Abstract
Hyatt, Mildred T. (Quartermaster Research and Engineering Center, Natick, Mass.) and Hillel S. Levinson. Conditions affecting Bacillus megaterium spore germination in glucose or various nitrogenous compounds. J. Bacteriol. 83:1231–1237. 1962.—The possibility that there is more than one metabolic pathway for triggering germination of Bacillus megaterium spores was investigated. Spores were germinated in seven different “physiological germinants” under varying conditions of concentration, pH, combinations of germinants, temperature before and during germination, and chemical inhibition. l-Alanine and l-valine appear to induce germination via the same metabolic pathway (same inhibitors are effective, similar germination rate and temperature requirements); and glucose and glucosamine also appear to act similarly, but by a different pathway than l-alanine and l-valine. The other germinants, l-leucine, l-proline, and KNO3, do not correspond in all respects either to the glucose-glucosamine or to the alanine-valine pair in response to the different germination conditions. It is concluded that B. megaterium spore germination occurs via more than one pathway.
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Selected References
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- FALCONE G., SALVATORE G., COVELLI I. Mechanism of induction of spore germination in Bacillus subtilis by L-alanine and hydrogen peroxide. Biochim Biophys Acta. 1959 Dec;36:390–397. doi: 10.1016/0006-3002(59)90181-7. [DOI] [PubMed] [Google Scholar]
- HILLS G. M. Chemical factors in the germination of spore-bearing aerobes: observations on the influence of species, strain and conditions of growth. J Gen Microbiol. 1950 Jan;4(1):38–47. doi: 10.1099/00221287-4-1-38. [DOI] [PubMed] [Google Scholar]
- HYATT M. T., LEVINSON H. S. Interaction of heat, glucose, L-alanine, and potassium nitrate in spore germination of Bacillus megaterium. J Bacteriol. 1961 Feb;81:204–211. doi: 10.1128/jb.81.2.204-211.1961. [DOI] [PMC free article] [PubMed] [Google Scholar]
- LEVINSON H. S., HYATT M. T. Nitrogenous compounds in germination and postgerminative development of Bacillus megaterium spores. J Bacteriol. 1962 Jun;83:1224–1230. doi: 10.1128/jb.83.6.1224-1230.1962. [DOI] [PMC free article] [PubMed] [Google Scholar]
- LEVINSON H. S., SEVAG M. G. Stimulation of germination and respiration of the spores of Bacillus megatherium by manganese and monovalent anions. J Gen Physiol. 1953 May;36(5):617–629. doi: 10.1085/jgp.36.5.617. [DOI] [PMC free article] [PubMed] [Google Scholar]
- O'CONNOR R., HALVORSON H. Intermediate metabolism of aerobic spores. IV. Alanine deamination during the germination of spores of Bacillus cereus. J Bacteriol. 1959 Dec;78:844–851. doi: 10.1128/jb.78.6.844-851.1959. [DOI] [PMC free article] [PubMed] [Google Scholar]
- POWELL J. F. The sporulation and germination of a strain of Bacillus megatherium. J Gen Microbiol. 1951 Nov;5(5 Suppl):993–1000. doi: 10.1099/00221287-5-5-993. [DOI] [PubMed] [Google Scholar]
- PULVERTAFT R. J. V., HAYNES J. A. Adenosine and spore germination; phase-contrast studies. J Gen Microbiol. 1951 Oct;5(4):657–663. doi: 10.1099/00221287-5-4-657. [DOI] [PubMed] [Google Scholar]
- RIEMANN H., ORDAL Z. J. Germination of bacterial endospores with calcium and dipicolinic acid. Science. 1961 May 26;133(3465):1703–1704. doi: 10.1126/science.133.3465.1703. [DOI] [PubMed] [Google Scholar]
- RODE L. J., FOSTER J. W. The action of surfactants on bacterial spores. Arch Mikrobiol. 1960;36:67–94. doi: 10.1007/BF00405943. [DOI] [PubMed] [Google Scholar]
- Rode L. J., Foster J. W. MECHANICAL GERMINATION OF BACTERIAL SPORES. Proc Natl Acad Sci U S A. 1960 Jan;46(1):118–128. doi: 10.1073/pnas.46.1.118. [DOI] [PMC free article] [PubMed] [Google Scholar]