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. Author manuscript; available in PMC: 2010 Dec 29.
Published in final edited form as: Biochemistry. 2009 Dec 29;48(51):12345–12357. doi: 10.1021/bi9015555

Figure 4. Effect of low concentrations of DTT on (A) oligomerization and (B) oxidation states of adiponectin octadecamers using gel electrophoresis and confirmation of oligomerization state in solution by dynamic light scattering under (C) oxidized and (D) reduced conditions.

Figure 4

Purified adiponectin octadecamers in PBS were treated with or without 4 mM DTT at 4°C overnight. Oligomerization state was assessed using native and oxidation state using non-reducing denaturing PAGE. AMS was used to quench DTT and alkylate reduced cysteines to prevent their oxidation during sample denaturation. Samples identically treated as those loaded onto native gels were analyzed using dynamic light scattering as described in Experimental Procedures.