Abstract
The major outer membrane protein of Haemophilus somnus possesses at least five distinct epitopes. Three surface-exposed epitopes on the major outer membrane protein include a conserved epitope with potential for development of a vaccine and a diagnostic test and two variable epitopes responsible for antigenic differences among strains; the remaining two epitopes are well preserved among strains but not exposed on the cell surface.
Full text
PDF


Images in this article
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- Corbeil L. B., Arthur J. E., Widders P. R., Smith J. W., Barbet A. F. Antigenic specificity of convalescent serum from cattle with haemophilus somnus-induced experimental abortion. Infect Immun. 1987 Jun;55(6):1381–1386. doi: 10.1128/iai.55.6.1381-1386.1987. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Dooley J. S., Trust T. J. Surface protein composition of Aeromonas hydrophila strains virulent for fish: identification of a surface array protein. J Bacteriol. 1988 Feb;170(2):499–506. doi: 10.1128/jb.170.2.499-506.1988. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Haase E. M., Campagnari A. A., Sarwar J., Shero M., Wirth M., Cumming C. U., Murphy T. F. Strain-specific and immunodominant surface epitopes of the P2 porin protein of nontypeable Haemophilus influenzae. Infect Immun. 1991 Apr;59(4):1278–1284. doi: 10.1128/iai.59.4.1278-1284.1991. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Hamel J., Brodeur B. R., Larose Y., Tsang P. S., Belmaaza A., Montplaisir S. A monoclonal antibody directed against a serotype-specific, outer-membrane protein of Haemophilus influenzae type b. J Med Microbiol. 1987 Mar;23(2):163–170. doi: 10.1099/00222615-23-2-163. [DOI] [PubMed] [Google Scholar]
- Laemmli U. K. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature. 1970 Aug 15;227(5259):680–685. doi: 10.1038/227680a0. [DOI] [PubMed] [Google Scholar]
- Leong M. M., Fox G. R. Luminescent detection of immunodot and western blots. Methods Enzymol. 1990;184:442–451. [PubMed] [Google Scholar]
- Munson R. S., Jr, Shenep J. L., Barenkamp S. J., Granoff D. M. Purification and comparison of outer membrane protein P2 from Haemophilus influenzae type b isolates. J Clin Invest. 1983 Aug;72(2):677–684. doi: 10.1172/JCI111017. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Murphy T. F., Bartos L. C. Purification and analysis with monoclonal antibodies of P2, the major outer membrane protein of nontypable Haemophilus influenzae. Infect Immun. 1988 May;56(5):1084–1089. doi: 10.1128/iai.56.5.1084-1089.1988. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Robertson S. M., Frisch C. F., Gulig P. A., Kettman J. R., Johnston K. H., Hansen E. J. Monoclonal antibodies directed against a cell surface-exposed outer membrane protein of Haemophilus influenzae type b. Infect Immun. 1982 Apr;36(1):80–88. doi: 10.1128/iai.36.1.80-88.1982. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Tagawa Y., Haritani M., Ishikawa H., Yuasa N. Characterization of a heat-modifiable outer membrane protein of Haemophilus somnus. Infect Immun. 1993 May;61(5):1750–1755. doi: 10.1128/iai.61.5.1750-1755.1993. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Tagawa Y., Ishikawa H., Yuasa N. Purification and partial characterization of the major outer membrane protein of Haemophilus somnus. Infect Immun. 1993 Jan;61(1):91–96. doi: 10.1128/iai.61.1.91-96.1993. [DOI] [PMC free article] [PubMed] [Google Scholar]