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. 1993 Aug;61(8):3143–3148. doi: 10.1128/iai.61.8.3143-3148.1993

Alterations of bacterial clearance induced by endotoxin and tumor necrosis factor.

T Koch 1, H P Duncker 1, R Axt 1, H G Schiefer 1, K van Ackern 1, H Neuhof 1
PMCID: PMC280981  PMID: 8335345

Abstract

The purpose of the study was to investigate the potential influence of endotoxin and tumor necrosis factor (TNF) on immune function in terms of systemic clearance and organ distribution of injected Escherichia coli in a rabbit model. To enable quantification of the clearance process, defined numbers of exogenous E. coli (1.3 x 10(8) CFU) were injected intravenously 60 min after bolus application of TNF (4 x 10(5) U, n = 6), after infusion of endotoxin (40 micrograms/kg of body weight) for 1 h (n = 6) or 4 h (n = 6), or after saline infusion (controls, n = 6). Parameters monitored were arterial pressure, oxygen uptake, and rates of bacterial elimination from the blood. At 180 min after E. coli injection, the animals were sacrificed, and tissue samples of liver, kidney, spleen, and lung were collected for bacterial counts. Endotoxin infusion produced a significant delay in blood clearance compared with saline and TNF pretreatment. The diminished systemic bacterial elimination was associated with significantly higher numbers of E. coli in the organs, thus reflecting reticuloendothelial system dysfunction. TNF had no major influence on the elimination kinetics of bacteria but affected the tissue distribution pattern with increased accumulation of E. coli in the lung (up to 100-fold of control values; P < 0.001).

