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. 2009 Dec 22;53:10.3402/fnr.v53i0.2023. doi: 10.3402/fnr.v53i0.2023

Fruit and vegetable consumption close to recommendations. A partly web-based nationwide dietary survey in Swedish adults

Anna-Mari Simunaniemi 1,*, Agneta Andersson 1, Margaretha Nydahl 1
PMCID: PMC2801502  PMID: 20049176

Abstract

Background

Fruit and vegetables (F&V) are strongly associated with health. The latest Swedish national dietary survey from a decade ago showed that consumption of F&V was below recommended levels. However, current consumption in different subgroups is not well known.

Objective

To investigate the consumption of various F&V types in Swedish adults grouped according to sociodemographic factors and self-reported physical activity (PA).

Design

A cross-sectional survey using a quantitative pen-and-paper or web-based questionnaire in a population-based random sample of adults 18–84 years (final n=1,304; 51%). A self-administered 24-h recall and a food frequency questionnaire (FFQ) were used to measure F&V consumption. Data on gender, age, education level, country of birth, and PA (hours/week) were included as grouping variables. Besides descriptive data, two-sample t-tests and non-parametric tests were performed. A P-value <0.01 was regarded as significant.

Results

Mean F&V consumption based on the self-administered 24-h recall was close to the recommended five portions/day: 5.4 (99% CI 5.1–5.6) portions/day among women and 4.7 (4.4–5.0) portions/day among men (P<0.001). Also the FFQ showed that women generally consumed more F&V than men did. Consumption was lowest among respondents with ≤ 0.5 h self-reported PA/week (P≤0.001), as well as among men born in Sweden (P=0.006). F&V were consumed in almost equal amounts, and fresh F&V were most popular. Intake of berries and cooked F&V was relatively low.

Conclusion

The present study shows a relatively high F&V consumption close to the recommended five portions per day. Gender differences still exist. Also PA and country of birth were significantly associated with F&V consumption.

Keywords: fruit and vegetables, dietary survey, physical activity, questionnaire, web-based


Higher fruit and vegetables (F&V) consumption has been associated with a reduced risk for cardiovascular disease (1, 2), cancer (36) and stroke (7), and reduced mortality (8). A total of 2.7 million deaths annually are estimated to be attributable to low F&V consumption (9). Authorized bodies worldwide recommend eating at least 400 g of F&V per day (3, 10, 11), which is estimated to be equivalent to approximately five portions (12). However, the latest Swedish nationwide food consumption survey from a decade ago showed that an average F&V consumption (excluding potatoes) was around 4 times/day among women and 2.8 times/day among men (13). Another, less comprehensive survey from 2006 indicated that only 12% of women and 4% of men between 18 and 84 years consumed five or more F&V portions per day (14). However, detailed information on the different types of F&V consumed and information on respondents’ sociodemographic variables were not reported.

It has been suggested that several factors, such as gender, age, education, living conditions, and lifestyle are associated with F&V consumption. Men generally consume less F&V than women do (1518), and particularly older women have reported higher F&V consumption than younger women have (19). Further, men living on their own consume significantly less fruit than do cohabiting men (20, 21), and being married has been strongly associated with higher vegetable consumption (22). Also higher education (16, 18, 19, 2227) and physical activity (PA) (22, 24, 25, 28) have been strongly associated with higher consumption.

During recent years, imported fresh or processed fruit, fresh-cut raw salads, and other new types of F&V are being sold in increasing amounts (29). Knowledge about and understanding of what affects F&V consumption in different population groups is essential for determining whether this product development has affected F&V consumption as well as for identifying which sub-groups have the lowest consumption and should be the targets of F&V promotion projects. A decade has passed since the latest nationwide food consumption survey among Swedish adults was conducted. The present study focuses particularly on current F&V consumption in different demographic groups as well as to collect detailed information on the different types of F&V consumed. The aim of the present study was to investigate how consumption of different F&V types is related to demographic factors such as age, gender, education, and country of birth, in a randomly selected nationwide sample of Swedish adults.

