Abstract
To understand the molecular basis of the phenotype of gypsy-induced mutations, we have analyzed the structure of phenotypic revertants of the y2 allele, which is caused by the insertion of the gypsy element into the 5' region of the yellow (y) locus. Seven spontaneous revertants examined fall into two different classes. Three of these revertants arose by homologous recombination between the two gypsy long terminal repeats (LTRs), leaving behind a solo LTR. Four additional revertants contain an intact 3' LTR and half of the 5' LTR, but the central portion of gypsy has been replaced by a different 6.5-kilobase transposable element that contains a poly(A) tail. These results suggest that the mutagenic effect of the gypsy element is not due to its insertion into sequences necessary for transcription or to the distancing between the yellow promoter and remote regulatory sequences but is a consequence of idiosyncratic properties of the element itself.
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Selected References
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- Biessmann H. Molecular analysis of the yellow gene (y) region of Drosophila melanogaster. Proc Natl Acad Sci U S A. 1985 Nov;82(21):7369–7373. doi: 10.1073/pnas.82.21.7369. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Campuzano S., Carramolino L., Cabrera C. V., Ruíz-Gómez M., Villares R., Boronat A., Modolell J. Molecular genetics of the achaete-scute gene complex of D. melanogaster. Cell. 1985 Feb;40(2):327–338. doi: 10.1016/0092-8674(85)90147-3. [DOI] [PubMed] [Google Scholar]
- Chia W., Howes G., Martin M., Meng Y. B., Moses K., Tsubota S. Molecular analysis of the yellow locus of Drosophila. EMBO J. 1986 Dec 20;5(13):3597–3605. doi: 10.1002/j.1460-2075.1986.tb04688.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Di Nocera P. P., Casari G. Related polypeptides are encoded by Drosophila F elements, I factors, and mammalian L1 sequences. Proc Natl Acad Sci U S A. 1987 Aug;84(16):5843–5847. doi: 10.1073/pnas.84.16.5843. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Di Nocera P. P., Digan M. E., Dawid I. B. A family of oligo-adenylate-terminated transposable sequences in Drosophila melanogaster. J Mol Biol. 1983 Aug 25;168(4):715–727. doi: 10.1016/s0022-2836(83)80071-0. [DOI] [PubMed] [Google Scholar]
- Freund R., Meselson M. Long terminal repeat nucleotide sequence and specific insertion of the gypsy transposon. Proc Natl Acad Sci U S A. 1984 Jul;81(14):4462–4464. doi: 10.1073/pnas.81.14.4462. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Geyer P. K., Corces V. G. Separate regulatory elements are responsible for the complex pattern of tissue-specific and developmental transcription of the yellow locus in Drosophila melanogaster. Genes Dev. 1987 Nov;1(9):996–1004. doi: 10.1101/gad.1.9.996. [DOI] [PubMed] [Google Scholar]
- Geyer P. K., Spana C., Corces V. G. On the molecular mechanism of gypsy-induced mutations at the yellow locus of Drosophila melanogaster. EMBO J. 1986 Oct;5(10):2657–2662. doi: 10.1002/j.1460-2075.1986.tb04548.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Green M. M., Lefevre G., Jr The cytogenetics of mutator gene-induced X-linked lethals in Drosophila melanogaster. Mutat Res. 1972 Sep;16(7):59–64. doi: 10.1016/0027-5107(72)90064-4. [DOI] [PubMed] [Google Scholar]
- Green M. M. The genetics of a mutator gene in Drosophila melanogaster. Mutat Res. 1970 Oct;10(4):353–363. doi: 10.1016/0027-5107(70)90048-5. [DOI] [PubMed] [Google Scholar]
- Marlor R. L., Parkhurst S. M., Corces V. G. The Drosophila melanogaster gypsy transposable element encodes putative gene products homologous to retroviral proteins. Mol Cell Biol. 1986 Apr;6(4):1129–1134. doi: 10.1128/mcb.6.4.1129. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Maxam A. M., Gilbert W. Sequencing end-labeled DNA with base-specific chemical cleavages. Methods Enzymol. 1980;65(1):499–560. doi: 10.1016/s0076-6879(80)65059-9. [DOI] [PubMed] [Google Scholar]
- Mizrokhi L. J., Obolenkova L. A., Priimägi A. F., Ilyin Y. V., Gerasimova T. I., Georgiev G. P. The nature of unstable insertion mutations and reversions in the locus cut of Drosophila melanogaster: molecular mechanism of transposition memory. EMBO J. 1985 Dec 30;4(13B):3781–3787. doi: 10.1002/j.1460-2075.1985.tb04148.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Modolell J., Bender W., Meselson M. Drosophila melanogaster mutations suppressible by the suppressor of Hairy-wing are insertions of a 7.3-kilobase mobile element. Proc Natl Acad Sci U S A. 1983 Mar;80(6):1678–1682. doi: 10.1073/pnas.80.6.1678. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Mount S. M., Green M. M., Rubin G. M. Partial revertants of the transposable element-associated suppressible allele white-apricot in Drosophila melanogaster: structures and responsiveness to genetic modifiers. Genetics. 1988 Feb;118(2):221–234. doi: 10.1093/genetics/118.2.221. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Mozer B., Marlor R., Parkhurst S., Corces V. Characterization and developmental expression of a Drosophila ras oncogene. Mol Cell Biol. 1985 Apr;5(4):885–889. doi: 10.1128/mcb.5.4.885. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Parkhurst S. M., Corces V. G. Forked, gypsys, and suppressors in Drosophila. Cell. 1985 Jun;41(2):429–437. doi: 10.1016/s0092-8674(85)80016-7. [DOI] [PubMed] [Google Scholar]
- Parkhurst S. M., Corces V. G. Interactions among the gypsy transposable element and the yellow and the suppressor of hairy-wing loci in Drosophila melanogaster. Mol Cell Biol. 1986 Jan;6(1):47–53. doi: 10.1128/mcb.6.1.47. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Parkhurst S. M., Corces V. G. Retroviral elements and suppressor genes in Drosophila. Bioessays. 1986 Aug;5(2):52–57. doi: 10.1002/bies.950050203. [DOI] [PubMed] [Google Scholar]
- Spradling A. C., Mahowald A. P. Identification and genetic localization of mRNAs from ovarian follicle cells of Drosophila melanogaster. Cell. 1979 Mar;16(3):589–598. doi: 10.1016/0092-8674(79)90032-1. [DOI] [PubMed] [Google Scholar]