Skip to main content
NCBI home page
Proceedings of the National Academy of Sciences of the United States of America logoLink to Proceedings of the National Academy of Sciences of the United States of America
. 1988 Mar;85(6):1792–1795. doi: 10.1073/pnas.85.6.1792

Human liver type pyruvate kinase: complete amino acid sequence and the expression in mammalian cells.

K Tani 1, H Fujii 1, S Nagata 1, S Miwa 1
PMCID: PMC279865  PMID: 3126495

Abstract

Pyruvate kinase (PK) has four isozymes (L, R, M1, M2) that are encoded by two different genes. Among these isozymes, abnormalities of liver (L)-type PK is considered to be associated with hereditary nonspherocytic hemolytic anemia in humans. We isolated and determined the full-length sequence of human L-type PK cDNA. The cDNA contains 1629 base pairs encoding 543 amino acids, 68 base pairs of 5'-noncoding sequence, and 734 base pairs of 3'-noncoding sequence. The similarity between human and rat L-type PK was 86.9% at the nucleotide sequence level and 92.4% at the amino acid sequence level. The full-length L-type PK cDNA was placed under the promoter of simian virus 40 and introduced into monkey COS cells. Human L-type PK activity was detected in the extract of COS cells by the classical PK electrophoresis method.

Full text

PDF
1792

Images in this article

1795Image
on p.1795

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Anderson W. F. Prospects for human gene therapy. Science. 1984 Oct 26;226(4673):401–409. doi: 10.1126/science.6093246. [DOI] [PubMed] [Google Scholar]
  2. Burke R. L., Tekamp-Olson P., Najarian R. The isolation, characterization, and sequence of the pyruvate kinase gene of Saccharomyces cerevisiae. J Biol Chem. 1983 Feb 25;258(4):2193–2201. [PubMed] [Google Scholar]
  3. Cognet M., Lone Y. C., Vaulont S., Kahn A., Marie J. Structure of the rat L-type pyruvate kinase gene. J Mol Biol. 1987 Jul 5;196(1):11–25. doi: 10.1016/0022-2836(87)90507-9. [DOI] [PubMed] [Google Scholar]
  4. Daar I. O., Artymiuk P. J., Phillips D. C., Maquat L. E. Human triose-phosphate isomerase deficiency: a single amino acid substitution results in a thermolabile enzyme. Proc Natl Acad Sci U S A. 1986 Oct;83(20):7903–7907. doi: 10.1073/pnas.83.20.7903. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Ibsen K. H. Interrelationships and functions of the pyruvate kinase isozymes and their variant forms: a review. Cancer Res. 1977 Feb;37(2):341–353. [PubMed] [Google Scholar]
  6. Imamura K., Tanaka T. Multimolecular forms of pyruvate kinase from rat and other mammalian tissues. I. Electrophoretic studies. J Biochem. 1972 Jun;71(6):1043–1051. doi: 10.1093/oxfordjournals.jbchem.a129852. [DOI] [PubMed] [Google Scholar]
  7. Imamura K., Tanaka T., Nishina T., Nakashima K., Miwa S. Studies on pyruvate kinase (PK) deficiency. II. Electrophoretic, kinetic and immunological studies on pyruvate kinase of erythrocytes and other tissues. J Biochem. 1973 Dec;74(6):1165–1175. doi: 10.1093/oxfordjournals.jbchem.a130344. [DOI] [PubMed] [Google Scholar]
  8. Inoue H., Noguchi T., Tanaka T. Complete amino acid sequence of rat L-type pyruvate kinase deduced from the cDNA sequence. Eur J Biochem. 1986 Jan 15;154(2):465–469. doi: 10.1111/j.1432-1033.1986.tb09420.x. [DOI] [PubMed] [Google Scholar]
  9. Kishi H., Mukai T., Hirono A., Fujii H., Miwa S., Hori K. Human aldolase A deficiency associated with a hemolytic anemia: thermolabile aldolase due to a single base mutation. Proc Natl Acad Sci U S A. 1987 Dec;84(23):8623–8627. doi: 10.1073/pnas.84.23.8623. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Lonberg N., Gilbert W. Intron/exon structure of the chicken pyruvate kinase gene. Cell. 1985 Jan;40(1):81–90. doi: 10.1016/0092-8674(85)90311-3. [DOI] [PubMed] [Google Scholar]
  11. Lonberg N., Gilbert W. Primary structure of chicken muscle pyruvate kinase mRNA. Proc Natl Acad Sci U S A. 1983 Jun;80(12):3661–3665. doi: 10.1073/pnas.80.12.3661. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Lone Y. C., Simon M. P., Kahn A., Marie J. Complete nucleotide and deduced amino acid sequences of rat L-type pyruvate kinase. FEBS Lett. 1986 Jan 20;195(1-2):97–100. doi: 10.1016/0014-5793(86)80138-7. [DOI] [PubMed] [Google Scholar]
  13. Marie J., Kahn A., Boivin P. L-type pyruvate kinase from human liver. Purification by double affinity elution, electrofocusing and immunological studies. Biochim Biophys Acta. 1976 Jul 8;438(2):393–406. doi: 10.1016/0005-2744(76)90256-4. [DOI] [PubMed] [Google Scholar]
