Table 1.
Ability of mutant receptors to stimulate cAMP production.
| Mutant | N | pEC50 WT | pEC50 mutant | Mutant | N | pEC50 WT | pEC50 mutant |
|---|---|---|---|---|---|---|---|
| E23A | 4 | 8.98 ± 0.32 | 9.18 ± 0.37 | M42A | 6 | 9.81 ± 0.26 | 9.86 ± 0.10 |
| L24A | 4 | 9.09 ± 0.35 | 9.39 ± 0.42 | T43A | 3 | 9.77 ± 0.41 | 9.74 ± 0.37 |
| E25A | 6 | 10.08 ± 0.40 | 9.72 ± 0.39 | A44L | 5 | 9.32 ± 0.20 | 8.41 ± 0.45 |
| E26A | 5 | 9.33 ± 0.57 | 9.33 ± 0.21 | Q45A | 3 | 9.11 ± 0.08 | 8.10 ± 0.18** |
| S27A | 4 | 9.26 ± 0.14 | 9.51 ± 0.34 | Y46A | 4 | 9.17 ± 0.19 | 9.12 ± 0.44 |
| P28A | 4 | 9.11 ± 0.33 | 9.90 ± 0.37 | E47A | 5 | 9.57 ± 0.21 | 9.40 ± 0.32 |
| E29A | 4 | 9.41 ± 0.14 | 9.26 ± 0.20 | C48A | 7 | 10.09 ± 0.23 | 8.77 ± 0.38** |
| D30A | 4 | 9.66 ± 0.29 | 9.68 ± 0.41 | Y49A | 6 | 9.71 ± 0.19 | 8.76 ± 0.18** |
| S31A | 7 | 9.11 ± 0.31 | 9.00 ± 0.37 | Q50A | 5 | 9.49 ± 0.10 | 9.61 ± 0.49 |
| I32A | 4 | 8.95 ± 0.14 | 9.63 ± 0.13* | K51A | 4 | 9.45 ± 0.37 | 9.66 ± 0.25 |
| Q33A | 3 | 9.28 ± 0.22 | 9.04 ± 0.03 | I52A | 6 | 9.79 ± 0.24 | 9.86 ± 0.29 |
| L34A | 7 | 9.55 ± 0.30 | 9.51 ± 0.23 | M53A | 5 | 9.72 ± 0.30 | 10.20 ± 0.24 |
| G35A | 6 | 9.07 ± 0.12 | 10.09 ± 0.24** | Q54A | 4 | 9.38 ± 0.15 | 9.60 ± 0.33 |
| V36A | 4 | 9.27 ± 0.18 | 9.90 ± 0.30 | D55A | 5 | 9.33 ± 0.30 | 9.76 ± 0.13 |
| T37A | 5 | 9.15 ± 0.17 | 10.08 ± 0.23* | P56A | 3 | 9.74 ± 0.14 | 9.21 ± 0.18 |
| R38A | 5 | 9.17 ± 0.15 | 9.32 ± 0.35 | I57A | 3 | 9.23 ± 0.27 | 9.27 ± 0.33 |
| N39A | 4 | 9.24 ± 0.34 | 9.55 ± 0.42 | Q58A | 6 | 10.12 ± 0.36 | 10.28 ± 0.37 |
| K40A | 4 | 9.61 ± 0.15 | 10.01 ± 0.25 | Q59A | 5 | 9.68 ± 0.26 | 9.70 ± 0.32 |
| I41A | 7 | 10.02 ± 0.34 | 9.22 ± 0.096* | A60L | 6 | 9.65 ± 0.44 | 9.56 ± 0.43 |
Values are means ± s.e.m. *, **, p > 0.05 and 0.01 compared to WT, Student's, t-test.