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. 1988 Jul;85(13):4809–4813. doi: 10.1073/pnas.85.13.4809

An intermediate in transposition of the conjugative transposon Tn916.

J R Scott 1, P A Kirchman 1, M G Caparon 1
PMCID: PMC280525  PMID: 2838847

Abstract

Using the conjugative transposon Tn916, we have identified a closed covalent circular form produced in vivo that is able to serve as an intermediate in transposition. When a region of a streptococcal chromosome containing Tn916 is cloned in an Escherichia coli plasmid, supercoiled transposon molecules are excised spontaneously. The purified supercoiled forms transform Bacillus subtilis protoplasts by inserting into the chromosome, apparently at random. In B. subtilis, Tn916 retains its ability to promote conjugative transposition, as shown by its transfer to Streptococcus pyogenes.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Caillaud F., Carlier C., Courvalin P. Physical analysis of the conjugative shuttle transposon Tn1545. Plasmid. 1987 Jan;17(1):58–60. doi: 10.1016/0147-619x(87)90009-6. [DOI] [PubMed] [Google Scholar]
  2. Caillaud F., Courvalin P. Nucleotide sequence of the ends of the conjugative shuttle transposon Tn1545. Mol Gen Genet. 1987 Aug;209(1):110–115. doi: 10.1007/BF00329844. [DOI] [PubMed] [Google Scholar]
  3. Capage M., Hill C. W. Preferential unequal recombination in the glyS region of the Escherichia coli chromosome. J Mol Biol. 1979 Jan 5;127(1):73–87. doi: 10.1016/0022-2836(79)90460-1. [DOI] [PubMed] [Google Scholar]
  4. Caparon M. G., Scott J. R. Identification of a gene that regulates expression of M protein, the major virulence determinant of group A streptococci. Proc Natl Acad Sci U S A. 1987 Dec;84(23):8677–8681. doi: 10.1073/pnas.84.23.8677. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Chang S., Cohen S. N. High frequency transformation of Bacillus subtilis protoplasts by plasmid DNA. Mol Gen Genet. 1979 Jan 5;168(1):111–115. doi: 10.1007/BF00267940. [DOI] [PubMed] [Google Scholar]
  6. Christie P. J., Korman R. Z., Zahler S. A., Adsit J. C., Dunny G. M. Two conjugation systems associated with Streptococcus faecalis plasmid pCF10: identification of a conjugative transposon that transfers between S. faecalis and Bacillus subtilis. J Bacteriol. 1987 Jun;169(6):2529–2536. doi: 10.1128/jb.169.6.2529-2536.1987. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Clewell D. B., Gawron-Burke C. Conjugative transposons and the dissemination of antibiotic resistance in streptococci. Annu Rev Microbiol. 1986;40:635–659. doi: 10.1146/annurev.mi.40.100186.003223. [DOI] [PubMed] [Google Scholar]
  8. Courvalin P., Carlier C. Tn1545: a conjugative shuttle transposon. Mol Gen Genet. 1987 Feb;206(2):259–264. doi: 10.1007/BF00333582. [DOI] [PubMed] [Google Scholar]
  9. Courvalin P., Carlier C. Transposable multiple antibiotic resistance in Streptococcus pneumoniae. Mol Gen Genet. 1986 Nov;205(2):291–297. doi: 10.1007/BF00430441. [DOI] [PubMed] [Google Scholar]
  10. Derbyshire K. M., Grindley N. D. Replicative and conservative transposition in bacteria. Cell. 1986 Nov 7;47(3):325–327. doi: 10.1016/0092-8674(86)90586-6. [DOI] [PubMed] [Google Scholar]
  11. Feinberg A. P., Vogelstein B. A technique for radiolabeling DNA restriction endonuclease fragments to high specific activity. Anal Biochem. 1983 Jul 1;132(1):6–13. doi: 10.1016/0003-2697(83)90418-9. [DOI] [PubMed] [Google Scholar]
