Skip to main content
NCBI home page
Proceedings of the National Academy of Sciences of the United States of America logoLink to Proceedings of the National Academy of Sciences of the United States of America
. 1988 Feb;85(4):1005–1009. doi: 10.1073/pnas.85.4.1005

Isolation and expression of rat 1,25-dihydroxyvitamin D3 receptor cDNA.

J K Burmester 1, N Maeda 1, H F DeLuca 1
PMCID: PMC279689  PMID: 2829212

Abstract

The cDNA for the 1,25-dihydroxyvitamin D3 receptor has been isolated by screening a rat kidney lambda gt11 expression library with a mixture of three previously identified anti-receptor monoclonal antibodies. Protein produced from this cDNA reacts with each of the three monoclonal antibodies but does not react with an antibody specific for the porcine receptor or an irrelevant antibody. In addition, the expressed protein specifically binds 1,25-dihydroxyvitamin D3 but does not bind estradiol, progesterone, testosterone, cortisol, or aldosterone. The nucleotide sequence for this cDNA has been determined and used to deduce the amino acid sequence of the protein. This amino acid sequence contains a region that matches perfectly the partial amino acid sequence determined for the receptor isolated from porcine intestine. This amino acid sequence also closely matches the reported amino acid sequence of the putative DNA-binding finger of the avian 1,25-dihydroxyvitamin D3 receptor.

Full text

PDF
1005

Images in this article

1006Image
on p.1006

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Dame M. C., Pierce E. A., Prahl J. M., Hayes C. E., DeLuca H. F. Monoclonal antibodies to the porcine intestinal receptor for 1,25-dihydroxyvitamin D3: interaction with distinct receptor domains. Biochemistry. 1986 Aug 12;25(16):4523–4534. doi: 10.1021/bi00364a011. [DOI] [PubMed] [Google Scholar]
  2. DeLuca H. F., Schnoes H. K. Vitamin D: recent advances. Annu Rev Biochem. 1983;52:411–439. doi: 10.1146/annurev.bi.52.070183.002211. [DOI] [PubMed] [Google Scholar]
  3. Eil C., Liberman U. A., Rosen J. F., Marx S. J. A cellular defect in hereditary vitamin-D-dependent rickets type II: defective nuclear uptake of 1,25-dihydroxyvitamin D in cultured skin fibroblasts. N Engl J Med. 1981 Jun 25;304(26):1588–1591. doi: 10.1056/NEJM198106253042608. [DOI] [PubMed] [Google Scholar]
  4. Litman R. M. A deoxyribonucleic acid polymerase from Micrococcus luteus (Micrococcus lysodeikticus) isolated on deoxyribonucleic acid-cellulose. J Biol Chem. 1968 Dec 10;243(23):6222–6233. [PubMed] [Google Scholar]
  5. Mangelsdorf D. J., Pike J. W., Haussler M. R. Avian and mammalian receptors for 1,25-dihydroxyvitamin D3: in vitro translation to characterize size and hormone-dependent regulation. Proc Natl Acad Sci U S A. 1987 Jan;84(2):354–358. doi: 10.1073/pnas.84.2.354. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Maxam A. M., Gilbert W. A new method for sequencing DNA. Proc Natl Acad Sci U S A. 1977 Feb;74(2):560–564. doi: 10.1073/pnas.74.2.560. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. McDonnell D. P., Mangelsdorf D. J., Pike J. W., Haussler M. R., O'Malley B. W. Molecular cloning of complementary DNA encoding the avian receptor for vitamin D. Science. 1987 Mar 6;235(4793):1214–1217. doi: 10.1126/science.3029866. [DOI] [PubMed] [Google Scholar]
  8. Napoli J. L., Mellon W. S., Fivizzani M. A., Schnoes H. K., DeLuca H. F. Direct chemical synthesis of 1 alpha,25-dihydroxy[26,27-3H]vitamin D3 with high specific activity: its use in receptor studies. Biochemistry. 1980 May 27;19(11):2515–2521. doi: 10.1021/bi00552a033. [DOI] [PubMed] [Google Scholar]
  9. Pike J. W., Dokoh S., Haussler M. R., Liberman U. A., Marx S. J., Eil C. Vitamin D3--resistant fibroblasts have immunoassayable 1,25-dihydroxyvitamin D3 receptors. Science. 1984 May 25;224(4651):879–881. doi: 10.1126/science.6326262. [DOI] [PubMed] [Google Scholar]
  10. Pike J. W., Donaldson C. A., Marion S. L., Haussler M. R. Development of hybridomas secreting monoclonal antibodies to the chicken intestinal 1 alpha,25-dihydroxyvitamin D3 receptor. Proc Natl Acad Sci U S A. 1982 Dec;79(24):7719–7723. doi: 10.1073/pnas.79.24.7719. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Pike J. W. Monoclonal antibodies to chick intestinal receptors for 1,25-dihydroxyvitamin D3. Interaction and effects of binding on receptor function. J Biol Chem. 1984 Jan 25;259(2):1167–1173. [PubMed] [Google Scholar]
  12. Pike J. W., Sleator N. M., Haussler M. R. Chicken intestinal receptor for 1,25-dihydroxyvitamin D3. Immunologic characterization and homogeneous isolation of a 60,000-dalton protein. J Biol Chem. 1987 Jan 25;262(3):1305–1311. [PubMed] [Google Scholar]
  13. Young R. A., Davis R. W. Efficient isolation of genes by using antibody probes. Proc Natl Acad Sci U S A. 1983 Mar;80(5):1194–1198. doi: 10.1073/pnas.80.5.1194. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from Proceedings of the National Academy of Sciences of the United States of America are provided here courtesy of National Academy of Sciences

RESOURCES