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. 1988 Jan;85(1):6–10. doi: 10.1073/pnas.85.1.6

Alternative transcription and splicing of the human porphobilinogen deaminase gene result either in tissue-specific or in housekeeping expression.

S Chretien 1, A Dubart 1, D Beaupain 1, N Raich 1, B Grandchamp 1, J Rosa 1, M Goossens 1, P H Romeo 1
PMCID: PMC279470  PMID: 3422427

Abstract

Porphobilinogen deaminase [PBGD; porphobilinogen ammonia-lyase (polymerizing), EC 4.3.1.8] is a cytosolic enzyme involved in the heme biosynthetic pathway. Two isoforms of PBGD, encoded by two mRNAs differing solely in their 5' end, are known: one is found in all cells and the other is present only in erythroid cells. We have previously shown that the human PBGD is encoded by a single gene and have now cloned and characterized this gene, which is split into 15 exons spread over 10 kilobases of DNA. We demonstrate that the two mRNAs arise from two overlapping transcription units. The first one (upstream) is active in all tissues and its promoter has some of the structural features of a housekeeping promoter; the second, located 3 kilobases downstream, is active only in erythroid cells and its promoter displays structural homologies with the beta-globin gene promoters.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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