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. 1993 Jun;61(6):2558–2562. doi: 10.1128/iai.61.6.2558-2562.1993

Specific antibody to Haemophilus somnus in the bovine uterus following intramuscular immunization.

B M Butt 1, T E Besser 1, P L Senger 1, P R Widders 1
PMCID: PMC280884  PMID: 8500892

Abstract

Sources of anti-Haemophilus somnus antibody in bovine uterine secretions following intramuscular immunization and subsequent intrauterine inoculation of killed H. somnus were investigated. Holstein cattle (n = 21) were immunized with a 270-kDa outer membrane protein from H. somnus (omp-270) by intramuscular injection. At estrus, the cattle were given an intrauterine inoculum of a heat-killed suspension of a homologous strain of H. somnus containing omp-270 (n = 7), a heterologous strain of H. somnus lacking omp-270 (n = 7), or phosphate-buffered saline (n = 7). Uterine secretions were sampled by saline lavage immediately prior to inoculation and at 6, 24, 48, 72, 96, and 120 h after inoculation. Immunoglobulin G subclass I (IgG1) and IgG2 antibody specific for omp-270 were detectable in estrous uterine secretions of all systemically immunized cattle from which an adequate sample was obtained. IgM antibody specific for omp-270 was detected in serum following immunization but was not consistently detected in the uterine secretions of any animal. IgA antibody specific for omp-270 was not detectable in either serum or uterine secretions following immunization or intrauterine inoculation. Ratios of antibody to immunoglobulin and ratios of immunoglobulin to albumin in serum and uterine secretions indicated that about half the IgG1 and essentially all the IgG2 in secretions originated in the serum. Relative titers of IgG1 and IgG2 omp-270-specific antibodies in the uterine lumen and serum gave no evidence for selective transport of either subclass from serum into local secretions. Neither heterologous nor homologous intrauterine inocula detectably altered the serum contribution to antibody in uterine secretions within the sampling period. On the basis of these results, development of a systemic IgG2 antibody response may provide the basis for local immunological protection in the bovine reproductive tract.

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Selected References

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  1. Anderson K. L., Hemeida N. A., Frank A., Whitmore H. L., Gustafsson B. K. Collection and phagocytic evaluation of uterine neutrophilic leukocytes. Theriogenology. 1985 Sep;24(3):305–317. doi: 10.1016/0093-691x(85)90221-3. [DOI] [PubMed] [Google Scholar]
  2. Butler J. E. Bovine immunoglobulins: an augmented review. Vet Immunol Immunopathol. 1983 Mar;4(1-2):43–152. doi: 10.1016/0165-2427(83)90056-9. [DOI] [PubMed] [Google Scholar]
  3. Butt B. M., Senger P. L., Widders P. R. Neutrophil migration into the bovine uterine lumen following intrauterine inoculation with killed Haemophilus somnus. J Reprod Fertil. 1991 Nov;93(2):341–345. doi: 10.1530/jrf.0.0930341. [DOI] [PubMed] [Google Scholar]
  4. Corbeil L. B., Duncan J. R., Schurig G. G., Hall C. E., Winter A. J. Bovine venereal vibriosis: variations in immunoglobulin class of antibodies in genital secretions and serum. Infect Immun. 1974 Nov;10(5):1084–1090. doi: 10.1128/iai.10.5.1084-1090.1974. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Corbeil L. B., Schurig G. D., Duncan J. R., Corbeil R. R., Winter A. J. Immunoglobulin classes and biological functions of Campylobacter (Vibrio) fetus antibodies in serum and cervicovaginal mucus. Infect Immun. 1974 Sep;10(3):422–429. doi: 10.1128/iai.10.3.422-429.1974. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Corbeil L. B., Schurig G. G., Duncan J. R., Wilkie B. N., Winter A. J. Immunity in the female bovine reproductive tract based on the response to "Campylobacter fetus". Adv Exp Med Biol. 1981;137:729–743. [PubMed] [Google Scholar]
  7. Howard C. J., Taylor G., Brownlie J. Surface receptors for immunoglobulin on bovine polymorphonuclear neutrophils and macrophages. Res Vet Sci. 1980 Jul;29(1):128–130. [PubMed] [Google Scholar]
  8. McDermott M. R., Clark D. A., Bienenstock J. Evidence for a common mucosal immunologic system. II. Influence of the estrous cycle on B immunoblast migration into genital and intestinal tissues. J Immunol. 1980 Jun;124(6):2536–2539. [PubMed] [Google Scholar]
  9. McGuire T. C., Musoke A. J., Kurtti T. Functional properties of bovine IgG1 and IgG2: interaction with complement, macrophages, neutrophils and skin. Immunology. 1979 Oct;38(2):249–256. [PMC free article] [PubMed] [Google Scholar]
  10. SKJERVEN O. Endometrial biopsy studies in reproductively normal cattle; clinical, histochemical and histological observations during the estrous cycle. Acta Endocrinol Suppl (Copenh) 1956;22(Suppl 26):1–101. [PubMed] [Google Scholar]
  11. Schurig G. G., Duncan J. R., Winter A. J. Elimination of genital vibriosis in female cattle by systemic immunization with killed cells or cell-free extracts of Campylobacter fetus. J Infect Dis. 1978 Oct;138(4):463–472. doi: 10.1093/infdis/138.4.463. [DOI] [PubMed] [Google Scholar]
  12. Schurig G. G., Hall C. E., Corbell L. B., Duncan J. R., Winter A. J. Bovine veneral vibriosis: cure of genital infection in females by systemic immunization. Infect Immun. 1975 Feb;11(2):245–251. doi: 10.1128/iai.11.2.245-251.1975. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Targowski S. P. Immune-mediated migration of neutrophils into the uterine lumen of guinea pigs. Am J Reprod Immunol. 1984 Dec;6(4):190–194. doi: 10.1111/j.1600-0897.1984.tb00136.x. [DOI] [PubMed] [Google Scholar]
  14. Watson E. D., Diehl N. K., Evans J. F. Antibody response in the bovine genital tract to intrauterine infusion of Actinomyces pyogenes. Res Vet Sci. 1990 Jan;48(1):70–75. [PubMed] [Google Scholar]
  15. Widders P. R., Dorrance L. A., Yarnall M., Corbeil L. B. Immunoglobulin-binding activity among pathogenic and carrier isolates of Haemophilus somnus. Infect Immun. 1989 Feb;57(2):639–642. doi: 10.1128/iai.57.2.639-642.1989. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Widders P. R., Smith J. W., Yarnall M., McGuire T. C., Corbeil L. B. Non-immune immunoglobulin binding by "Haemophilus somnus". J Med Microbiol. 1988 Aug;26(4):307–311. doi: 10.1099/00222615-26-4-307. [DOI] [PubMed] [Google Scholar]
  17. Yarnall M., Gogolewski R. P., Corbeil L. B. Characterization of two Haemophilus somnus Fc receptors. J Gen Microbiol. 1988 Jul;134(7):1993–1999. doi: 10.1099/00221287-134-7-1993. [DOI] [PubMed] [Google Scholar]

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