Skip to main content
NCBI home page
Proceedings of the National Academy of Sciences of the United States of America logoLink to Proceedings of the National Academy of Sciences of the United States of America
. 1988 May;85(10):3358–3362. doi: 10.1073/pnas.85.10.3358

Carboxyl-terminal amino acid sequences of two variant forms of the gamma chain of human plasma fibrinogen.

C W Francis 1, E Müller 1, A Henschen 1, P J Simpson 1, V J Marder 1
PMCID: PMC280208  PMID: 3368448

Abstract

The gamma chain of human fibrinogen has been shown to be heterogeneous; three forms of various sizes are normally present in plasma. To further characterize the two less prevalent elongated variants, we have purified the three forms of the gamma chain, isolated unique tryptic fragments, and sequenced the variant portions of each chain. The intermediate-sized form (gamma 55) has a sequence identical to the longest variant (gamma 57.5) from residue Val-408 to Pro-423, which is the C terminus of the gamma 55 chain. The gamma 57.5 chain extends an additional four amino acids. The C-terminal amino acid sequence and corresponding nucleotide sequence of the gamma 55 chain show marked similarity with an elongated gamma-chain variant of rat fibrinogen. In addition, a remarkable similarity in both amino acid and nucleotide sequence was noted between the human gamma 55 chain and the C-terminal extension in human and bovine A alpha chain of fibrinogen, which are encoded by the cDNA but are posttranslationally cleaved and are not found in plasma. The findings suggest an evolutionary conservation in the C-terminal amino acid sequence found in both the fibrinogen A alpha and gamma chains in several species.

