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. Author manuscript; available in PMC: 2010 Dec 22.
Published in final edited form as: Biochemistry. 2009 Dec 22;48(50):11902–11911. doi: 10.1021/bi901600f

Figure 2.

Figure 2

Effects of COX inhibitors on EPR spectra of preformed tyrosyl radicals in PGHS-2. The holoenzyme (11 μM) was reacted at 0 °C with 2 eq of EtOOH for 6-8 s before addition of 20 eq of inhibitor and 6-7 s of further reaction before freezing and recording the initial EPR spectrum (0s). The control sample was frozen after reaction with peroxide, without addition of inhibitor. The subsequent spectra were recorded after warming the samples in an ice bath for the indicated cumulative lengths of time and refreezing. The arrows indicate the direction of amplitude change with time.