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Selected References

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  1. Abraham E., Chang Y. H. Effects of hemorrhage on inflammatory response. Arch Surg. 1984 Oct;119(10):1154–1157. doi: 10.1001/archsurg.1984.01390220040009. [DOI] [PubMed] [Google Scholar]
  2. Bachwich P. R., Chensue S. W., Larrick J. W., Kunkel S. L. Tumor necrosis factor stimulates interleukin-1 and prostaglandin E2 production in resting macrophages. Biochem Biophys Res Commun. 1986 Apr 14;136(1):94–101. doi: 10.1016/0006-291x(86)90881-8. [DOI] [PubMed] [Google Scholar]
  3. Baker J. W., Deitch E. A., Li M., Berg R. D., Specian R. D. Hemorrhagic shock induces bacterial translocation from the gut. J Trauma. 1988 Jul;28(7):896–906. doi: 10.1097/00005373-198807000-00002. [DOI] [PubMed] [Google Scholar]
  4. Beutler B. A., Milsark I. W., Cerami A. Cachectin/tumor necrosis factor: production, distribution, and metabolic fate in vivo. J Immunol. 1985 Dec;135(6):3972–3977. [PubMed] [Google Scholar]
  5. Carrico C. J., Meakins J. L., Marshall J. C., Fry D., Maier R. V. Multiple-organ-failure syndrome. Arch Surg. 1986 Feb;121(2):196–208. doi: 10.1001/archsurg.1986.01400020082010. [DOI] [PubMed] [Google Scholar]
  6. Chang S. W., Feddersen C. O., Henson P. M., Voelkel N. F. Platelet-activating factor mediates hemodynamic changes and lung injury in endotoxin-treated rats. J Clin Invest. 1987 May;79(5):1498–1509. doi: 10.1172/JCI112980. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Dayer J. M., Beutler B., Cerami A. Cachectin/tumor necrosis factor stimulates collagenase and prostaglandin E2 production by human synovial cells and dermal fibroblasts. J Exp Med. 1985 Dec 1;162(6):2163–2168. doi: 10.1084/jem.162.6.2163. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Deitch E. A., Berg R., Specian R. Endotoxin promotes the translocation of bacteria from the gut. Arch Surg. 1987 Feb;122(2):185–190. doi: 10.1001/archsurg.1987.01400140067008. [DOI] [PubMed] [Google Scholar]
  9. Deitch E. A., Bridges R. M. Effect of stress and trauma on bacterial translocation from the gut. J Surg Res. 1987 May;42(5):536–542. doi: 10.1016/0022-4804(87)90029-1. [DOI] [PubMed] [Google Scholar]
  10. Deitch E. A. Intestinal permeability is increased in burn patients shortly after injury. Surgery. 1990 Apr;107(4):411–416. [PubMed] [Google Scholar]
  11. Deitch E. A., Ma L., Ma W. J., Grisham M. B., Granger D. N., Specian R. D., Berg R. D. Inhibition of endotoxin-induced bacterial translocation in mice. J Clin Invest. 1989 Jul;84(1):36–42. doi: 10.1172/JCI114164. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Deitch E. A., Morrison J., Berg R., Specian R. D. Effect of hemorrhagic shock on bacterial translocation, intestinal morphology, and intestinal permeability in conventional and antibiotic-decontaminated rats. Crit Care Med. 1990 May;18(5):529–536. doi: 10.1097/00003246-199005000-00014. [DOI] [PubMed] [Google Scholar]
  13. Deitch E. A., Taylor M., Grisham M., Ma L., Bridges W., Berg R. Endotoxin induces bacterial translocation and increases xanthine oxidase activity. J Trauma. 1989 Dec;29(12):1679–1683. doi: 10.1097/00005373-198912000-00017. [DOI] [PubMed] [Google Scholar]
  14. FINE J., RUTENBURG S., SCHWEINBURG F. B. The role of the reticulo-endothelial system in hemorrhagic shock. J Exp Med. 1959 Oct 1;110:547–569. doi: 10.1084/jem.110.4.547. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Fink M. P., Gardiner M., MacVittie T. J. Sublethal hemorrhage impairs the acute peritoneal inflammatory response in the rat. J Trauma. 1985 Mar;25(3):234–237. doi: 10.1097/00005373-198503000-00013. [DOI] [PubMed] [Google Scholar]
  16. Girardin E., Grau G. E., Dayer J. M., Roux-Lombard P., Lambert P. H. Tumor necrosis factor and interleukin-1 in the serum of children with severe infectious purpura. N Engl J Med. 1988 Aug 18;319(7):397–400. doi: 10.1056/NEJM198808183190703. [DOI] [PubMed] [Google Scholar]
  17. Haranaka K., Carswell E. A., Williamson B. D., Prendergast J. S., Satomi N., Old L. J. Purification, characterization, and antitumor activity of nonrecombinant mouse tumor necrosis factor. Proc Natl Acad Sci U S A. 1986 Jun;83(11):3949–3953. doi: 10.1073/pnas.83.11.3949. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Hesse D. G., Tracey K. J., Fong Y., Manogue K. R., Palladino M. A., Jr, Cerami A., Shires G. T., Lowry S. F. Cytokine appearance in human endotoxemia and primate bacteremia. Surg Gynecol Obstet. 1988 Feb;166(2):147–153. [PubMed] [Google Scholar]
