Species concepts for trypanosomes: from morphological to molecular definitions?

Abstract

The way species and subspecies names are applied in African trypanosomes of subgenera Trypanozoon and Nannomonas is reviewed in the light of data from molecular taxonomy. In subgenus Trypanozoon the taxonomic importance of pathogenicity, host range and distribution appear to have been inflated relative to actual levels of genetic divergence. The opposite is true for subgenus Nannomonas, where current taxonomic usage badly underrepresents genetic diversity. Data from molecular characterisation studies are revealing a growing number of genotypes, which may represent distinct taxa. Unfortunately few of these genotypes are yet supported by sufficient biological data to be recognized taxonomically. But we may be missing fundamental epidemiological information, because of our inability to distinguish these trypanosomes in host blood morphologically or in tsetse by their developmental cycle. Molecular taxonomy has led the way in identifying these new genotypes and now offers the key to elucidating the biology of these organisms.

The concept of a species

In our daily lives we are surrounded by animals and plants that belong to distinct and quite clearly demarcated species. Even little children know that dogs and cats, or apples and pears, are different, and so we come to expect that different species should look different. Yet there is no biological imperative dictating this. To paraphrase an eminent entomologist, the stripes on the legs of mosquitoes are not there for the taxonomist's benefit to facilitate the identification of different species. On the other hand, there may be striking phenotypic variations between individuals of the same species, as exemplified by dogs and other domestic species, that are considered to have no taxonomic relevance.

Taxonomy is traditionally based on morphological differences, but identification of species by morphology is not without pitfalls. How do taxonomists decide what level of morphological difference defines a species? Biologists believe in the concept that (eukaryote) species are defined by the ability of individuals to mate and produce viable and fertile offspring. In practice, this is never actually put to the test in the majority of cases. Instead, a taxonomist with expert knowledge of the group of organisms, extrapolates from detailed information on a few species to make judgements on the group as a whole. The key taxonomic characters defining species will vary from group to group. Unfortunately, the biological species concept offers no guidance on the taxonomy of asexually reproducing organisms.

How does taxonomy based on molecular characters fit into this conceptual framework? Underlying molecular taxonomy is the idea that non-interbreeding populations will diverge genetically. Therefore genetically similar individuals belong to the same species. The difficulty arises in determining what level of similarity defines a species, and just how long ago the event took place that separated 2 lineages. Just as with morphological characters, it takes expert knowledge of the extent of variation within and between known species in the group of organisms to make a judgement on what level of difference constitutes a new species. Again the key taxonomic characters (genes) used for each group of organisms may differ, and even when the same gene is used, for example the 18S ribosomal RNA gene, different levels of variation may prove significant in defining species.

So much for theory – how does this work in practice? It is illuminating to compare taxonomic ideas for two subgenera of African tsetse-transmitted trypanosomes. In subgenus Trypanozoon, taxonomy has been based largely on pathogenicity, distribution and host range. How do these criteria compare to observed levels of genetic divergence? For subgenus Nannomonas, data from molecular characterisation studies are revealing a growing number of distinct genotypes. Does each of these genotypes represent a distinct biological entity and does it matter?

Species in subgenus Trypanozoon

It is generally accepted that subgenus Trypanozoon is divided into 3 species: Trypanosoma brucei, T. evansi and T. equiperdum, with T. brucei further subdivided into 3 subspecies defined by pathogenicity, distribution and host range [1]. Bloodstream form trypanosomes of the 3 species are morphologically indistinguishable, save for the occurrence of short-stumpy forms in T. brucei. Confusingly, the trait of pleomorphism can be lost in laboratory isolates of T. brucei, and they then become indistinguishable from the monomorphic species, T. evansi and T. equiperdum.

