TABLE 4. Binding properties of OS2 and its mutants to M-type and N-type receptors.
sPLA2s | K0.5 value | |||
---|---|---|---|---|
Rabbit M-type | Mouse M-type | Mouse N-type | HelaP4 N-type | |
nM | ||||
Venom OS2 | 0.009 | 0.07 | 0.011 | 0.02 |
rec OS2 WT | 0.009 | 0.07 | 0.011 | 0.02 |
OS2 H48Q | 0.021 | 0.19 | 0.53 | 1.2 |
OS2 D49K | 0.026 | 0.49 | 13.5 | 0.96 |
OS2 G30S | 0.035 | 0.2 | 200 | 400 |
OS2 K31L-R34S | 0.008 | 0.047 | 0.045 | 0.028 |
N-terminal chimera | 0.19 | 1.4 | >500 | >500 |
Central chimera | 0.037 | 2.2 | >300 | >300 |
C-terminal chimera | 0.024 | 0.03 | 2 | 7.5 |
venom OS1 | 0.1 | 0.15 | >1000 | >1000 |