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. 1993 May;61(5):1958–1963. doi: 10.1128/iai.61.5.1958-1963.1993

An approach to development of specific T-lymphocyte lines by use of preprocessed antigens in Plasmodium vinckei vinckei murine malaria.

G M Wasserman 1, S Kumar 1, J Ahlers 1, F Ramsdell 1, J A Berzofsky 1, L H Miller 1
PMCID: PMC280789  PMID: 8478085

Abstract

The development of parasite-specific T-cell lines represents one approach to the potential identification of relevant immunogens in erythrocytic malarial infection. However, the use of parasitized-erythrocyte lysates as antigens inhibits the proliferation of T cells. To circumvent this problem, we preincubated antigen-presenting cells (APCs) from spleens of malaria-naive, BALB/c mice with a Plasmodium vinckei vinckei (hereafter referred to as P. vinckei)-parasitized erythrocyte lysate. APCs were subsequently irradiated and washed prior to being incubated with T lymphocytes from P. vinckei-immune, histocompatible mice. After 8 to 10 cycles of antigenic stimulation and rest, two T-cell lines were analyzed. Both lines were predominantly CD4+. Proliferation assays demonstrated marked lymphocyte blastogenesis to syngeneic but not allogeneic APCs that had preprocessed malarial antigen. Antigen incubated directly with T cells and nonpulsed APCs in vitro did not result in T-cell proliferation. Assays of interleukin-2 (IL-2), IL-4, IL-5, and gamma interferon were compatible with one cell line being predominantly TH1 and the other being TH2. Thus, APCs that have preprocessed malarial antigen and are free of extraneous parasite material induce highly reactive, antigen-specific, major histocompatibility complex-restricted T-cell lines that functionally appear capable of inducing humoral and/or cell-mediated immunity.

