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. Author manuscript; available in PMC: 2011 Jan 1.
Published in final edited form as: Hippocampus. 2010 Jan;20(1):113–124. doi: 10.1002/hipo.20589

FIGURE 5.

FIGURE 5

Green fluorescent protein (GFP), Fluoro-Jade B (FJB), and cresyl violet (Nissl) staining in a 1 month saline control mouse (left column) and mice 1 month after pilocarpine-induced status epilepticus (SE). The middle column shows sections from an animal with no detectable loss of dentate granule cells, CA3 pyramidal cells or CA1 pyramidal cells (cell loss score = 2 due to hilar damage). The right column shows sections from an animal (cell loss score = 8) with obvious loss of dentate granule cells (arrows) and CA3 pyramidal cells (arrowhead). Loss of GFP labeling, reflecting granule cell death, is also evident in the DG of this animal (asterisk). Fluoro-Jade B staining reveals degenerating fibers in stratum radiatum (SR) and stratum oriens (SO) of CA1 in this animal. Staining on the lower edge of the dentate and upper edge of the adjacent thalamus is artifactual (“edge artifact”). Scale bar = 500 μm. [Color figure can be viewed in the online issue, which is available at www.interscience.wiley.com.]