At the functional level pleomorphism reflects the ability of T. brucei to develop in its vector, the tsetse fly, and this is in turn dependent on possession of a complete and functional set of genes for mitochondrial operation. The mitochondrial genome is contained in the maxicircle DNA of the kinetoplast of T. brucei, together with the set of minicircle-encoded genes necessary for editing the maxicircle transcripts so they can be correctly translated. These features define T. brucei, and their absence defines T. evansi and T. equiperdum (Table 1). Neither T. evansi or T. equiperdum is cyclically transmitted by tsetse (although tsetse potentially could transmit T. evansi mechanically), and indeed, neither species is capable of cyclical development. T. evansi lacks a mitochondrial genome and its kinetoplast contains only a homogeneous set of minicircles. The few isolates of T. equiperdum examined also have missing kinetoplast DNA. One Chinese strain of T. equiperdum had maxicircles just over half the size of those of T. brucei and homogeneous minicircles like T. evansi [2]. Two other laboratory strains of T. equiperdum also had homogeneous minicircles; one had full-size and one reduced size maxicircles [3,4]. Examination of nuclear DNA polymorphisms by isoenzymes, RFLP, karyotype, minisatellite or phylogenetic analysis has shown no obvious differences between T. evansi, T. equiperdum and T. brucei [2,5-8]

Table 1.

Characteristics of species within subgenus Trypanozoon See text for explanation.

Species	Distribution	Host range	Transmission	Pleomorphic	Differentiation to procyclics	Kinetoplast DNA
						Maxicircles	Heterogeneous minicircles	Homogeneous minicircles
Trypanosoma brucei	Tropical Africa	Wild & domestic mammals	Tsetse – cyclical transmission	+	+	+	+	-
Trypanosoma evansi	North Africa, Asia, S. America	Camels, horses, dogs, bovids	Bloodsucking flies – mechanical transmission	-	-	-	-	+
Trypanosoma equiperdum	Cosmopolitan	Horses	Transmitted via copulation	-	-	+/-	-	+

In a sense then, T. evansi and T. equiperdum can both be regarded as natural mutants of T. brucei. Do they deserve separate species status? Arguably yes, because both satisfy the biological species definition above of non-interbreeding populations. Since genetic exchange in T. brucei takes place during cyclical development in the tsetse fly [9], this excludes participation of either T. evansi or T. equiperdum.

Subspecies of Trypanosoma brucei

Trypanosoma brucei consists of 3 morphologically indistinguishable subspecies, all of which started as full species [1]. The demotion to subspecies came about after recognition that the biological differences between the 3 species were not that significant and could mostly be explained by host range variation and geographical distribution (Table 2). Molecular evidence has not changed that view for T. b. brucei and T. b. rhodesiense. These 2 subspecies share the same range of genetic polymorphisms [8,10-13] and have been demonstrated to interbreed in the laboratory [14]. Most compellingly, it has now been demonstrated that T. b. brucei and T. b. rhodesiense can differ by as little as the expression of a single gene [15-17]. In fact there is a greater level of genetic variation between different T. b. brucei isolates than between T. b. brucei and T. b. rhodesiense [13,18,19].

Table 2.

Characteristics of subspecies and subgroups within Trypanosoma brucei See text for explanation

Subspecies	Distribution	Host range	Transmission	Growth in rodents	Genetic variability	Presence of SRA gene
Trypanosoma brucei brucei	Tropical Africa	Wild & domestic mammals, not humans	Tsetse	Fast	+++	-
Trypanosoma brucei rhodesiense	East Africa	Humans, wild & domestic mammals	Morsitans group tsetse	Fast	++	+
Trypanosoma brucei gambiense Group 1	West & Central Africa	Humans, wild & domestic mammals	Palpalis group tsetse	Slow	+	-
Trypanosoma brucei gambiense Group 2	Ivory Coast	Humans, wild & domestic mammals	Palpalis or morsitans group tsetse?	Fast	++	-

The majority of T. b. gambiense isolates form a homogeneous group (group 1) that stands apart from the rest of the T. brucei group, because of its restricted range of genetic polymorphisms and limited antigenic repertoire [20-24]. It is clear that there is a much greater genetic distance between T. b. gambiense group 1 and other T. brucei subspecies than between T. b. brucei and T. b. rhodesiense. T. b. gambiense group 1 conforms to the classical concept of T. b. gambiense as a slow growing parasite in experimental rodents in contrast to the typically fast growing T. b. brucei/T. b. rhodesiense phenotype. If T. b. gambiense group 1 is genetically isolated, there may be a case for reinstating it as the species T. gambiense. But several questions need to be answered first.