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Selected References

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  1. Ben-Sasson S. Z., Le Gros G., Conrad D. H., Finkelman F. D., Paul W. E. Cross-linking Fc receptors stimulate splenic non-B, non-T cells to secrete interleukin 4 and other lymphokines. Proc Natl Acad Sci U S A. 1990 Feb;87(4):1421–1425. doi: 10.1073/pnas.87.4.1421. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Brake D. A., Long C. A., Weidanz W. P. Adoptive protection against Plasmodium chabaudi adami malaria in athymic nude mice by a cloned T cell line. J Immunol. 1988 Mar 15;140(6):1989–1993. [PubMed] [Google Scholar]
  3. Brake D. A., Weidanz W. P., Long C. A. Antigen-specific, interleukin 2-propagated T lymphocytes confer resistance to a murine malarial parasite, Plasmodium chabaudi adami. J Immunol. 1986 Jul 1;137(1):347–352. [PubMed] [Google Scholar]
  4. Capron A., Dessaint J. P. Molecular basis of host-parasite relationship: towards the definition of protective antigens. Immunol Rev. 1989 Dec;112:27–48. doi: 10.1111/j.1600-065x.1989.tb00551.x. [DOI] [PubMed] [Google Scholar]
  5. Chizzolini C., Geinoz A., Schrijvers D. Interleukin-2 reverses T cell unresponsiveness to Plasmodium falciparum-antigen in malaria immune subjects. Cell Immunol. 1990 Jun;128(1):1–10. doi: 10.1016/0008-8749(90)90001-8. [DOI] [PubMed] [Google Scholar]
  6. Corradin G. Antigen processing and presentation. Immunol Lett. 1990 Aug;25(1-3):11–13. doi: 10.1016/0165-2478(90)90082-2. [DOI] [PubMed] [Google Scholar]
  7. Cresswell P. Immunology: questions of presentation. Nature. 1990 Feb 15;343(6259):593–594. doi: 10.1038/343593a0. [DOI] [PubMed] [Google Scholar]
  8. Curry R. C., Kiener P. A., Spitalny G. L. A sensitive immunochemical assay for biologically active MuIFN-gamma. J Immunol Methods. 1987 Nov 23;104(1-2):137–142. doi: 10.1016/0022-1759(87)90497-2. [DOI] [PubMed] [Google Scholar]
  9. Dialynas D. P., Quan Z. S., Wall K. A., Pierres A., Quintáns J., Loken M. R., Pierres M., Fitch F. W. Characterization of the murine T cell surface molecule, designated L3T4, identified by monoclonal antibody GK1.5: similarity of L3T4 to the human Leu-3/T4 molecule. J Immunol. 1983 Nov;131(5):2445–2451. [PubMed] [Google Scholar]
  10. Dontfraid F., Cochran M. A., Pombo D., Knell J. D., Quakyi I. A., Kumar S., Houghten R. A., Berzofsky J. A., Miller L. H., Good M. F. Human and murine CD4 T cell epitopes map to the same region of the malaria circumsporozoite protein: limited immunogenicity of sporozoites and circumsporozoite protein. Mol Biol Med. 1988 Dec;5(3):185–196. [PubMed] [Google Scholar]
  11. Fernandez-Botran R., Sanders V. M., Mosmann T. R., Vitetta E. S. Lymphokine-mediated regulation of the proliferative response of clones of T helper 1 and T helper 2 cells. J Exp Med. 1988 Aug 1;168(2):543–558. doi: 10.1084/jem.168.2.543. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Good M. F., Miller L. H. Involvement of T cells in malaria immunity: implications for vaccine development. Vaccine. 1989 Feb;7(1):3–9. doi: 10.1016/0264-410x(89)90002-9. [DOI] [PubMed] [Google Scholar]
  13. Good M. F. T cells, T sites, and malaria immunity--further optimism for vaccine development. J Immunol. 1988 Mar 15;140(6):1715–1716. [PubMed] [Google Scholar]
  14. Goonewardene R., Carter R., Gamage C. P., Del Giudice G., David P. H., Howie S., Mendis K. N. Human T cell proliferative responses to Plasmodium vivax antigens: evidence of immunosuppression following prolonged exposure to endemic malaria. Eur J Immunol. 1990 Jun;20(6):1387–1391. doi: 10.1002/eji.1830200626. [DOI] [PubMed] [Google Scholar]
  15. Gross A., Frankenburg S. Plasmodium berghei: immunosuppression of the cell-mediated immune response induced by nonviable antigenic preparations. Exp Parasitol. 1989 Jan;68(1):83–92. doi: 10.1016/0014-4894(89)90011-8. [DOI] [PubMed] [Google Scholar]
  16. Havran W. L., Poenie M., Kimura J., Tsien R., Weiss A., Allison J. P. Expression and function of the CD3-antigen receptor on murine CD4+8+ thymocytes. Nature. 1987 Nov 12;330(6144):170–173. doi: 10.1038/330170a0. [DOI] [PubMed] [Google Scholar]
  17. Hu-Li J., Ohara J., Watson C., Tsang W., Paul W. E. Derivation of a T cell line that is highly responsive to IL-4 and IL-2 (CT.4R) and of an IL-2 hyporesponsive mutant of that line (CT.4S). J Immunol. 1989 Feb 1;142(3):800–807. [PubMed] [Google Scholar]
  18. Khansari N., Segre M., Segre D. Immunosuppression in murine malaria: a soluble immunosuppressive factor derived from Plasmodium berghei-infected blood. J Immunol. 1981 Nov;127(5):1889–1893. [PubMed] [Google Scholar]
