Abstract
Restriction site variation in chloroplast DNA was examined in the neotropical fern genus Phanerophlebia and in selected species of the related Asiatic genus Cyrtomium and the cosmopolitan progenitor of these two, Polystichum. A total of 103 restriction site mutations was identified; these were used to construct phylogenetic networks and trees based on Wagner and Dollo parsimony and Fitch-Margoliash distance algorithms. The analyses provided evidence that Phanerophlebia did not arise from Cyrtomium. Both genera are convergent descendants from different progenitor groups in Polystichum, and Asiatic Cyrtomium is more closely related to temperate New World Polystichum than it is to neotropical Phanerophlebia. Reticulate venation, previously considered an important taxonomic character for infrageneric classification in Phanerophlebia, most likely evolved independently twice within the genus. Diploid maternal progenitors are suggested for two of four tetraploid species analyzed, and species-level distinctions for two closely related taxa of Phanerophlebia are questioned.
Keywords: restriction site variation, plant taxonomy, Polystichum, Cyrtomium
Full text
PDF
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- Clegg M. T., Rawson J. R., Thomas K. Chloroplast DNA variation in pearl millet and related species. Genetics. 1984 Mar;106(3):449–461. doi: 10.1093/genetics/106.3.449. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Nei M., Li W. H. Mathematical model for studying genetic variation in terms of restriction endonucleases. Proc Natl Acad Sci U S A. 1979 Oct;76(10):5269–5273. doi: 10.1073/pnas.76.10.5269. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Palmer J. D., Jorgensen R. A., Thompson W. F. Chloroplast DNA variation and evolution in pisum: patterns of change and phylogenetic analysis. Genetics. 1985 Jan;109(1):195–213. doi: 10.1093/genetics/109.1.195. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Palmer J. D. Physical and gene mapping of chloroplast DNA from Atriplex triangularis and Cucumis sativa. Nucleic Acids Res. 1982 Mar 11;10(5):1593–1605. doi: 10.1093/nar/10.5.1593. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Palmer J. D., Zamir D. Chloroplast DNA evolution and phylogenetic relationships in Lycopersicon. Proc Natl Acad Sci U S A. 1982 Aug;79(16):5006–5010. doi: 10.1073/pnas.79.16.5006. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Southern E. M. Detection of specific sequences among DNA fragments separated by gel electrophoresis. J Mol Biol. 1975 Nov 5;98(3):503–517. doi: 10.1016/s0022-2836(75)80083-0. [DOI] [PubMed] [Google Scholar]
- Sytsma K. J., Gottlieb L. D. Chloroplast DNA evidence for the origin of the genus Heterogaura from a species of Clarkia (Onagraceae). Proc Natl Acad Sci U S A. 1986 Aug;83(15):5554–5557. doi: 10.1073/pnas.83.15.5554. [DOI] [PMC free article] [PubMed] [Google Scholar]