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. 1988 Jun;85(11):4025–4029. doi: 10.1073/pnas.85.11.4025

Specific and stable labeling of antibodies with technetium-99m with a diamide dithiolate chelating agent.

A R Fritzberg 1, P G Abrams 1, P L Beaumier 1, S Kasina 1, A C Morgan 1, T N Rao 1, J M Reno 1, J A Sanderson 1, A Srinivasan 1, D S Wilbur 1, et al.
PMCID: PMC280353  PMID: 3375252

Abstract

Technetium-99m labeling of antibodies has been suboptimal because of low affinity adventitious binding, nonspecific labeling, and loss of immunoreactivity. The diamide dithiolate ligand system (N2S2) forms highly stable, well-defined tetradentate complexes with Tc(V). Antibodies and their fragments have been labeled by conjugation of preformed 99mTc-4,5-bis(thioacetamido)pentanoate active ester to protein amine groups to give a chemically known 99mTc-N2S2 complex covalently linked to antibody. Evaluations of the 99mTc-N2S2-bound antibodies and their fragments have shown high stability and retained immunoreactivity.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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