Full text

PDF
3358

Images in this article

3359Image
on p.3359

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Chung D. W., Chan W. Y., Davie E. W. Characterization of a complementary deoxyribonucleic acid coding for the gamma chain of human fibrinogen. Biochemistry. 1983 Jun 21;22(13):3250–3256. doi: 10.1021/bi00282a033. [DOI] [PubMed] [Google Scholar]
  2. Chung D. W., Davie E. W. gamma and gamma' chains of human fibrinogen are produced by alternative mRNA processing. Biochemistry. 1984 Aug 28;23(18):4232–4236. doi: 10.1021/bi00313a033. [DOI] [PubMed] [Google Scholar]
  3. Crabtree G. R., Comeau C. M., Fowlkes D. M., Fornace A. J., Jr, Malley J. D., Kant J. A. Evolution and structure of the fibrinogen genes. Random insertion of introns or selective loss? J Mol Biol. 1985 Sep 5;185(1):1–19. doi: 10.1016/0022-2836(85)90179-2. [DOI] [PubMed] [Google Scholar]
  4. Crabtree G. R., Kant J. A., Fornace A. J., Jr, Rauch C. A., Fowlkes D. M. Regulation and characterization of the mRNAs for the A alpha, B beta and gamma chains of fibrinogen. Ann N Y Acad Sci. 1983 Jun 27;408:457–468. doi: 10.1111/j.1749-6632.1983.tb23266.x. [DOI] [PubMed] [Google Scholar]
  5. Crabtree G. R., Kant J. A. Organization of the rat gamma-fibrinogen gene: alternative mRNA splice patterns produce the gamma A and gamma B (gamma ') chains of fibrinogen. Cell. 1982 Nov;31(1):159–166. doi: 10.1016/0092-8674(82)90415-9. [DOI] [PubMed] [Google Scholar]
  6. Deutsch D. G., Mertz E. T. Plasminogen: purification from human plasma by affinity chromatography. Science. 1970 Dec 4;170(3962):1095–1096. doi: 10.1126/science.170.3962.1095. [DOI] [PubMed] [Google Scholar]
  7. Doolittle R. F., Watt K. W., Cottrell B. A., Strong D. D., Riley M. The amino acid sequence of the alpha-chain of human fibrinogen. Nature. 1979 Aug 9;280(5722):464–468. doi: 10.1038/280464a0. [DOI] [PubMed] [Google Scholar]
  8. Engvall E., Ruoslahti E. Binding of soluble form of fibroblast surface protein, fibronectin, to collagen. Int J Cancer. 1977 Jul 15;20(1):1–5. doi: 10.1002/ijc.2910200102. [DOI] [PubMed] [Google Scholar]
  9. FINLAYSON J. S., MOSESSON M. W. CHROMATOGRAPHIC HETEROGENEITY OF ANIMAL FIBRINOGENS. Biochim Biophys Acta. 1964 Feb 10;82:415–417. doi: 10.1016/0304-4165(64)90318-6. [DOI] [PubMed] [Google Scholar]
  10. Fornace A. J., Jr, Cummings D. E., Comeau C. M., Kant J. A., Crabtree G. R. Structure of the human gamma-fibrinogen gene. Alternate mRNA splicing near the 3' end of the gene produces gamma A and gamma B forms of gamma-fibrinogen. J Biol Chem. 1984 Oct 25;259(20):12826–12830. [PubMed] [Google Scholar]
  11. Francis C. W., Keele E. M., Marder V. J. Purification of three gamma-chains with different molecular weights from normal human plasma fibrinogen. Biochim Biophys Acta. 1984 Mar 1;797(3):328–335. doi: 10.1016/0304-4165(84)90253-8. [DOI] [PubMed] [Google Scholar]
  12. Francis C. W., Kraus D. H., Marder V. J. Structural and chromatographic heterogeneity of normal plasma fibrinogen associated with the presence of three gamma-chain types with distinct molecular weights. Biochim Biophys Acta. 1983 Apr 28;744(2):155–164. doi: 10.1016/0167-4838(83)90085-7. [DOI] [PubMed] [Google Scholar]
  13. Francis C. W., Marder V. J., Martin S. E. Demonstration of a large molecular weight variant of the gamma chain of normal human plasma fibrinogen. J Biol Chem. 1980 Jun 25;255(12):5599–5604. [PubMed] [Google Scholar]
  14. Henschen A., Lottspeich F. Sequence homology between gamma-chain and beta-chain in human fibrin. Thromb Res. 1977 Dec;11(6):869–880. doi: 10.1016/0049-3848(77)90115-3. [DOI] [PubMed] [Google Scholar]
  15. Homandberg G. A., Williams J. E., Evans D. B., Mosesson M. W. Evidence that rat platelet fibrinogen molecules lack the gamma' chain variant found in plasma fibrinogen molecules. Thromb Res. 1985 Jul 15;39(2):203–209. doi: 10.1016/0049-3848(85)90108-2. [DOI] [PubMed] [Google Scholar]
  16. Kant J. A., Fornace A. J., Jr, Saxe D., Simon M. I., McBride O. W., Crabtree G. R. Evolution and organization of the fibrinogen locus on chromosome 4: gene duplication accompanied by transposition and inversion. Proc Natl Acad Sci U S A. 1985 Apr;82(8):2344–2348. doi: 10.1073/pnas.82.8.2344. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Kant J. A., Lord S. T., Crabtree G. R. Partial mRNA sequences for human A alpha, B beta, and gamma fibrinogen chains: evolutionary and functional implications. Proc Natl Acad Sci U S A. 1983 Jul;80(13):3953–3957. doi: 10.1073/pnas.80.13.3953. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Morgan J. G., Holbrook N. J., Crabtree G. R. Nucleotide sequence of the gamma chain gene of rat fibrinogen: conserved intronic sequences. Nucleic Acids Res. 1987 Mar 25;15(6):2774–2776. doi: 10.1093/nar/15.6.2774. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Murano G., Wiman B., Blombäck M., Blombäck B. Preparation and isolation of the S-carboxymethyl derivative chains of human fibrinogen. FEBS Lett. 1971 Apr 12;14(1):37–41. doi: 10.1016/0014-5793(71)80269-7. [DOI] [PubMed] [Google Scholar]
  20. Rixon M. W., Chan W. Y., Davie E. W., Chung D. W. Characterization of a complementary deoxyribonucleic acid coding for the alpha chain of human fibrinogen. Biochemistry. 1983 Jun 21;22(13):3237–3244. doi: 10.1021/bi00282a031. [DOI] [PubMed] [Google Scholar]
  21. Rixon M. W., Chung D. W., Davie E. W. Nucleotide sequence of the gene for the gamma chain of human fibrinogen. Biochemistry. 1985 Apr 9;24(8):2077–2086. doi: 10.1021/bi00329a041. [DOI] [PubMed] [Google Scholar]
  22. Stathakis N. E., Mosesson M. W., Galanakis D. K., Ménaché D. Human fibrinogen heterogeneities. Preparation and characterization of gamma and gamma' chains. Thromb Res. 1978 Sep;13(3):467–475. doi: 10.1016/0049-3848(78)90132-9. [DOI] [PubMed] [Google Scholar]
  23. Weber K., Osborn M. The reliability of molecular weight determinations by dodecyl sulfate-polyacrylamide gel electrophoresis. J Biol Chem. 1969 Aug 25;244(16):4406–4412. [PubMed] [Google Scholar]
  24. Wolfenstein-Todel C., Mosesson M. W. Carboxy-terminal amino acid sequence of a human fibrinogen gamma-chain variant (gamma'). Biochemistry. 1981 Oct 13;20(21):6146–6149. doi: 10.1021/bi00524a036. [DOI] [PubMed] [Google Scholar]
  25. Wolfenstein-Todel C., Mosesson M. W. Human plasma fibrinogen heterogeneity: evidence for an extended carboxyl-terminal sequence in a normal gamma chain variant (gamma'). Proc Natl Acad Sci U S A. 1980 Sep;77(9):5069–5073. doi: 10.1073/pnas.77.9.5069. [DOI] [PMC free article] [PubMed] [Google Scholar]
  26. Yoshimoto T., Walter R., Tsuru D. Proline-specific endopeptidase from Flavobacterium. Purification and properties. J Biol Chem. 1980 May 25;255(10):4786–4792. [PubMed] [Google Scholar]

Articles from Proceedings of the National Academy of Sciences of the United States of America are provided here courtesy of National Academy of Sciences

RESOURCES