Skip to main content
NCBI home page
Proceedings of the National Academy of Sciences of the United States of America logoLink to Proceedings of the National Academy of Sciences of the United States of America
. 1988 Jan;85(2):627–631. doi: 10.1073/pnas.85.2.627

Autoradiographic localization of supraspinal kappa-opioid receptors with [125I-Tyr1, D-Pro10]dynorphin A-(1-11).

C Jomary 1, J E Gairin 1, J Cros 1, J C Meunier 1
PMCID: PMC279604  PMID: 2893376

Abstract

[125I-Tyr1, D-Pro10]dynorphin A-(1-11) (125I-DP-DYN), an opioid peptide analogue that has previously been shown to be kappa selective, displays specific, saturable, and high-affinity (Kd = 0.3 nM) binding in slide-mounted sections from nerve tissue. We have used 125I-DPDYN to autoradiographically visualize supraspinal kappa-opioid receptor sites in rats, guinea pigs, and rabbits. The autoradiographic dispositions of 125I-DPDYN in sections from cerebellum are clearly different in guinea pig and rabbit, suggesting that kappa receptors have different functions in this organ of the two species. Autoradiograms from 125I-DPDYN-labeled brain sections also reveal major species differences, in particular in thalamus, which is densely labeled in rabbit and considerably less so in rat and guinea pig. The data show that 125I-DPDYN is a useful probe to visualize kappa-opioid receptor sites in nerve tissue sections directly and rapidly.