Material and methods

An age-group stratified (18–24, 25–34, 35–44, 45–54, 55–64, 65–74, and 75–84 y) geographically representative random sample of 2,604 Swedish inhabitants with an equal number of women and men was selected by Swedish National Tax Agency in 2007. The respondents were divided into equally large summer and autumn groups to cover the possible seasonal variation in F&V consumption. A questionnaire enclosed with an invitation letter was sent out by mail. The respondents were also given a web-based questionnaire as an alternative measure; this was done to encourage respondents who find a traditional pen-and-paper questionnaire too time-consuming to complete the survey. As compensation, the respondents could receive a short report of the main results of the study, if they wished. A few weeks after the first circular, a reminder letter was sent to the non-responders. To increase the response rate, a second reminder was sent to all non-respondents in January 2008. For a detailed picture of the study design, see Fig. 1. The returned questionnaires were treated confidentially, the respondents gave their informed consents, and The Swedish Research Council's guidelines for good research practice (30) were followed in conducting the present study.

Fig. 1.

Fig. 1.

The study design.

Measurements

Detailed information regarding consumption of different kinds of F&V was collected using a 13-item self-administered 24-h recall and a 12-item food frequency questionnaire (FFQ). Respondents were asked to report consumed amounts either in deciliters or number of portions in the self-administered 24-h recall (e.g. ‘Did you eat apples yesterday and, if yes, how many?’). The FFQ included eight response categories for each item (‘never,’ ‘less than once/week, ‘once/week,’ ‘2–4 times/week,’ ‘5–6 times/week,’ ‘once/day,’ two times/day,’ and ‘more than twice a day’), indicating the average consumption frequencies for salad/raw and cooked vegetables as well as for fresh fruit and fruit juice (e.g. ‘How often do you usually eat fresh fruit?’).

Both the FFQ and the self-administered 24-h recall were based on a validated questionnaire where no differences were observed between the 24-h recall and one-day weighed food record of F&V (31). In addition, the FFQ showed a correlation coefficient of 0.73 for total F&V with a seven-day food record (31). The present questionnaire was also pre-tested on 20 Swedish adults, and one focus group interview (n=5) was held in which respondents discussed F&V. Minor adjustments were made on the basis of comments from the pilot tests.

Mean daily consumption of different types of fruits, vegetables, as well as total consumption (including berries and juice, excluding potatoes) on a population-level was calculated from the self-administered 24-h recall. The FFQ was used for analyzing typical consumption frequencies over a longer time period. Data on different sociodemographic variables (gender, age, household size, country of birth, and level of education) are presented in Table 1. Level of PA was measured by asking how many hours per week the respondents engage in exercise that causes them to perspire or become breathless.

Table 1.

Respondent characteristics and fruit and vegetable consumption (portions/day) according to the self-administered 24-h recall

Grouping variable Women (total n=732) Men (total n=572)


N(%) Mean 99% CI P N(%) Mean 99% CI P
All ages 723 5.4 5.1–5.6 565 4.7 4.7–5.0
18–24 y 92 (13) 5.2 4.4–6.0 nsa 51 (9) 4.2 3.1–5.4 nsa
25–34 y 108 (15) 5.1 4.4–5.8 76 (13) 4.5 3.6–5.5
35–44 y 109 (15) 4.9 4.2–5.5 69 (12) 4.7 3.7–5.7
45–54 y 112 (15) 5.4 4.7–6.1 79 (14) 4.7 3.9–5.5
55–64 y 127 (18) 5.8 5.2–6.5 92 (16) 4.5 3.9–5.1
65–74 y 110 (15) 5.6 4.9–6.2 103 (18) 5.0 4.2–5.8
75–84 y 63 (9) 5.5 4.7–6.4 95 (17) 4.8 3.9–5.6
Single household 153 (20) 5.1 4.5–5.6 nsb 120 (22) 4.7 4.0–5.3 nsb
Multi-person household 560 (20) 5.5 5.2–5.8 435 (78) 4.7 4.3–5.1
Elementary school 198 (28) 5.2 4.7–5.7 nsa 157 (28) 4.5 3.9–5.1 nsa
Medium education 253 (35) 5.1 4.7–5.6 225 (40) 4.6 4.0–5.1
University 263 (37) 5.7 5.2–6.1 174 (31) 5.0 4.5–5.6
Employed 356 (50) 5.4 5.0–5.8 nsa 281 (50) 4.5 4.1–4.9 nsa
Retired age 158 (22) 5.5 5.0–6.1 177 (32) 4.9 4.3–5.4
Other 203 (28) 5.1 4.5–5.7 101 (18) 4.9 4.0–5.7
Born in Sweden 624 (87) 5.3 5.0–5.6 nsb 497 (89) 4.5 4.2–4.8 0.006b
Born in another 94 (13) 5.7 4.8–6.5 62 (11) 6.0 4.7–7.3
Physical activity
≤0.5 h/week 221 (31) 4.6 4.1–5.1 <0.001a,c 197 (35) 4.0 3.5–4.5 0.001a, d
0.6–1.0 h/week 161 (23) 5.2 4.7–5.8 87 (15) 4.8 4.0–5.6
1.1–2.5 h/week 216 (30) 5.9 5.4–6.4 182 (32) 4.9 4.4–5.5
>2.5 h/week 114 (16) 6.0 5.3–6.7 96 (17) 5.5 4.6–6.3