  14. Marie J., Simon M. P., Dreyfus J. C., Kahn A. One gene, but two messenger RNAs encode liver L and red cell L' pyruvate kinase subunits. Nature. 1981 Jul 2;292(5818):70–72. doi: 10.1038/292070a0. [DOI] [PubMed] [Google Scholar]
  15. Marie J., Simon M. P., Kahn A. Cotranslation of L and L' pyruvate kinase messenger RNAs from human fetal liver. Biochim Biophys Acta. 1982 Mar 29;696(3):340–344. doi: 10.1016/0167-4781(82)90066-5. [DOI] [PubMed] [Google Scholar]
  16. Marx J. L. Gene therapy--so near and yet so far away. Science. 1986 May 16;232(4752):824–825. doi: 10.1126/science.3085217. [DOI] [PubMed] [Google Scholar]
  17. Mori N., Singer-Sam J., Lee C. Y., Riggs A. D. The nucleotide sequence of a cDNA clone containing the entire coding region for mouse X-chromosome-linked phosphoglycerate kinase. Gene. 1986;45(3):275–280. doi: 10.1016/0378-1119(86)90025-9. [DOI] [PubMed] [Google Scholar]
  18. Muirhead H., Clayden D. A., Barford D., Lorimer C. G., Fothergill-Gilmore L. A., Schiltz E., Schmitt W. The structure of cat muscle pyruvate kinase. EMBO J. 1986 Mar;5(3):475–481. doi: 10.1002/j.1460-2075.1986.tb04236.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Nagata S., Tsuchiya M., Asano S., Kaziro Y., Yamazaki T., Yamamoto O., Hirata Y., Kubota N., Oheda M., Nomura H. Molecular cloning and expression of cDNA for human granulocyte colony-stimulating factor. 1986 Jan 30-Feb 5Nature. 319(6052):415–418. doi: 10.1038/319415a0. [DOI] [PubMed] [Google Scholar]
  20. Noguchi T., Inoue H., Tanaka T. The M1- and M2-type isozymes of rat pyruvate kinase are produced from the same gene by alternative RNA splicing. J Biol Chem. 1986 Oct 15;261(29):13807–13812. [PubMed] [Google Scholar]
  21. Noguchi T., Yamada K., Inoue H., Matsuda T., Tanaka T. The L- and R-type isozymes of rat pyruvate kinase are produced from a single gene by use of different promoters. J Biol Chem. 1987 Oct 15;262(29):14366–14371. [PubMed] [Google Scholar]
  22. O'Hare K., Benoist C., Breathnach R. Transformation of mouse fibroblasts to methotrexate resistance by a recombinant plasmid expressing a prokaryotic dihydrofolate reductase. Proc Natl Acad Sci U S A. 1981 Mar;78(3):1527–1531. doi: 10.1073/pnas.78.3.1527. [DOI] [PMC free article] [PubMed] [Google Scholar]
  23. Rottmann W. H., Tolan D. R., Penhoet E. E. Complete amino acid sequence for human aldolase B derived from cDNA and genomic clones. Proc Natl Acad Sci U S A. 1984 May;81(9):2738–2742. doi: 10.1073/pnas.81.9.2738. [DOI] [PMC free article] [PubMed] [Google Scholar]
  24. Saheki S., Saheki K., Tanaka T. Peptide structures of pyruvate kinase isozymes. I. Comparison of the four pyruvate kinase isozymes of the rat. Biochim Biophys Acta. 1982 Jun 24;704(3):484–493. doi: 10.1016/0167-4838(82)90071-1. [DOI] [PubMed] [Google Scholar]
  25. Sakakibara M., Mukai T., Hori K. Nucleotide sequence of a cDNA clone for human aldolase: a messenger RNA in the liver. Biochem Biophys Res Commun. 1985 Aug 30;131(1):413–420. doi: 10.1016/0006-291x(85)91818-2. [DOI] [PubMed] [Google Scholar]
  26. Shinohara K., Miwa S., Nakashima K., Oda E., Kageoka T. A new pyruvate kinase variant (PK Osaka) demonstrated by partial purification and condensation. Am J Hum Genet. 1976 Sep;28(5):474–481. [PMC free article] [PubMed] [Google Scholar]
  27. Sorge J., Kuhl W., West C., Beutler E. Complete correction of the enzymatic defect of type I Gaucher disease fibroblasts by retroviral-mediated gene transfer. Proc Natl Acad Sci U S A. 1987 Feb;84(4):906–909. doi: 10.1073/pnas.84.4.906. [DOI] [PMC free article] [PubMed] [Google Scholar]
  28. Tani K., Fujii H., Tsutsumi H., Sukegawa J., Toyoshima K., Yoshida M. C., Noguchi T., Tanaka T., Miwa S. Human liver type pyruvate kinase: cDNA cloning and chromosomal assignment. Biochem Biophys Res Commun. 1987 Mar 13;143(2):431–438. doi: 10.1016/0006-291x(87)91372-6. [DOI] [PubMed] [Google Scholar]
  29. VALENTINE W. N., TANAKA K. R., MIWA S. A specific erythrocyte glycolytic enzyme defect (pyruvate kinase) in three subjects with congenital non-spherocytic hemolytic anemia. Trans Assoc Am Physicians. 1961;74:100–110. [PubMed] [Google Scholar]
  30. Valentine W. N., Tanaka K. R., Paglia D. E. Hemolytic anemias and erythrocyte enzymopathies. Ann Intern Med. 1985 Aug;103(2):245–257. doi: 10.7326/0003-4819-103-2-245. [DOI] [PubMed] [Google Scholar]
  31. Yoshida A., Tani K. Phosphoglycerate kinase abnormalities: functional, structural and genomic aspects. Biomed Biochim Acta. 1983;42(11-12):S263–S267. [PubMed] [Google Scholar]

Articles from Proceedings of the National Academy of Sciences of the United States of America are provided here courtesy of National Academy of Sciences

RESOURCES