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. 1962 Jun;83(6):1224–1230. doi: 10.1128/jb.83.6.1224-1230.1962

NITROGENOUS COMPOUNDS IN GERMINATION AND POSTGERMINATIVE DEVELOPMENT OF BACILLUS MEGATERIUM SPORES

Hillel S Levinson 1, Mildred T Hyatt 1
PMCID: PMC279438  PMID: 14464681

Abstract

Levinson, Hillel S. (Quartermaster Research and Engineering Center, Natick, Mass.) and Mildred T. Hyatt. Nitrogenous compounds in germination and postgerminative development of Bacillus megaterium spores. J. Bacteriol. 83:1224–1230. 1962.—Of the 48 inorganic and organic nitrogenous compounds tested, only l-alanine, glucosamine, and N-acetylglucosamine promote germination of unheated spores of Bacillus megaterium. Heated spores also germinate in nitrate, nitrite, l-cysteine, dl-isoleucine, l-leucine, dl-methionine, dl-norleucine, l-proline and l-valine.

A source of nitrogen is required for postgerminative development, but nitrogenous compounds which effect germination do not necessarily support postgerminative development. Nitrogenous compounds which support postgerminative development include (NH4)2SO4, nitrates, d-alanine, l-alanine, l-arginine, l-asparagine, l-aspartic and l-glutamic acids, glutamine, l-proline, adenine, adenosine, and guanosine. Oxygen consumption rates during postgerminative development are different with different nitrogen sources, and these rates, in general, reflect the extent of postgerminative development. Utilization of amino and ammonium nitrogen during postgerminative development was followed, and the concentration requirements were determined (ca 10 mm for at least one cell division of 5 × 108 spores). Inhibitor studies on postgerminative development are included.

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Selected References

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  1. HACHISUKA Y., ASANO N., KATO N., OKAJIMA M., KITAORI M., KUNO T. Studies on spore germination. I. Effect of nitrogen sources on spore germination. J Bacteriol. 1955 Apr;69(4):399–406. doi: 10.1128/jb.69.4.399-406.1955. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. HILLS G. M. Chemical factors in the germination of spore-bearing aerobes: observations on the influence of species, strain and conditions of growth. J Gen Microbiol. 1950 Jan;4(1):38–47. doi: 10.1099/00221287-4-1-38. [DOI] [PubMed] [Google Scholar]
  3. HYATT M. T., LEVINSON H. S. Conditions affecting Bacillus megaterium spore germination in glucose or various nitrogenous compounds. J Bacteriol. 1962 Jun;83:1231–1237. doi: 10.1128/jb.83.6.1231-1237.1962. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. HYATT M. T., LEVINSON H. S. Correlation of respiratory activity with phases of spore germination and growth in Bacillus megaterium as influenced by manganese and L-alanine. J Bacteriol. 1956 Aug;72(2):176–183. doi: 10.1128/jb.72.2.176-183.1956. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. HYATT M. T., LEVINSON H. S. Interaction of heat, glucose, L-alanine, and potassium nitrate in spore germination of Bacillus megaterium. J Bacteriol. 1961 Feb;81:204–211. doi: 10.1128/jb.81.2.204-211.1961. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. HYATT M. T., LEVINSON H. S. Sulfure requirement for postgerminative development of Bacillus megaterium spores. J Bacteriol. 1957 Jul;74(1):87–93. doi: 10.1128/jb.74.1.87-93.1957. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. HYATT M. T., LEVINSON H. S. Utilization of phosphates in the postgerminative development of spores of Bacillus megaterium. J Bacteriol. 1959 Apr;77(4):487–496. doi: 10.1128/jb.77.4.487-496.1959. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Knaysi G. Investigation of the Existence and Nature of Reserve Material in the Endospore of a Strain of Bacillus mycoides by an Indirect Method. J Bacteriol. 1945 Jun;49(6):617–622. doi: 10.1128/jb.49.6.617-622.1945. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. LEVINSON H. S., HYATT M. T. The stimulation of germination and respiration of Bacillus megaterium spores by mangasese, L-alanine and heat. J Bacteriol. 1955 Oct;70(4):368–374. doi: 10.1128/jb.70.4.368-374.1955. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. LEVINSON H. S., SEVAG M. G. Stimulation of germination and respiration of the spores of Bacillus megatherium by manganese and monovalent anions. J Gen Physiol. 1953 May;36(5):617–629. doi: 10.1085/jgp.36.5.617. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. POWELL J. F. The sporulation and germination of a strain of Bacillus megatherium. J Gen Microbiol. 1951 Nov;5(5 Suppl):993–1000. doi: 10.1099/00221287-5-5-993. [DOI] [PubMed] [Google Scholar]
  12. Rode L. J., Foster J. W. MECHANICAL GERMINATION OF BACTERIAL SPORES. Proc Natl Acad Sci U S A. 1960 Jan;46(1):118–128. doi: 10.1073/pnas.46.1.118. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. STRANGE R. E., POWELL J. F. Hexosamine-containing peptides in spores of Bacillus subtilis, B. megatherium and B. cereus. Biochem J. 1954 Sep;58(1):80–85. doi: 10.1042/bj0580080. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. WOESE C. R., MOROWITZ H. J., HUTCHISON C. A., 3rd Analysis of action of L-alanine analogues in spore germination. J Bacteriol. 1958 Dec;76(6):578–588. doi: 10.1128/jb.76.6.578-588.1958. [DOI] [PMC free article] [PubMed] [Google Scholar]

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