Note: Groups with significantly (P≤0.01) higher consumption with bolded figures.

aOne-way ANOVA within the grouping variable.

bTwo independent samples T-test within the grouping variable.

cTukey HSD post test: ≤0.5 h vs. 1.1–2.5 h, P<0.001; ≤0.5 h vs. >2.5 h, P<0.001.

dTukey HSD post test: ≤0.5 h vs. >2.5 h, P=0.001.

Statistics

The analyses were undertaken separately for women and men as well as for different demographic groups. Means and 99% confidence intervals [99% CIs] were calculated for daily F&V consumption (portions/day). Respondents reporting unusually high consumption of >15 F&V portions/day were excluded from the analyses (five men and five women). Two-sample t-tests and one-way ANOVA were used to determine whether mean F&V consumption from the self-administered 24-h recall differed between subgroups. Where needed, differences between subcategories were analyzed using Tukey's post-hoc test. Based on normality tests and regarding the large sample size, the variable was used in its original form. Mann–Whitney test was used to analyze consumption data from the FFQ. Due to the large number of significance tests conducted, a P-value <0.01 (two-sided) was considered statistically significant throughout the study. All statistical analyses were performed using SPSS 16.0 (32).

Results

A total of 1,304 questionnaires were returned and properly filled out, giving a response rate of 51%. Approximately one-third of the respondents chose the web-based questionnaire. There were more women than men (56% vs. 44%) among the respondents. The respondents are geographically representative of the whole of Sweden. Most respondents (79%) lived with another person. Roughly one-third (37%) had a university-level education, and 49% had been employed during the past six months. About 12% of the respondents were born outside Sweden, compared to roughly 17% in the total Swedish population (33). Sociodemographic characteristics of the respondents and total F&V consumption in each subgroup are presented in Table 1.

Consumption in different demographic groups

According to the self-administered 24-h recall, total F&V consumption was 2.2 (99% CI 2.0–2.4) portions per day in the lowest quartile among women (n=187) and 1.9 (1.7–2.1) daily portions among men (n=201). The highest quartile of women (n=159) consumed 9.9 (9.3–10.4) portions on the examined day compared to 9.4 (8.4–10.5) portions among men (n=143). Mean number of total F&V portions varied from 4.9 to 5.8 among women and 4.2 to 5.0 among men in different age groups, but consumption did not differ statistically across age groups. Men born in Sweden had lower total F&V consumption than did men born in another country (P=0.006). Women with ≤0.5 h self-reported PA/week had lower F&V consumption than those reporting ≥1 h PA/week (P<0.001). Also among men, those reporting ≤0.5 h PA/week had lower F&V consumption than those reporting ≥2.5 h PA/week. For more details, see Table 1.

Consumption of different types of F&V, based on the self-administered 24-h recall, by gender and age group is presented in Table 2. Fresh fruits, as well as raw vegetables and salad, were consumed in the greatest amounts, and more by women than by men (P<0.001). Comparisons between women and men within each age group showed that 55- to 74-year-old women consumed more fruits in total (P≤0.001), and more fresh fruits (P≤0.002), than men. Women between 55 and 64 years also had higher total vegetable consumption than men in the same age group (P=0.002). Overall consumption of berries and cooked vegetables was relatively low, and no gender differences were shown.

Table 2.