Full text

PDF
627

Images in this article

629Image
on p.629
630Image
on p.630

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Akiyama K., Gee K. W., Mosberg H. I., Hruby V. J., Yamamura H. I. Characterization of [3H][2-D-penicillamine, 5-D-penicillamine]-enkephalin binding to delta opiate receptors in the rat brain and neuroblastoma--glioma hybrid cell line (NG 108-15). Proc Natl Acad Sci U S A. 1985 Apr;82(8):2543–2547. doi: 10.1073/pnas.82.8.2543. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Atweh S. F., Kuhar M. J. Autoradiographic localization of opiate receptors in rat brain. I. Spinal cord and lower medulla. Brain Res. 1977 Mar 18;124(1):53–67. doi: 10.1016/0006-8993(77)90863-0. [DOI] [PubMed] [Google Scholar]
  3. Atweh S. F., Kuhar M. J. Autoradiographic localization of opiate receptors in rat brain. II. The brain stem. Brain Res. 1977 Jun 24;129(1):1–12. doi: 10.1016/0006-8993(77)90965-9. [DOI] [PubMed] [Google Scholar]
  4. Atweh S. F., Kuhar M. J. Autoradiographic localization of opiate receptors in rat brain. III. The telencephalon. Brain Res. 1977 Oct 14;134(3):393–405. doi: 10.1016/0006-8993(77)90817-4. [DOI] [PubMed] [Google Scholar]
  5. Bradford M. M. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal Biochem. 1976 May 7;72:248–254. doi: 10.1016/0003-2697(76)90527-3. [DOI] [PubMed] [Google Scholar]
  6. Chang K. J., Cuatrecasas P. Multiple opiate receptors. Enkephalins and morphine bind to receptors of different specificity. J Biol Chem. 1979 Apr 25;254(8):2610–2618. [PubMed] [Google Scholar]
  7. Chang K. J., Hazum E., Cuatrecasas P. Novel opiate binding sites selective for benzomorphan drugs. Proc Natl Acad Sci U S A. 1981 Jul;78(7):4141–4145. doi: 10.1073/pnas.78.7.4141. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Foote R. W., Maurer R. Kappa opiate binding sites in the substantia nigra and bulbus olfactorius of the guinea pig as shown by in vitro autoradiography. Life Sci. 1983;33 (Suppl 1):243–246. doi: 10.1016/0024-3205(83)90488-5. [DOI] [PubMed] [Google Scholar]
  9. Gairin J. E., Gouarderes C., Mazarguil H., Alvinerie P., Cros J. [D-Pro10]Dynorphin-(1-11) is a highly potent and selective ligand for kappa opioid receptors. Eur J Pharmacol. 1984 Nov 13;106(2):457–458. doi: 10.1016/0014-2999(84)90741-6. [DOI] [PubMed] [Google Scholar]
  10. Gairin J. E., Jomary C., Pradayrol L., Cros J., Meunier J. C. 125I-DPDYN, monoiodo[D-Pro10]dynorphin(1-11): a highly radioactive and selective probe for the study of kappa opioid receptors. Biochem Biophys Res Commun. 1986 Feb 13;134(3):1142–1150. doi: 10.1016/0006-291x(86)90370-0. [DOI] [PubMed] [Google Scholar]
  11. Goodman R. R., Snyder S. H. Kappa opiate receptors localized by autoradiography to deep layers of cerebral cortex: relation to sedative effects. Proc Natl Acad Sci U S A. 1982 Sep;79(18):5703–5707. doi: 10.1073/pnas.79.18.5703. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Goodman R. R., Snyder S. H., Kuhar M. J., Young W. S., 3rd Differentiation of delta and mu opiate receptor localizations by light microscopic autoradiography. Proc Natl Acad Sci U S A. 1980 Oct;77(10):6239–6243. doi: 10.1073/pnas.77.10.6239. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Gulya K., Gehlert D. R., Wamsley J. K., Mosberg H., Hruby V. J., Yamamura H. I. Light microscopic autoradiographic localization of delta opioid receptors in the rat brain using a highly selective bis-penicillamine cyclic enkephalin analog. J Pharmacol Exp Ther. 1986 Aug;238(2):720–726. [PubMed] [Google Scholar]
  14. Kosterlitz H. W., Lord J. A., Paterson S. J., Waterfield A. A. Effects of changes in the structure of enkephalins and of narcotic analgesic drugs on their interactions with mu- and delta-receptors. Br J Pharmacol. 1980 Feb;68(2):333–342. doi: 10.1111/j.1476-5381.1980.tb10422.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Kosterlitz H. W., Paterson S. J., Robson L. E. Characterization of the kappa-subtype of the opiate receptor in the guinea-pig brain. Br J Pharmacol. 1981 Aug;73(4):939–949. doi: 10.1111/j.1476-5381.1981.tb08749.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Lord J. A., Waterfield A. A., Hughes J., Kosterlitz H. W. Endogenous opioid peptides: multiple agonists and receptors. Nature. 1977 Jun 9;267(5611):495–499. doi: 10.1038/267495a0. [DOI] [PubMed] [Google Scholar]
