Table 1.
GTPase activity (GTP min−1 FtsZ−1) |
|||
---|---|---|---|
buffer | WT FtsZ | FtsZ-F268C | FtsZ-F268C, labeled |
100mM KAc | 6.1 | 4.7 | 4.5 |
500 mM KAc | 4.1 | 3.2 | 0.6 |
100mM RbCl | 3.3 | 3.4 | 1.8 |
500mM RbCl | 2.1 | 2.4 | 0.6 |
All buffers contained 5 mM MgAc and are at pH 7.7. Measurements were taken in a thermostatically controlled cell at 25 °C.