Table 2.
Cell surface expression and binding properties of mutant receptors.
| Mutant | % Expression | % Binding | Mutant | % Expression | % Binding |
|---|---|---|---|---|---|
| E23A | 108.7 ± 5.1 | 81.7 ± 4.0 | M42A | 83.2 ± 6.3 | 97.2 ± 12.0 |
| L24A | 111.1 ± 9.2 | 101.6 ± 3.5 | T43A | 83.6 ± 5.3* | 87.6 ± 12.3 |
| E25A | 143.0 ± 16.4* | 90.6 ± 12.5 | A44L | 82.7 ± 6.0* | 4.3 ± 1.6* |
| E26A | 123.7 ± 8.6 | 71.9 ± 10 | Q45A | 66.6 ± 3.3*** | 61.4 ± 3.7 |
| S27A | 117.1 ± 7.5 | 116.4 ± 22.2 | Y46A | 167.0 ± 8.6*** | 71.4 ± 16.7 |
| P28A | 95.9 ± 9.9 | 90.2 ± 14 | E47A | 81.7 ± 4.7 ** | 90 ± 10.7 |
| E29A | 108.6 ± 9.7 | 90.5 ± 0.8 | C48A | 45.4 ± 3.9*** | 2.7 ± 1.2* |
| D30A | 123.4 ± 6.3** | 74.1 ± 5 | Y49A | 33.8 ± 5.6*** | 5.5 ± 3.1* |
| S31A | 111.4 ± 9.7 | 98.6 ± 2.4 | Q50A | 98.5 ± 9.4 | 93.5 ± 2.5 |
| I32A | 114.3 ± 9.9 | 114.7 ± 13.7 | K51A | 82.0 ± 8.4 | 85.9 ± 6.7 |
| Q33A | 141.0 ± 8.3*** | 59.2 ± 14.2 | I52A | 123.9 ± 5.8* | 86.2 ± 1.9 |
| L34A | 129.4 ± 14.6 | 103 ± 14.2 | M53A | 109.6 ± 5.1 | 95.8 ± 6.7 |
| G35A | 147.6 ± 9.2** | 103 ± 8.4 | Q54A | 106.5 ± 9.3 | 103.1 ± 15.8 |
| V36A | 116.1 ± 9.3* | 87.8 ± 6 | D55A | 100.5 ± 7.9 | 86.7 ± 10.4 |
| T37A | 108.5 ± 8.6 | 88.3 ± 8.1 | P56A | 131.1 ± 7.0** | 83.3 ± 8.8 |
| R38A | 161.7 ± 9.0*** | 89.0 ± 8.6 | I57A | 105.4 ± 9.9 | 102.1 ± 4.3 |
| N39A | 147.6 ± 10.0** | 94.9 ± 13.5 | Q58A | 88.0 ± 10.6 | 78.8 ± 7.9 |
| K40A | 83.9 ± 4.0 | 91.1 ± 9.0 | Q59A | 102.9 ± 7.9 | 82.1 ± 6.6 |
| I41A | 133.6 ± 8.7 | 2.0 ± 2.7 | A60L | 79.4 ± 7.1 | 104.9 ± 6 |
Expression shows cell surface expression of HA-tagged receptors, 3–6 experiments; WT expression = 100%. *, **, ***, p > 0.05, 0.01 and 0.001 compared to WT, Mann–Whitney. Binding shows % specific binding of 125I-CGRP; WT receptor binding = 100%, n = 3. Values are means ± s.e.m. *, **, ***, p > 0.05, 0.01 and 0.001 compared to WT, Student's t-test.