstract
Kelton, William H. (University of Massachusetts, Amherst). Synchronized division of avian pleuropneumonia-like organisms. J. Bacteriol. 83:948–955. 1962.—Evidence indicates that phosphate-buffered 0.85% saline (pH 7.0) is toxic when used for diluting cultures of pleuropneumonia-like organisms (PPLO). A technique for picking single PPLO colonies and streaking on agar medium is described.
Growth curves of eight strains of PPLO of avian origin are described. Strain S-6 showed a typical growth curve. The other strains presented the lag and logarithmic phases of growth, followed by a rapid decline in viable numbers and peaks of growth later. Additional studies showed that growth was logarithmic in young cultures of all strains.
Synchronization of division of two avian strains of PPLO appeared to have been induced by starvation for horse serum. Studies of small populations of PPLO were also made. The results of these experiments, along with the logarithmic growth, favor binary fission as the mode of reproduction.
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Selected References
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- EDWARD D. G., KANAREK A. D. Organisms of the pleuropneumonia group of avian origin: their classification into species. Ann N Y Acad Sci. 1960 Jan 15;79:696–702. doi: 10.1111/j.1749-6632.1960.tb42744.x. [DOI] [PubMed] [Google Scholar]
- EDWARDS G. A., FOGH J. Fine structure of pleuropneumonia-like organisms in pure culture and in infected tissue culture cells. J Bacteriol. 1960 Feb;79:267–276. doi: 10.1128/jb.79.2.267-276.1960. [DOI] [PMC free article] [PubMed] [Google Scholar]
- FREUNDT E. A. Morphological studies of the peripneumonia organism (Micromyces peripneumoniae bovis). Acta Pathol Microbiol Scand. 1952;31(4):508–529. doi: 10.1111/j.1699-0463.1952.tb00220.x. [DOI] [PubMed] [Google Scholar]
- KELLER R., MORTON H. E. The growth of pleuropneumonia-like organisms of human origin: cultivation in the developing chick embryo and an in vitro growth cycle. J Bacteriol. 1954 Feb;67(2):129–134. doi: 10.1128/jb.67.2.129-134.1954. [DOI] [PMC free article] [PubMed] [Google Scholar]
- KELTON W. H. Growth-curve studies of pleuropneumonialike organisms. Ann N Y Acad Sci. 1960 Jan 15;79:422–429. doi: 10.1111/j.1749-6632.1960.tb42707.x. [DOI] [PubMed] [Google Scholar]
- LIEBERMEISTER K. Morphology of the PPLO and L forms of Proteus. Ann N Y Acad Sci. 1960 Jan 15;79:326–343. doi: 10.1111/j.1749-6632.1960.tb42694.x. [DOI] [PubMed] [Google Scholar]
- SMITH P. F. Quantitative measurement of the growth of pleuropneumonia-like organisms. Appl Microbiol. 1956 Sep;4(5):254–259. doi: 10.1128/am.4.5.254-259.1956. [DOI] [PMC free article] [PubMed] [Google Scholar]
- SMITH P. F., SASAKI S. Stability of pleuropneumonialike organisms to some physical factors. Appl Microbiol. 1958 May;6(3):184–189. doi: 10.1128/am.6.3.184-189.1958. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Smith W. E., Hillier J., Mudd S. Electron Micrograph Studies of Two Strains of Pleuropneumonia-like (L) Organisms of Human Derivation. J Bacteriol. 1948 Nov;56(5):589–601. doi: 10.1128/jb.56.5.589-601.1948. [DOI] [PMC free article] [PubMed] [Google Scholar]
- TULASNE R., BRINGMANN G. Données nouvelles apportées par la microscopie électronique sur la morphologie des formes L des bactéries. Rev Immunol Ther Antimicrob. 1952;16(4-5):325–330. [PubMed] [Google Scholar]
- YAMAMOTO R., ADLER H. E. Characterization of pleuropneumonia-like organisms of avian origin. I. Antigenic analysis of seven strains and their comparative pathogenicity for birds. J Infect Dis. 1958 Mar-Apr;102(2):143–152. doi: 10.1093/infdis/102.2.143. [DOI] [PubMed] [Google Scholar]