  19. Kaplan J. E., Saba T. M. Humoral deficiency and reticuloendothelial depression after traumatic shock. Am J Physiol. 1976 Jan;230(1):7–14. doi: 10.1152/ajplegacy.1976.230.1.7. [DOI] [PubMed] [Google Scholar]
  20. Klebanoff S. J., Vadas M. A., Harlan J. M., Sparks L. H., Gamble J. R., Agosti J. M., Waltersdorph A. M. Stimulation of neutrophils by tumor necrosis factor. J Immunol. 1986 Jun 1;136(11):4220–4225. [PubMed] [Google Scholar]
  21. Morris S. E., Navaratnam N., Herndon D. N. A comparison of effects of thermal injury and smoke inhalation on bacterial translocation. J Trauma. 1990 Jun;30(6):639–645. [PubMed] [Google Scholar]
  22. Morrison D. C., Ryan J. L. Bacterial endotoxins and host immune responses. Adv Immunol. 1979;28:293–450. doi: 10.1016/s0065-2776(08)60802-0. [DOI] [PubMed] [Google Scholar]
  23. Morrison D. C., Ryan J. L. Endotoxins and disease mechanisms. Annu Rev Med. 1987;38:417–432. doi: 10.1146/annurev.me.38.020187.002221. [DOI] [PubMed] [Google Scholar]
  24. Moxey-Mims M. M., Simms H. H., Frank M. M., Lin E. Y., Gaither T. A. The effects of IL-1, IL-2, and tumor necrosis factor on polymorphonuclear leukocyte Fc gamma receptor-mediated phagocytosis. IL-2 down-regulates the effect of tumor necrosis factor. J Immunol. 1991 Sep 15;147(6):1823–1830. [PubMed] [Google Scholar]
  25. Nawroth P. P., Bank I., Handley D., Cassimeris J., Chess L., Stern D. Tumor necrosis factor/cachectin interacts with endothelial cell receptors to induce release of interleukin 1. J Exp Med. 1986 Jun 1;163(6):1363–1375. doi: 10.1084/jem.163.6.1363. [DOI] [PMC free article] [PubMed] [Google Scholar]
  26. O'Dwyer S. T., Michie H. R., Ziegler T. R., Revhaug A., Smith R. J., Wilmore D. W. A single dose of endotoxin increases intestinal permeability in healthy humans. Arch Surg. 1988 Dec;123(12):1459–1464. doi: 10.1001/archsurg.1988.01400360029003. [DOI] [PubMed] [Google Scholar]
  27. Penn R. L., Maca R. D., Berg R. D. Increased translocation of bacteria from the gastrointestinal tracts of tumor-bearing mice. Infect Immun. 1985 Mar;47(3):793–798. doi: 10.1128/iai.47.3.793-798.1985. [DOI] [PMC free article] [PubMed] [Google Scholar]
  28. Rush B. F., Jr, Sori A. J., Murphy T. F., Smith S., Flanagan J. J., Jr, Machiedo G. W. Endotoxemia and bacteremia during hemorrhagic shock. The link between trauma and sepsis? Ann Surg. 1988 May;207(5):549–554. doi: 10.1097/00000658-198805000-00009. [DOI] [PMC free article] [PubMed] [Google Scholar]
  29. SANDYS G. H. A new method of preventing swarming of Proteus sp. with a description of a new medium suitable for use in routine laboratory practice. J Med Lab Technol. 1960 Oct;17:224–233. [PubMed] [Google Scholar]
  30. Saba T. M., Antikatzides T. G. Heparin induced alterations in clearance and distribution of blood-borne microparticles following operative trauma. Ann Surg. 1979 Apr;189(4):426–432. [PMC free article] [PubMed] [Google Scholar]
  31. Saba T. M. Effect of surgical trauma on the clearance and localization of blood-borne particulate matter. Surgery. 1972 May;71(5):675–685. [PubMed] [Google Scholar]
  32. Sun X. M., Hsueh W. Bowel necrosis induced by tumor necrosis factor in rats is mediated by platelet-activating factor. J Clin Invest. 1988 May;81(5):1328–1331. doi: 10.1172/JCI113459. [DOI] [PMC free article] [PubMed] [Google Scholar]
  33. Swan K. G., Reynolds D. G. Blood flow to the liver and spleen during endotoxin shock in the baboon. Surgery. 1972 Sep;72(3):388–394. [PubMed] [Google Scholar]
  34. Tracey K. J., Lowry S. F. The role of cytokine mediators in septic shock. Adv Surg. 1990;23:21–56. [PubMed] [Google Scholar]
  35. Tracey K. J. Tumor necrosis factor (cachectin) in the biology of septic shock syndrome. Circ Shock. 1991 Oct;35(2):123–128. [PubMed] [Google Scholar]
  36. ZWEIFACH B. W., BENACERRAF B., THOMAS L. The relationship between the vascular manifestations of shock produced by endotoxin, trauma, and hemorrhage. II. The possible role of the reticulo-endothelial system in resistance to each type of shock. J Exp Med. 1957 Sep 1;106(3):403–414. doi: 10.1084/jem.106.3.403. [DOI] [PMC free article] [PubMed] [Google Scholar]

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