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. 1993 Jul;61(7):3064–3067. doi: 10.1128/iai.61.7.3064-3067.1993

Presentation of T-cell epitopes assembled as multiple-antigen peptides to murine and human T lymphocytes.

D Grillot 1, D Valmori 1, P H Lambert 1, G Corradin 1, G Del Giudice 1
PMCID: PMC280960  PMID: 7685741

Abstract

Multiple-antigen peptide (MAP) constructs containing different T- and B-cell epitopes were assessed for their ability to be specifically recognized by murine and human T-cell clones. The different synthetic MAP constructs consisted of a malaria T-cell epitope or of a human universal tetanus toxin helper T-cell epitope collinearly synthesized with B-cell epitopes from the circumsporozoite proteins of different malaria parasites. All constructs were able to stimulate specifically T-cell clones. Interestingly, T-cell epitopes assembled as MAP constructs did not require processing for the specific stimulation of murine and human T-cell clones, as shown by retention of their stimulatory effect in the presence of glutaraldehyde-fixed antigen-presenting cells. However, processing was required for most of the synthetic constructs containing both T- and B-cell epitopes. Thus, the requirement for processing of these constructs seems to be dictated by the nature of the B-cell epitope present.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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