Importantly, can T. b. gambiense group 1 undergo genetic exchange with T. b. brucei? These experiments are not easy, because T. b. gambiense group 1 is not readily transmitted through the morsitans group flies, which are commonly kept as laboratory colonies. It is unlikely that T. b. gambiense group 2 isolates represent genetic hybrids of T. b. gambiense group 1 and T. b. brucei, although they have the human infectivity of the former and the virulence and fly transmissibility of the latter. T. b. gambiense group 2 isolates shared only a single microsatellite marker with sympatric group 1 isolates [8]. T. b. gambiense groups 1 and 2 are also unlikely to have the same mechanism of human serum resistance. We know that neither group possesses the SRA gene, which confers human infectivity on T. b. rhodesiense [15,17,25,26], but whereas T. b. gambiense group 1 shows solid resistance to human serum, human serum resistance in T. b. gambiense group 2 varies with parasite passage as in T. b. rhodesiense [27].

We already know that T. b. gambiense group 1 isolates have a restricted range of genetic polymorphisms and the smallest genomes within the T. brucei species complex [20,28]. But what level of divergence does this represent? Perhaps a genome-wide comparison of T. b. gambiense group 1 and T. b. brucei will provide an answer.

Species in subgenus Nannomonas

Trypanosomes of subgenus Nannomonas are defined by their developmental cycle in the tsetse fly, which involves the midgut and proboscis. As bloodstream forms these trypanosomes are the smallest of the Salivaria, but there is considerable morphological variation, both in dimensions (length and maximum width), and in features such as body shape, prominence of the undulating membrane and presence of a free flagellum [1]. Coupled with variation in host range and pathogenicity, this morphological variation led to the description of many species and variants in the past. However, these fine distinctions were later disregarded and traditionally subgenus Nannomonas is split into 2 species: Trypanosoma congolense, which has a wide range of ungulate hosts, and T. simiae, for which pigs are regarded as the most important host [1]. This simplifies clinical diagnosis: if you find a small trypanosome in the blood of a sick ox, goat or sheep, it will be T. congolense, while in pigs with acute trypanosomiasis it will be T. simiae.

This simple picture may be set to change. A number of hitherto cryptic subgroups have been discovered by molecular characterisation during the past 20 years or so. Only one of these has been described in sufficient detail to warrant acceptance as a new species, T. godfreyi [29]. Molecular characterisation reveals that T. congolense is divided into 3 subgroups, savannah, forest and kilifi (or Kenya coast), while the T. simiae group comprises T. simiae and T. simiae tsavo (Table 3) [29-35]. Each subgroup possesses a unique satellite DNA sequence, and these sequences have been exploited for the development of specific DNA probes and PCR tests [36-39]. The ability to identify these subgroups accurately opens the way for systematic studies of their host range, distribution, pathogenicity, etc. The satellite DNA repeats are sensitive tools for identification, because they are highly reiterated in the genome, forming the bulk of the minichromosomes. The equivalent satellite DNA repeat of T. brucei is conserved throughout subgenus Trypanozoon [40,41]. This suggests either that the satellite repeats evolve very rapidly in subgenus Nannomonas compared to subgenus Trypanozoon, or that divergence between subgroups in subgenus Nannomonas is greater than that between subdivisions in subgenus Trypanozoon.

Table 3.

