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. 1988 Jul;85(13):4857–4860. doi: 10.1073/pnas.85.13.4857

Regulation of protein kinase C by extracellular calcium in bovine parathyroid cells.

N Kobayashi 1, J Russell 1, D Lettieri 1, L M Sherwood 1
PMCID: PMC280535  PMID: 3387442

Abstract

Regulation of protein kinase C in the parathyroid gland was investigated by testing the effects of phorbol ester, exogenous phospholipase C, and low and high calcium concentrations on enzyme activity. Treatment of bovine parathyroid cells with phorbol ester, which activates protein kinase C directly, and with phospholipase C, which produces diacylglycerol, an activator of protein kinase C, significantly stimulated protein kinase C activity. Both agents also enhanced the release of parathyroid hormone. Acute exposure of bovine parathyroid cells to low extracellular calcium (0.5 mM) caused a 5- to 6-fold increase in protein kinase C activity associated with the particulate fraction. In contrast, high extracellular calcium (1.75 mM and 2.5 mM) markedly decreased membrane protein kinase C activity. These data suggest that the effects of extracellular calcium on parathyroid hormone secretion are due, at least in part, to regulation of protein kinase C activity in the parathyroid-cell membrane.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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