  12. Fischetti V. A., Jones K. F., Scott J. R. Size variation of the M protein in group A streptococci. J Exp Med. 1985 Jun 1;161(6):1384–1401. doi: 10.1084/jem.161.6.1384. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Fitzgerald G. F., Clewell D. B. A conjugative transposon (Tn919) in Streptococcus sanguis. Infect Immun. 1985 Feb;47(2):415–420. doi: 10.1128/iai.47.2.415-420.1985. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Franke A. E., Clewell D. B. Evidence for a chromosome-borne resistance transposon (Tn916) in Streptococcus faecalis that is capable of "conjugal" transfer in the absence of a conjugative plasmid. J Bacteriol. 1981 Jan;145(1):494–502. doi: 10.1128/jb.145.1.494-502.1981. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Gawron-Burke C., Clewell D. B. A transposon in Streptococcus faecalis with fertility properties. Nature. 1982 Nov 18;300(5889):281–284. doi: 10.1038/300281a0. [DOI] [PubMed] [Google Scholar]
  16. Gawron-Burke C., Clewell D. B. Regeneration of insertionally inactivated streptococcal DNA fragments after excision of transposon Tn916 in Escherichia coli: strategy for targeting and cloning of genes from gram-positive bacteria. J Bacteriol. 1984 Jul;159(1):214–221. doi: 10.1128/jb.159.1.214-221.1984. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Grindley N. D., Reed R. R. Transpositional recombination in prokaryotes. Annu Rev Biochem. 1985;54:863–896. doi: 10.1146/annurev.bi.54.070185.004243. [DOI] [PubMed] [Google Scholar]
  18. Hanahan D. Studies on transformation of Escherichia coli with plasmids. J Mol Biol. 1983 Jun 5;166(4):557–580. doi: 10.1016/s0022-2836(83)80284-8. [DOI] [PubMed] [Google Scholar]
  19. Horodniceanu T., Bougueleret L., Bieth G. Conjugative transfer of multiple-antibiotic resistance markers in beta-hemolytic group A, B, F, and G streptococci in the absence of extrachromosomal deoxyribonucleic acid. Plasmid. 1981 Mar;5(2):127–137. doi: 10.1016/0147-619x(81)90014-7. [DOI] [PubMed] [Google Scholar]
  20. Ionesco H., Michel J., Cami B., Schaeffer P. Symposium on bacterial spores: II. Genetics of sporulation in Bacillus subtilis Marburg. J Appl Bacteriol. 1970 Mar;33(1):13–24. doi: 10.1111/j.1365-2672.1970.tb05230.x. [DOI] [PubMed] [Google Scholar]
  21. Landman O. E., Ryter A., Fréhel C. Gelatin-induced reversion of protoplasts of Bacillus subtilis to the bacillary form: electron-microscopic and physical study. J Bacteriol. 1968 Dec;96(6):2154–2170. doi: 10.1128/jb.96.6.2154-2170.1968. [DOI] [PMC free article] [PubMed] [Google Scholar]
  22. Meinkoth J., Wahl G. Hybridization of nucleic acids immobilized on solid supports. Anal Biochem. 1984 May 1;138(2):267–284. doi: 10.1016/0003-2697(84)90808-x. [DOI] [PubMed] [Google Scholar]
  23. Mottes M., Grandi G., Sgaramella V., Canosi U., Morelli G., Trautner T. A. Different specific activities of the monomeric and oligomeric forms of plasmid DNA in transformation of B. subtilis and E. coli. Mol Gen Genet. 1979 Jul 24;174(3):281–286. doi: 10.1007/BF00267800. [DOI] [PubMed] [Google Scholar]
  24. Ray C., Hay R. E., Carter H. L., Moran C. P., Jr Mutations that affect utilization of a promoter in stationary-phase Bacillus subtilis. J Bacteriol. 1985 Aug;163(2):610–614. doi: 10.1128/jb.163.2.610-614.1985. [DOI] [PMC free article] [PubMed] [Google Scholar]
  25. Scott J. R. A turbid plaque-forming mutant of phage P1 that cannot lysogenize Escherichia coli. Virology. 1974 Dec;62(2):344–349. doi: 10.1016/0042-6822(74)90397-3. [DOI] [PubMed] [Google Scholar]
  26. Scott J. R., Fischetti V. A. Expression of streptococcal M protein in Escherichia coli. Science. 1983 Aug 19;221(4612):758–760. doi: 10.1126/science.6192499. [DOI] [PubMed] [Google Scholar]
  27. Scott J. R., Guenthner P. C., Malone L. M., Fischetti V. A. Conversion of an M- group A streptococcus to M+ by transfer of a plasmid containing an M6 gene. J Exp Med. 1986 Nov 1;164(5):1641–1651. doi: 10.1084/jem.164.5.1641. [DOI] [PMC free article] [PubMed] [Google Scholar]
  28. Wirth R., An F. Y., Clewell D. B. Highly efficient protoplast transformation system for Streptococcus faecalis and a new Escherichia coli-S. faecalis shuttle vector. J Bacteriol. 1986 Mar;165(3):831–836. doi: 10.1128/jb.165.3.831-836.1986. [DOI] [PMC free article] [PubMed] [Google Scholar]

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