Mean consumption of different fruit and vegetable types (N of self-reported portions/day, [99% confidence interval]) by gender and age group (24-h recall)

Age Gender Total fruit (fresh, canned, dried, frozen) Fresh fruit Berry Juice Total vegetables (salad/raw, cooked, canned) Salad/raw vegetables Cooked vegetables
18–24 y Women 1.7 [1.3–2.2] 1.5 [1.1–1.9] 0.2 [0.0–0.3] 1.0 [0.6–1.3] 2.3 [1.7–2.8] 1.5 [1.0–2.0] 0.3 [0.1–0.5]
Men 1.2 [0.8–1.6] 1.1 [0.7–1.5] 0.1 [0.0–0.2] 1.0 [0.6–1.4] 1.8 [1.3–2.3] 1.0 [0.6–1.4] 0.3 [−1–0.6]
25–34 y Women 1.8 [1.4–2.1] 1.6 [1.3–1.9] 0.2 [0.0–0.4] 0.7 [0.4–1.0] 2.3 [1.8–2.9] 1.5 [1.1–1.8] 0.4 [0.2–0.6]
Men 1.2 [0.8–1.5] 1.1 [0.8–1.4] 0.2 [0.1–0.4] 0.9 [0.6–1.2] 2.2 [1.8–2.6] 1.5 [1.1–1.8] 0.4 [0.2–0.6]
35–44 y Women 1.9 [1.5–2.2] 1.8 [1.4–2.1] 0.2 [0.1–0.4] 0.7 [0.4–0.9] 2.0 [1.7–2.4] 1.4 [1.1–1.7] 0.3 [0.2–0.5]
Men 1.7 [1.3–2.0] 1.5 [1.2–1.8] 0.2 [0.1–0.3] 1.0 [0.7–1.3] 1.8 [1.4–2.2] 1.1 [0.7–1.5] 0.3 [0.2–0.5]
45–54 y Women 2.0 [1.6–2.4] 1.8 [1.4–2.1] 0.4 [0.2–0.6] 0.7 [0.5–0.9] 2.3 [1.9–2.8] 1.5 [1.2–1.8] 0.5 [0.3–0.7]
Men 1.7 [1.3–2.0] 1.5 [1.2–1.8] 0.2 [0.1–0.4] 0.6 [0.4–0.8] 2.2 [1.7–2.6] 1.2 [0.9–1.5] 0.6 [0.3–0.8]
55–64 y Women 2.4 [2.0–2.8] 2.2 [1.8–2.6] 0.3 [0.1–0.5] 0.7 [0.5–0.9] 2.4 [2.0–2.7] 1.4 [1.2–1.7] 0.7 [0.4–0.89]
Men 1.7 [1.4–2.0] 1.5 [1.3–1.8] 0.2 [0.1–0.4] 0.7 [0.5–0.9] 1.7 [1.4–2.0] 1.1 [0.9–1.4] 0.4 [0.2–0.6]
P=0.001 P=0.002 P=0.002
65–74 y Women 2.3 [2.0–2.7] 2.1 [1.8–2.4] 0.4 [0.2–0.6] 0.6 [0.4–0.8] 2.2 [1.8–2.5] 1.2 [1.0–1.5] 0.7 [0.4–0.9]
Men 1.7 [1.4–1.9] 1.5 [1.2–1.7] 0.5 [0.3–0.7] 0.7 [0.6–0.9] 2.0 [1.7–2.4] 1.0 [0.8–1.2] 0.6 [0.4–0.8]
P<0.001 P<0.001
75–84 y Women 2.4 [1.9–2.8] 2.2 [1.8–2.6] 0.6 [0.2–1.0] 0.6 [0.3–0.9] 1.9 [1.5–2.4] 1.1 [0.8–1.4] 0.5 [0.3–0.8]
Men 1.9 [1.5–2.2] 1.7 [1.4–2.1] 0.4 [0.3–0.6] 0.6 [0.4–0.8] 1.8 [1.5–2.2] 1.0 [0.7–1.3] 0.6 [0.4–0.8]
All ages Women 2.1 [1.9–2.2] 1.9 [1.7–2.0] 0.3 [0.2–0.4] 0.7 [0.6–0.8] 2.2 [2.0–2.4] 1.4 [1.3–1.5] 0.5 [0.4–0.6]
Men 1.6 [1.4–1.9] 1.5 [1.3–1.6] 0.3 [0.2–0.4] 0.8 [0.7–0.9] 1.9 [1.8–2.1] 1.1 [1.0–1.3] 0.5 [0.4–0.6]
P<0.001 P<0.001 P=0.003 P<0.001

Note: P-values for gender differences within age group. Bolded figures are statistically significant differences.