Characteristics of species and genotypes within subgenus Nannomonas. See text for explanation

Species	Genotype	Distribution	Host range	Transmission – recorded high prevalence	Growth in rodents	Satellite DNA repeat size	KDNA minicircle size	Miniexon repeat size
Trypanosoma congolense	savannah	Tropical Africa	Wide range of ungulates & other mammals	morsitans, palpalis and fusca groups	+	369 bp	850 bp	760 – 1500 bp
	forest	West & Central Africa	Pigs, goats. cattle, dogs, Other?	palpalis group	+	~350 bp	850 bp	~800 bp
	kilifi or Kenya coast	East Africa	Cattle, sheep, goats. Not pigs Other?	morsitans group	+	368 bp	700 bp	~600 bp
Trypanosoma simiae		Tropical Africa	Suids	Morsitans, palpalis and fusca groups	-	521 bp	900 bp	630 bp
	tsavo	East Africa	Suids	morsitans and fusca groups	-	~540 bp	-	508 bp
Trypanosoma godfreyi		Tropical Africa	Suids	morsitans and fusca groups	-	373 bp	800, 750 bp	~500 bp

Few attempts have been made to assess the level of genetic divergence within subgenus Nannomonas. Total DNA hybridisation showed that T. congolense savannah and kilifi subgroups were only distantly related compared to species within subgenus Trypanozoon [42]. A survey of nuclear and kinetoplast DNA polymorphisms in 5 species/subgroups (T. congolense savannah, forest and kilifi, T. simiae, T. godfreyi) revealed differences in the size of miniexon repeats and kDNA minicircles and maxicircles (Table 3) [43]. In this study, the most closely related trypanosomes were T. congolense savannah and forest [43], which share 71% similarity in satellite DNA sequence compared to an average 40–45% similiarity in the rest of the subgenus [44]. The T. congolense kilifi subgroup was as divergent from other T. congolense subgroups as from T. simiae or T. godfreyi [43]. The gene for the major surface glycoprotein, glutamate and alanine rich protein or GARP, is well conserved among T. congolense subgroups; the amino acid sequences of T. congolense savannah and forest strains differed by 4–5%, compared to about 16% from kilifi subgroup [45]. More divergent GARP genes have also been identified in T. simiae and T. godfreyi [46]. In agreement with the variation seen in GARP genes, phylogenetic analysis based on the 18S ribosomal RNA gene divides subgenus Nannomonas into 2 major clades: (1) T. congolense savannah, forest and kilifi subgroups, (2) T. simiae, T. godfreyi and T. simiae tsavo (previously designated T. congolense tsavo [31,34]. The absolute nucleotide differences between 18S ribosomal RNA genes in subgenus Nannomonas are larger than those in subgenus Trypanozoon [34].

In summary, there is compelling molecular evidence of far greater levels of genetic divergence within subgenus Nannomonas compared to subgenus Trypanozoon. However, biological criteria to support these "molecular taxa" are scarce. The inability of several of these trypanosomes to grow in experimental rodents has precluded the isolation of bloodstream forms from mammalian hosts or tsetse mouthparts in the field, so host range and distribution data are incomplete (Table 3). The use of PCR identification of tsetse infections has led to recognition that some of these new genotypes are extremely widespread and prevalent in the field, e.g. [47-51]. What contribution do these trypanosomes make to livestock disease? Are these various genotypes responsible for assumed "strain" differences in drug response, virulence or fly transmission dynamics? Can these new genotypes be correlated with the old morphological criteria and species designations? We really need the biology to catch up with the molecular taxonomy to answer these questions.

Conclusions

There is no consistency in the way species and subspecies names are applied in subgenera Trypanozoon and Nannomonas. In subgenus Trypanozoon the taxonomic importance of pathogenicity, host range and distribution appear to have been inflated relative to actual levels of genetic divergence. Taking all evidence into account, it is arguable that T. b. gambiense group 1 should be reinstated as the species T. gambiense, leaving T. b. rhodesiense and T. b. gambiense group 2 as host range variants of T. b. brucei.

In comparison, current taxonomic usage badly underrepresents diversity in subgenus Nannomonas. Data from molecular characterisation are revealing a growing number of genotypes, which may represent distinct taxa. Unfortunately few of these genotypes are yet supported by sufficient biological data to be recognized taxonomically. But we may be missing fundamental epidemiological information, because of our inability to distinguish these trypanosomes in host blood morphologically or in tsetse by their developmental cycle. Molecular taxonomy has led the way in identifying these new genotypes and now offers the key to elucidating the biology of these organisms.

Competing interests

None declared.