Skip to main content
PMC home page
Infection and Immunity logoLink to Infection and Immunity
. 1993 May;61(5):2211–2215. doi: 10.1128/iai.61.5.2211-2215.1993

Biochemical and immunological properties of two forms of pertactin, the 69,000-molecular-weight outer membrane protein of Bordetella pertussis.

J W Gotto 1, T Eckhardt 1, P A Reilly 1, J V Scott 1, J L Cowell 1, T N Metcalf 3rd 1, K Mountzouros 1, J J Gibbons Jr 1, M Siegel 1
PMCID: PMC280825  PMID: 8478113

Abstract

Two apparent isoforms of the virulence-associated 69,000-molecular-weight protein pertactin were purified from Bordetella pertussis. Mass spectrometry showed a difference of 2,060 Da, which may result from differential C-terminal cleavage of a larger precursor. Both forms were protective in a mouse model, eliciting bactericidal antibodies and reducing respiratory tract colonization.

Full text

PDF
2211

Images in this article

2212Image
on p.2212
2213Image
on p.2213

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Aoyama T., Murase Y., Gonda T., Iwata T. Type-specific efficacy of acellular pertussis vaccine. Am J Dis Child. 1988 Jan;142(1):40–42. doi: 10.1001/archpedi.1988.02150010050019. [DOI] [PubMed] [Google Scholar]
  2. Brennan M. J., Li Z. M., Cowell J. L., Bisher M. E., Steven A. C., Novotny P., Manclark C. R. Identification of a 69-kilodalton nonfimbrial protein as an agglutinogen of Bordetella pertussis. Infect Immun. 1988 Dec;56(12):3189–3195. doi: 10.1128/iai.56.12.3189-3195.1988. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Charles I. G., Dougan G., Pickard D., Chatfield S., Smith M., Novotny P., Morrissey P., Fairweather N. F. Molecular cloning and characterization of protective outer membrane protein P.69 from Bordetella pertussis. Proc Natl Acad Sci U S A. 1989 May;86(10):3554–3558. doi: 10.1073/pnas.86.10.3554. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Charles I. G., Li J. L., Roberts M., Beesley K., Romanos M., Pickard D. J., Francis M., Campbell D., Dougan G., Brennan M. J. Identification and characterization of a protective immunodominant B cell epitope of pertactin (P.69) from Bordetella pertussis. Eur J Immunol. 1991 May;21(5):1147–1153. doi: 10.1002/eji.1830210509. [DOI] [PubMed] [Google Scholar]
  5. Devereux J., Haeberli P., Smithies O. A comprehensive set of sequence analysis programs for the VAX. Nucleic Acids Res. 1984 Jan 11;12(1 Pt 1):387–395. doi: 10.1093/nar/12.1part1.387. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Kimura A., Mountzouros K. T., Relman D. A., Falkow S., Cowell J. L. Bordetella pertussis filamentous hemagglutinin: evaluation as a protective antigen and colonization factor in a mouse respiratory infection model. Infect Immun. 1990 Jan;58(1):7–16. doi: 10.1128/iai.58.1.7-16.1990. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Leininger E., Roberts M., Kenimer J. G., Charles I. G., Fairweather N., Novotny P., Brennan M. J. Pertactin, an Arg-Gly-Asp-containing Bordetella pertussis surface protein that promotes adherence of mammalian cells. Proc Natl Acad Sci U S A. 1991 Jan 15;88(2):345–349. doi: 10.1073/pnas.88.2.345. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Makoff A. J., Oxer M. D., Ballantine S. P., Fairweather N. F., Charles I. G. Protective surface antigen P69 of Bordetella pertussis: its characterization and very high level expression in Escherichia coli. Biotechnology (N Y) 1990 Nov;8(11):1030–1033. doi: 10.1038/nbt1190-1030. [DOI] [PubMed] [Google Scholar]
  9. Montaraz J. A., Novotny P., Ivanyi J. Identification of a 68-kilodalton protective protein antigen from Bordetella bronchiseptica. Infect Immun. 1985 Mar;47(3):744–751. doi: 10.1128/iai.47.3.744-751.1985. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Mortimer E. A., Jr, Kimura M., Cherry J. D., Kuno-Sakai H., Stout M. G., Dekker C. L., Hayashi R., Miyamoto Y., Scott J. V., Aoyama T. Protective efficacy of the Takeda acellular pertussis vaccine combined with diphtheria and tetanus toxoids following household exposure of Japanese children. Am J Dis Child. 1990 Aug;144(8):899–904. doi: 10.1001/archpedi.1990.02150320063029. [DOI] [PubMed] [Google Scholar]
  11. Novotny P., Chubb A. P., Cownley K., Charles I. G. Biologic and protective properties of the 69-kDa outer membrane protein of Bordetella pertussis: a novel formulation for an acellular pertussis vaccine. J Infect Dis. 1991 Jul;164(1):114–122. doi: 10.1093/infdis/164.1.114. [DOI] [PubMed] [Google Scholar]
  12. Novotny P., Chubb A. P., Cownley K., Montaraz J. A. Adenylate cyclase activity of a 68,000-molecular-weight protein isolated from the outer membrane of Bordetella bronchiseptica. Infect Immun. 1985 Oct;50(1):199–206. doi: 10.1128/iai.50.1.199-206.1985. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Novotny P., Chubb A. P., Cownley K., Montaraz J. A., Beesley J. E. Bordetella adenylate cyclase: a genus specific protective antigen and virulence factor. Dev Biol Stand. 1985;61:27–41. [PubMed] [Google Scholar]
  14. Roberts M., Maskell D., Novotny P., Dougan G. Construction and characterization in vivo of Bordetella pertussis aroA mutants. Infect Immun. 1990 Mar;58(3):732–739. doi: 10.1128/iai.58.3.732-739.1990. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Shahin R. D., Brennan M. J., Li Z. M., Meade B. D., Manclark C. R. Characterization of the protective capacity and immunogenicity of the 69-kD outer membrane protein of Bordetella pertussis. J Exp Med. 1990 Jan 1;171(1):63–73. doi: 10.1084/jem.171.1.63. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Siegel M. M., Hollander I. J., Hamann P. R., James J. P., Hinman L., Smith B. J., Farnsworth A. P., Phipps A., King D. J., Karas M. Matrix-assisted UV-laser desorption/ionization mass spectrometric analysis of monoclonal antibodies for the determination of carbohydrate, conjugated chelator, and conjugated drug content. Anal Chem. 1991 Nov 1;63(21):2470–2481. doi: 10.1021/ac00021a016. [DOI] [PubMed] [Google Scholar]
  17. Stainer D. W., Scholte M. J. A simple chemically defined medium for the production of phase I Bordetella pertussis. J Gen Microbiol. 1970 Oct;63(2):211–220. doi: 10.1099/00221287-63-2-211. [DOI] [PubMed] [Google Scholar]
  18. Stibitz S., Weiss A. A., Falkow S. Genetic analysis of a region of the Bordetella pertussis chromosome encoding filamentous hemagglutinin and the pleiotropic regulatory locus vir. J Bacteriol. 1988 Jul;170(7):2904–2913. doi: 10.1128/jb.170.7.2904-2913.1988. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Weiss A. A., Falkow S. Genetic analysis of phase change in Bordetella pertussis. Infect Immun. 1984 Jan;43(1):263–269. doi: 10.1128/iai.43.1.263-269.1984. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from Infection and Immunity are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES