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. Author manuscript; available in PMC: 2009 Dec 16.
Published in final edited form as: Steroids. 2006 Dec 13;72(2):171–179. doi: 10.1016/j.steroids.2006.11.005

Fig. 1.

Fig. 1

Activation of AKT by increased phosphorylation in the thyroids of TRβPV/PV mice in an age-dependent manner. (A). Thyroid extracts (50 μg) of TRβPV/PV mice and aged-matched wild-type mice were prepared and analyzed for total AKT, phosphorylated AKT (p-AKT), and protein disulfide isomerase (loading control) by Western blot analysis. (B). Densitometric analysis compared p-AKT to total AKT using a normalized ratio of TRβPV/PV mice (p-AKT/AKT)/wild-type mice (p-AKT/AKT) at each respective age [30].