  17. Martin W. R., Eades C. G., Thompson J. A., Huppler R. E., Gilbert P. E. The effects of morphine- and nalorphine- like drugs in the nondependent and morphine-dependent chronic spinal dog. J Pharmacol Exp Ther. 1976 Jun;197(3):517–532. [PubMed] [Google Scholar]
  18. Meunier J. C., Kouakou Y., Puget A., Moisand C. Multiple opiate binding sites in the central nervous system of the rabbit. Large predominance of a mu subtype in the cerebellum and characterization of a kappa subtype in the thalamus. Mol Pharmacol. 1983 Jul;24(1):23–29. [PubMed] [Google Scholar]
  19. Meunier J. C. Mu and kappa opiate binding sites in the rabbit CNS. Life Sci. 1982 Sep 20;31(12-13):1327–1330. doi: 10.1016/0024-3205(82)90373-3. [DOI] [PubMed] [Google Scholar]
  20. Meunier J. C., Zajac J. M. Cerebellar opiate receptors in lagomorphs. Demonstration, characterization and regional distribution. Brain Res. 1979 May 25;168(2):311–321. doi: 10.1016/0006-8993(79)90172-0. [DOI] [PubMed] [Google Scholar]
  21. Morris B. J., Herz A. Autoradiographic localization in rat brain of kappa opiate binding sites labelled by [3H]bremazocine. Neuroscience. 1986 Nov;19(3):839–846. doi: 10.1016/0306-4522(86)90302-7. [DOI] [PubMed] [Google Scholar]
  22. Pert C. B., Kuhar M. J., Snyder S. H. Opiate receptor: autoradiographic localization in rat brain. Proc Natl Acad Sci U S A. 1976 Oct;73(10):3729–3733. doi: 10.1073/pnas.73.10.3729. [DOI] [PMC free article] [PubMed] [Google Scholar]
  23. Pfeiffer A., Brantl V., Herz A., Emrich H. M. Psychotomimesis mediated by kappa opiate receptors. Science. 1986 Aug 15;233(4765):774–776. doi: 10.1126/science.3016896. [DOI] [PubMed] [Google Scholar]
  24. Quirion R., Zajac J. M., Morgat J. L., Roques B. P. Autoradiographic distribution of mu and delta opiate receptors in rat brain using highly selective ligands. Life Sci. 1983;33 (Suppl 1):227–230. doi: 10.1016/0024-3205(83)90484-8. [DOI] [PubMed] [Google Scholar]
  25. Robson L. E., Foote R. W., Maurer R., Kosterlitz H. W. Opioid binding sites of the kappa-type in guinea-pig cerebellum. Neuroscience. 1984 Jun;12(2):621–627. doi: 10.1016/0306-4522(84)90077-0. [DOI] [PubMed] [Google Scholar]
  26. Robson L. E., Gillan M. G., Kosterlitz H. W. Species differences in the concentrations and distributions of opioid binding sites. Eur J Pharmacol. 1985 May 28;112(1):65–71. doi: 10.1016/0014-2999(85)90239-0. [DOI] [PubMed] [Google Scholar]
  27. Roemer D., Buescher H. H., Hill R. C., Pless J., Bauer W., Cardinaux F., Closse A., Hauser D., Huguenin R. A synthetic enkephalin analogue with prolonged parenteral and oral analgesic activity. Nature. 1977 Aug 11;268(5620):547–549. doi: 10.1038/268547a0. [DOI] [PubMed] [Google Scholar]
  28. Unnerstall J. R., Niehoff D. L., Kuhar M. J., Palacios J. M. Quantitative receptor autoradiography using [3H]ultrofilm: application to multiple benzodiazepine receptors. J Neurosci Methods. 1982 Jul;6(1-2):59–73. doi: 10.1016/0165-0270(82)90016-4. [DOI] [PubMed] [Google Scholar]
  29. Yaksh T. L., Noueihed R. The physiology and pharmacology of spinal opiates. Annu Rev Pharmacol Toxicol. 1985;25:433–462. doi: 10.1146/annurev.pa.25.040185.002245. [DOI] [PubMed] [Google Scholar]
  30. Young W. S., 3rd, Kuhar M. J. A new method for receptor autoradiography: [3H]opioid receptors in rat brain. Brain Res. 1979 Dec 28;179(2):255–270. doi: 10.1016/0006-8993(79)90442-6. [DOI] [PubMed] [Google Scholar]
  31. Zajac J. M., Gacel G., Petit F., Dodey P., Rossignol P., Roques B. P. Deltakephalin, Tyr-D-Thr-Gly-Phe-Leu-Thr: a new highly potent and fully specific agonist for opiate delta-receptors. Biochem Biophys Res Commun. 1983 Mar 16;111(2):390–397. doi: 10.1016/0006-291x(83)90318-2. [DOI] [PubMed] [Google Scholar]
  32. Zajac J. M., Meunier J. C. Opiate receptor sites in the rabbit cerebellum: autoradiographic distribution. J Recept Res. 1980;1(3):403–413. doi: 10.3109/10799898009038790. [DOI] [PubMed] [Google Scholar]

Articles from Proceedings of the National Academy of Sciences of the United States of America are provided here courtesy of National Academy of Sciences

RESOURCES