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. Author manuscript; available in PMC: 2010 Nov 9.
Published in final edited form as: Biomacromolecules. 2009 Nov 9;10(11):3114–3121. doi: 10.1021/bm900846m

Figure 6.

Figure 6

(A) Chemical structure of the PEGTA20000 macromer used to encapsulation fibroblast cells using the GOX-mediated initiation system. (B) Fibroblast (NIH3T3) cells were encapsulated into a 15wt% PEGTA20000 using the GOX-mediated initiation system with 4.0 × 10−3 M glucose, 1.25 × 10−3 M Fe+2, 2.5 × 10−5 M GOX, 1mM CRGDS and 1X PBS. The cell-laden gels were incubated in DMEM glucose media without catalase for either 0, 1 or 24 hours or in DMEM glucose media supplemented with 2.0 × 10−6 M of catalase enzyme for 24 hours. The 24 hour incubations with, and without, catalase were significantly different (α = 0.01). The cell viability was quantified using a LIVE/DEAD membrane integrity assay.