The FFQ also showed that fresh F&V were consumed most often (Table 3). More women than men in all age groups consumed fresh fruit every day (63.5% vs. 38.9% for all age groups combined, respectively; P<0.001)). Almost one half of women (48.3%) consumed salad or raw vegetables every day compared to 28.8% among men (P<0.001). Men reported a higher consumption frequency only for canned fruit (P=0.002), although the overall consumption frequency was very low.

Table 3.

Percentages of daily usersa of different fruit and vegetable types and gender differences in F&V consumption frequencies within age groups (food frequency questionnaire)

Age Gender Fresh fruit Dried fruit Canned fruit Juice Berries Salad/raw vegetables Cooked vegetables Canned vegetables Beans
18–24 y Women 48.9 6.6 3.3 20.0 5.5 41.3 7.7 6.6 6.5
Men 19.6 5.9 2.0 22.0 2.0 29.4 6.0 0 2.0
P=0.003b
25–34 y Women 56.5 7.3 1.9 19.8 1.8 45.0 13.0 3.7 1.9
Men 35.5 6.6 0 15.8 0 28.4 2.7 1.4 1.4
P<0.001b
35–44 y Women 60.6 7.3 0 18.3 2.7 54.1 12.7 4.7 1.8
Men 38.6 8.6 0 25.7 1.4 34.8 5.8 2.9 1.4
P<0.001b P<0.001b
45–54 y Women 65.5 9.9 0 20.5 9.9 54.5 11.8 1.8 3.7
Men 37.0 0 0 24.1 0 29.6 3.8 2.5 4.9
P<0.001b P=0.001b P=0.007b P<0.001b
55–64 y Women 65.1 14.3 0 23.4 5.5 48.0 12.7 0.8 1.6
Men 43.5 12.0 0 23.3 8.0 33.3 7.1 2.3 3.5
P<0.001b P=0.002b P<0.001b
65–74 y Women 77.7 17.6 0 31.3 14.3 50.9 12.1 1.0 3.0
Men 46.1 12.6 0 22.7 7.1 35.3 5.0 2.0 1.0
P<0.001b P=0.001b
75–84 y Women 71.0 19.6 0 26.9 11.3 38.6 10.7 1.6 1.9
Men 41.6 10.7 1.3 24.1 14.1 30.6 13.8 1.2 2.5
P<0.001b
All ages Women 63.5 11.0 0.3 22.6 6.9 48.3 11.7 1.7 2.9
Men 38.9 8.3 0.4 22.6 5.1 28.8 6.4 1.8 2.4
P<0.001b P<0.001b P=0.001b

Note: Bolded figures are statistically significant differences.

aOnce/day + two times/day + more than twice a day.

bMann–Whitney test for gender differences in consumption frequencies (eight categories as presented in Methods).

Comparisons between women and men using the web-based or pen-and-paper questionnaire showed no differences in total F&V consumption. Age differences between these two groups could not be seen, whereas women choosing the web-based questionnaire had higher level of education.

Discussion

On average, respondents in the present study reported slightly higher F&V consumption than expected based on earlier findings in Sweden (13, 14). Some other recent studies (25) have also reported changes in dietary habits during the past decade. However, the methodology used in the present study differed from the latest national food intake survey (13), and the results are not directly comparable with each others. In line with our findings, previous studies have also shown that men tend to have lower F&V consumption than women (1518). A study from Finland showed that gender differences in vegetable consumption have actually increased (34). Both the self-administered 24-h recall and FFQ showed higher F&V consumption in women when all age groups were combined, while the data from the FFQ showed larger differences between women and men within different age groups. Earlier studies have shown that men slightly underestimate their F&V consumption, whereas the opposite applies to women (33). Thus, the gender differences revealed here may turn out to be slightly less significant, but the similar findings from two measures indicate that these differences actually exist.

The self-administered 24-h recall showed higher consumption of fresh fruit particularly for middle-aged and older women, which is in line with results from a recent Finnish study (18). On the other hand, the FFQ showed that women in many age groups were more frequent consumers of not only fruit, but also salad and other raw vegetables. F&V seem to be most popular when they are fresh. It seems that Swedes are not used to including F&V in mixed dishes, but rather eat them separately as they are. On the other hand, the authors are aware of the limitations of the study questionnaire, where only one open-ended item on mixed dishes was included. This may have led to some underreporting, indicating that actual consumption may be higher than reported.