  19. Kodama K., Kawamura S., Yasukawa M., Kobayashi Y. Establishment and characterization of a T-cell line specific for Rickettsia tsutsugamushi. Infect Immun. 1987 Oct;55(10):2490–2495. doi: 10.1128/iai.55.10.2490-2495.1987. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. Kumar S., Good M. F., Dontfraid F., Vinetz J. M., Miller L. H. Interdependence of CD4+ T cells and malarial spleen in immunity to Plasmodium vinckei vinckei. Relevance to vaccine development. J Immunol. 1989 Sep 15;143(6):2017–2023. [PubMed] [Google Scholar]
  21. Lamb J. R., Young D. B. A novel approach to the identification of T-cell epitopes in Mycobacterium tuberculosis using human T-lymphocyte clones. Immunology. 1987 Jan;60(1):1–5. [PMC free article] [PubMed] [Google Scholar]
  22. Langhorne J. The role of CD4+ T-cells in the immune response to Plasmodium chabaudi. Parasitol Today. 1989 Nov;5(11):362–364. doi: 10.1016/0169-4758(89)90113-0. [DOI] [PubMed] [Google Scholar]
  23. Loyola E. G., Rodriguez M. H., Gonzalez-Ceron L., Vaca-Marin M. A. Estimates of malaria incidence. Parasitol Today. 1989 Dec;5(12):384–384. doi: 10.1016/0169-4758(89)90301-3. [DOI] [PubMed] [Google Scholar]
  24. Miller L. H. Strategies for malaria control: realities, magic, and science. Ann N Y Acad Sci. 1989;569:118–126. doi: 10.1111/j.1749-6632.1989.tb27362.x. [DOI] [PubMed] [Google Scholar]
  25. Mosmann T. R., Bond M. W., Coffman R. L., Ohara J., Paul W. E. T-cell and mast cell lines respond to B-cell stimulatory factor 1. Proc Natl Acad Sci U S A. 1986 Aug;83(15):5654–5658. doi: 10.1073/pnas.83.15.5654. [DOI] [PMC free article] [PubMed] [Google Scholar]
  26. Mosmann T. R., Cherwinski H., Bond M. W., Giedlin M. A., Coffman R. L. Two types of murine helper T cell clone. I. Definition according to profiles of lymphokine activities and secreted proteins. J Immunol. 1986 Apr 1;136(7):2348–2357. [PubMed] [Google Scholar]
  27. Mustafa A. S., Gill H. K., Nerland A., Britton W. J., Mehra V., Bloom B. R., Young R. A., Godal T. Human T-cell clones recognize a major M. leprae protein antigen expressed in E. coli. Nature. 1986 Jan 2;319(6048):63–66. doi: 10.1038/319063a0. [DOI] [PubMed] [Google Scholar]
  28. Ohara J., Paul W. E. Production of a monoclonal antibody to and molecular characterization of B-cell stimulatory factor-1. Nature. 1985 May 23;315(6017):333–336. doi: 10.1038/315333a0. [DOI] [PubMed] [Google Scholar]
  29. Pierres A., Naquet P., Van Agthoven A., Bekkhoucha F., Denizot F., Mishal Z., Schmitt-Verhulst A. M., Pierres M. A rat anti-mouse T4 monoclonal antibody (H129.19) inhibits the proliferation of Ia-reactive T cell clones and delineates two phenotypically distinct (T4+, Lyt-2,3-, and T4-, Lyt-2,3+) subsets among anti-Ia cytolytic T cell clones. J Immunol. 1984 Jun;132(6):2775–2782. [PubMed] [Google Scholar]
  30. Riley E. M., Jobe O., Blackman M., Whittle H. C., Greenwood B. M. Plasmodium falciparum schizont sonic extracts suppress lymphoproliferative responses to mitogens and antigens in malaria-immune adults. Infect Immun. 1989 Oct;57(10):3181–3188. doi: 10.1128/iai.57.10.3181-3188.1989. [DOI] [PMC free article] [PubMed] [Google Scholar]
  31. Schumacher J. H., O'Garra A., Shrader B., van Kimmenade A., Bond M. W., Mosmann T. R., Coffman R. L. The characterization of four monoclonal antibodies specific for mouse IL-5 and development of mouse and human IL-5 enzyme-linked immunosorbent. J Immunol. 1988 Sep 1;141(5):1576–1581. [PubMed] [Google Scholar]
  32. Scott P., Caspar P., Sher A. Protection against Leishmania major in BALB/c mice by adoptive transfer of a T cell clone recognizing a low molecular weight antigen released by promastigotes. J Immunol. 1990 Feb 1;144(3):1075–1079. [PubMed] [Google Scholar]
  33. Scott P., Natovitz P., Coffman R. L., Pearce E., Sher A. Immunoregulation of cutaneous leishmaniasis. T cell lines that transfer protective immunity or exacerbation belong to different T helper subsets and respond to distinct parasite antigens. J Exp Med. 1988 Nov 1;168(5):1675–1684. doi: 10.1084/jem.168.5.1675. [DOI] [PMC free article] [PubMed] [Google Scholar]
  34. Simon-Haarhaus B., Langhorne J., Meding S. CD4+T cell-dependent effector mechanisms important in the immune response to the erythrocytic stages of Plasmodium chabaudi chabaudi (AS). Behring Inst Mitt. 1991 Feb;(88):94–98. [PubMed] [Google Scholar]
  35. Winkel K. D., Good M. F. Inability of Plasmodium vinckei-immune spleen cells to transfer protection to recipient mice exposed to vaccine 'vectors' or heterologous species of plasmodium. Parasite Immunol. 1991 Sep;13(5):517–530. doi: 10.1111/j.1365-3024.1991.tb00548.x. [DOI] [PubMed] [Google Scholar]
  36. Young D. B., Lamb J. R. T lymphocytes respond to solid-phase antigen: a novel approach to the molecular analysis of cellular immunity. Immunology. 1986 Oct;59(2):167–171. [PMC free article] [PubMed] [Google Scholar]

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