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Proceedings of the National Academy of Sciences of the United States of America logoLink to Proceedings of the National Academy of Sciences of the United States of America
. 1988 May;85(10):3417–3421. doi: 10.1073/pnas.85.10.3417

Hairy roots are more sensitive to auxin than normal roots

Wen Hui Shen *,†,, Annik Petit , Jean Guern *, Jacques Tempé
PMCID: PMC280222  PMID: 16593928

Abstract

Responses to auxin of Lotus corniculatus root tips or protoplasts transformed by Agrobacterium rhizogenes strains 15834 and 8196 were compared to those of their normal counterparts. Three different types of experiments were performed, involving long-term, medium-term, or short-term responses to a synthetic auxin, 1-naphthaleneacetic acid. Root tip elongation, proton excretion by root tips, and transmembrane electrical potential difference of root protoplasts were measured as a function of exogenous auxin concentration. The sensitivity of hairy root tips or protoplasts to exogenous auxin was found to be 100-1000 times higher than that of untransformed material.

Keywords: Agrobacterium rhizogenes, root elongation, proton excretion, transmembrane potential, 1-naphthaleneacetic acid

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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