The present study further showed some interesting differences between women and men in factors associated with F&V consumption. Men born outside Sweden had higher F&V consumption than men born in Sweden did, which may reflect the different habits associated with other food cultures. For instance, non-Western immigrants in Denmark have been reported to have higher odds for daily consumption of cooked and raw vegetables (35), and Iranians living in Sweden also show higher consumption of F&V (36). However, because the specific country of birth is not reported in the present study, no conclusions regarding specific food cultures can be drawn.

PA was the only variable of those investigated that was associated with total F&V consumption in both genders. Therefore, we may speculate that F&V consumption is an integrated part of a lifestyle in which PA also plays an important role. However, the limitations of only using a single question to measure level of PA are recognized (25), and more detailed assessments are encouraged to gain better understanding on the associations between PA and consumption of F&V.

From a methodological point of view, the FFQ may enable better prediction of average consumption over a longer time period, whereas the self-administered 24-h recall was used to more accurately assess consumption over a specified period. Also, there is a certain risk of over-reporting actual consumption in the FFQ, but this was not regarded as a problem when ranking individuals and subgroups according to their consumption (37). In addition, although consumption has not been reported in grams, consumption frequency as such is a good measure of consumption, because portion sizes have not been reported to differ between high and low consumers (12).

We are aware of the possibility of a type I error for false significant findings (38) due to the many significance tests conducted. However, because the main findings are significant at a level well below 1%, and both dietary assessment measures showed similar findings, this is not regarded as a problem. In the present study, all days of the week were equally represented in the data (data not shown), which eliminates the possible bias caused by consumption differences across the weekdays. Seasonal consumption variation can also be excluded as a confounding factor, because the data consist of roughly equally large groups of participants in different seasonal groups. The aim was to show an average consumption of the population over one year and not to compare seasonal variation as such.

A nationwide random sample is not a widely used data collection method. The present results have to be interpreted with caution owing to the relatively large proportion of non-respondents (49%), although the drop-out rate was nearly as high in the latest nationwide dietary survey used as a reference here (13). Men in general, and the youngest age group (18–24 years), but also the oldest women (75–84 years) were somewhat underrepresented in the present survey. However, the relatively high total number of respondents should be representative of actual F&V consumption levels in Sweden, although in most cases, respondent selection in postal surveys probably leads to overrepresentation of people who are more interested in the topic, or who already have healthier dietary habits. This may have resulted in underestimating real differences between different sociodemographic groups.

The cross-sectional design limits conclusions about the temporal or causal nature of the findings, but the study design can provide answers that address the study aims. From a data collection perspective, the present study gives valuable experience in using a web-based questionnaire as an alternative response measure. Giving respondents the opportunity to choose between a traditional pen-and-paper and a web-based questionnaire was intended to increase the response rate and attract particularly younger respondents, but age differences could not be identified in the present study (data not shown). In accordance with Danish experiences (39), the majority of respondents preferred the paper version to the web-based questionnaire. However, using the Internet may still be worthwhile, as it saves both environmental and labor resources.

Conclusions

The present study showed average F&V consumption close to the recommended level of five portions per day. Fresh F&V showed the highest consumption, whereas berries and processed F&V products were less popular. University education was positively associated with higher F&V consumption for women, and men born outside Sweden showed higher consumption than did men born in Sweden. Respondents who reported more hours per week engaged in doing PA had higher F&V consumption in both genders. Although total consumption seems to have increased, the role of other underlying factors besides the sociodemographic factors and PA studied here remains unclear. In addition, other perspectives on F&V consumption, such as possible seasonal variation in availability of different F&V types, should be taken into consideration when implementing new dietary interventions or guidelines.

Acknowledgements

This study was supported by grants from Stiftelsen Uppsala Hushållskolas fond and Fackskolans elevförbund. The authors would like to thank all who took part in this study. All the authors were responsible for fund raising and study design. A.S. performed the data collection, data analysis, and drafted the paper as part of her PhD thesis at Uppsala University. M.N. and A.A. critically revised the manuscript. All the authors approved the final version.

Conflict of interest and funding

The authors have not received any funding or benefits from industry to conduct this study.

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