Table 1.
summary of protein decreased in Rho0 mitochondria
| number on gels | Acc number | name | pI | Mw (kDa) seq cov. | xfold decr in Rho0 vs 143B | ||
|---|---|---|---|---|---|---|---|
| respiratory complexes subunits and assembly proteins | |||||||
| 1 | P28331 | NADH-ubiquinone oxidoreductase 75 kDa subunit | 5.42 | 77 | 42 | 6 | |
| 2 | O75489 | NADH-ubiquinone oxidoreductase 30 kDa subunit | 5.48 | 26.4 | 41 | 1.6 | |
| 3 | P19404* | NADH-ubiquinone oxidoreductase 24 kDa subunit | 5.71 | 23,75 | 30 | 1.24 | |
| 4 | O00217 | NADH-ubiquinone oxidoreductase 23 kDa subunit | 5.10 | 20.3 | 42 | 21 | |
| 5 | Q9P0J0 | NADH-ubiquinone oxidoreductase B16.6 subunit | 8.24 | 16.5 | 20 | 5.4 | |
| 6 | P47985 | Ubiquinol-cytochrome c reductase iron-sulfur subunit | 6.3 | 21.6 | 31 | 9 | |
| 7 | P31930 | Ubiquinol-cytochrome-c reductase complex core protein 1 | 5.43 | 49.1 | 46 | 1.8 | |
| 8 | P22695 | Ubiquinol-cytochrome-c reductase complex core protein 2 | 7.74 | 46.8 | 61 | 1.7 | |
| 9 | Q8TB65* | Cytochrome c oxidase subunit Va | 4.88 | 12.5 | 18 | 0.75 | |
| 10 | P10106* | Cytochrome c oxidase polypeptide Vb | 6.3 | 10.6 | 24 | 6 | |
| 11 | P25705 | ATP synthase alpha chain | 8.28 | 55.2 | 47 | 1.1 | |
| 12 | P06576 | ATP synthase beta chain | 5.0 | 51.7 | 20 | 1.1 | |
| 13 | P30049* | ATP synthase delta chain | 4.53 | 15 | 15 | 0.9 | |
| 14 | O75947* | ATP synthase D chain | 5.22/4.95 | 18/16.8 | 24 | 1.1 | |
| 15 | O75880 | SCO1 protein homolog | 8.54 | 30.3 | 20 | 3.7 | |
| mitochondrial translation apparatus | |||||||
| 16 | Q9BYD6 | Mitochondrial ribosomal protein L1 | 6.46 | 31.4 | 21 | 4.5 | |
| 17 | P52815 | 39S ribosomal protein L12 | 5.37 | 16.4 | 43 | 10 | |
| 18 | Q96GC5 | Mitochondrial ribosomal protein L48 | 7.78 | 20.6 | 36 | 4 | |
| 19 | Q9Y399 | Mitochondrial 28S ribosomal protein S2 | 7.35 | 28.3 | 41 | 20 | |
| 20 | P82650 | Mitochondrial 28S ribosomal protein S22 | 6.52 | 38.1 | 52 | 3 | |
| 21 | Q9Y3D9 | Mitochondrial ribosomal protein S23 | 8.94 | 21.7 | 55 | 2.3 | |
| 22 | Q92552 | Mitochondrial 28S ribosomal protein S27 | 5.37 | 43.7 | 27 | 2.0 | |
| 23 | P51398 | Mitochondrial 28S ribosomal protein S29 | 8.75 | 43.5 | 29 | 2.9 | |
| 24 | Q9NP92 | Mitochondrial 28S ribosomal protein S30 | 8.24 | 50.3 | 30 | 10.5 | |
| 25 | Q9UGM6 | Tryptophanyl-tRNA synthetase | 8.99/8.29 | 37.9/35.1 | 31 | 2.9 | |
| 26 | Q96Q11 | tRNA-nucleotidyltransferase 1 (CCA addition) | 6.29/6.66 | 44.9/48.0 | 19 | 5.5 | |
| mitochondrial transport systems | |||||||
| 27 | O75431 | Metaxin 2 | 5.9 | 29.7 | 35 | 2.1 | |
| 28 | P57105 | Mitochondrial outer membrane protein 25 | 5.85 | 15.9 | 59 | 2.5 | |
| 29 | Q9P129 | Calcium-binding transporter | 6.0 | 53.3 | 31 | 3.5 | |
| 30 | Q9Y5J7* | Mito. import inner mb. translocase subunit TIM9 A | 6.71 | 10.3 | 30 | 5.0 | |
| 31 | Q9NS69 | Mitochondrial import receptor subunit TOM22 | 4.27 | 15.4 | 77 | 2.3 | |
| 32 | Q9UJZ1 | stomatin-like protein 2 | 5.26 | 35.0 | 64 | 1.9 | 0,99 |
| Energy systems | |||||||
| 33 | P13804 | Electron transfer flavoprotein alpha-subunit | 7.1 | 32.9 | 52 | 2.4 | |
| 34 | P38117 | Electron transfer flavoprotein beta-subunit | 8.25 | 27.8 | 50 | 2.3 | |
| 35 | Q16134 | Electron transfer flavoprotein-ubiquinone oxidoreductase | 6.42 | 63.7 | 37 | 2.3 | |
| 36 | Q16836 | Short chain 3-hydroxyacyl-CoA dehydrogenase | 8.38 | 32.5 | 31 | 3.5 | |
| 37 | Q99714 | 3hydroxyacyl-CoA dehydrogenase type II | 7.65 | 26.9 | 55 | 2.3 | |
| 38 | P49748 | Acyl-CoA dehydrogenase, very-long-chain specific | 7.74 | 66.1 | 52 | 1.74 | |
| 39 | P36957 | Dihydrolipoamide succinyltransf. (2-oxoglutarate DH cplx) | 6.06 | 41.8 | 39 | 2.1 | |
| 40 | P09622 | Dihydrolipoamide dehydrogenase | 6.35 | 50.1 | 50 | 6.1 | |
| 41 | Q9P2R7 | Succinyl-CoA ligase [ADP-forming] beta-chain | 5.64 | 43.6 | 36 | 8.6 | |
| 42 | Q9HCC0 | Methylcrotonoyl-CoA carboxylase beta chain | 6.61 | 59.6 | 49 | 5.8 | |
| 43 | Q8WVX0 | putative enoyl-CoA hydratase | 6.22 | 32.1 | 49 | 1.7 | 0,98 |
| 44 | P78540 | arginase II | 5.42 | 36;1 | 44 | 1.9 | |
| Miscellaneous | |||||||
| 45 | O00165 | Hax-1 | 4.76 | 31.6 | 53 | 2.8 | |
| 46 | Q9NR28 | Smac protein | 4.93 | 22.1 | 27 | 1.8 | |
| 47 | Q16762 | Rhodanese | 6.11 | 29.0 | 40 | 1.8 | |
| 48 | Q6YN16 | Hydroxysteroid dehydrogenase | 6.31 | 42.5 | 41 | 4.45 | |
| 49 | Q6P587 | fum. Acetoacetate lyase domain cont. Protein | 6.0 | 21.7 | 38 | 3 | 0,85 |
| 50 | Q9BSH4 * | UPF0082 protein PRO0477 | 6.98 | 29.9 | 17 | 2.2 | 0,98 |
| 51 | Q96EH3 | Chromosome 7 open reading frame 30 | 4.87 | 21.6 | 27 | 7 | 0,98 |
*
proteins for which MS/MS data were obtained and confirmed the identification