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. 1993 Aug;61(8):3240–3243. doi: 10.1128/iai.61.8.3240-3243.1993

The genes encoding the secreted aspartyl proteinases of Candida albicans constitute a family with at least three members.

B B Magee 1, B Hube 1, R J Wright 1, P J Sullivan 1, P T Magee 1
PMCID: PMC280994  PMID: 8335356

Abstract

The secreted aspartyl proteinase activity from Candida albicans is thought to be a potential virulence factor. Four laboratories have cloned a gene from C. albicans encoding this enzyme. When two of these genes sharing 77% homology at the DNA level are hybridized under conditions of high stringency to contour-clamped homogeneous electric field chromosome separations of four different strains, they label different chromosomes: chromosome 6 for SAP1 and chromosome R for SAP2. The existence of different genes for the two sequences was confirmed by polymerase chain reaction. Genomic Southern blots probed with the genes and washed at low stringency revealed several cross-hybridizing bands. Contour-clamped homogeneous electric field chromosome separations probed at low stringency indicated that there was a cross-hybridizing sequence on chromosome 3 in addition to those on chromosomes R and 6. The genes for the secreted aspartyl proteinase activity in C. albicans thus constitute a gene family which we have called the SAP family.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Borg M., Rüchel R. Expression of extracellular acid proteinase by proteolytic Candida spp. during experimental infection of oral mucosa. Infect Immun. 1988 Mar;56(3):626–631. doi: 10.1128/iai.56.3.626-631.1988. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Ganesan K., Banerjee A., Datta A. Molecular cloning of the secretory acid proteinase gene from Candida albicans and its use as a species-specific probe. Infect Immun. 1991 Sep;59(9):2972–2977. doi: 10.1128/iai.59.9.2972-2977.1991. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Goshorn A. K., Scherer S. Genetic analysis of prototrophic natural variants of Candida albicans. Genetics. 1989 Dec;123(4):667–673. doi: 10.1093/genetics/123.4.667. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Hube B., Turver C. J., Odds F. C., Eiffert H., Boulnois G. J., Köchel H., Rüchel R. Sequence of the Candida albicans gene encoding the secretory aspartate proteinase. J Med Vet Mycol. 1991;29(2):129–132. [PubMed] [Google Scholar]
  5. Kwon-Chung K. J., Lehman D., Good C., Magee P. T. Genetic evidence for role of extracellular proteinase in virulence of Candida albicans. Infect Immun. 1985 Sep;49(3):571–575. doi: 10.1128/iai.49.3.571-575.1985. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Lee S. Y., Rasheed S. A simple procedure for maximum yield of high-quality plasmid DNA. Biotechniques. 1990 Dec;9(6):676–679. [PubMed] [Google Scholar]
  7. Lott T. J., Page L. S., Boiron P., Benson J., Reiss E. Nucleotide sequence of the Candida albicans aspartyl proteinase gene. Nucleic Acids Res. 1989 Feb 25;17(4):1779–1779. doi: 10.1093/nar/17.4.1779. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Macdonald F., Odds F. C. Virulence for mice of a proteinase-secreting strain of Candida albicans and a proteinase-deficient mutant. J Gen Microbiol. 1983 Feb;129(2):431–438. doi: 10.1099/00221287-129-2-431. [DOI] [PubMed] [Google Scholar]
  9. Magee B. B., D'Souza T. M., Magee P. T. Strain and species identification by restriction fragment length polymorphisms in the ribosomal DNA repeat of Candida species. J Bacteriol. 1987 Apr;169(4):1639–1643. doi: 10.1128/jb.169.4.1639-1643.1987. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Morrow B., Srikantha T., Soll D. R. Transcription of the gene for a pepsinogen, PEP1, is regulated by white-opaque switching in Candida albicans. Mol Cell Biol. 1992 Jul;12(7):2997–3005. doi: 10.1128/mcb.12.7.2997. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Mukai H., Takeda O., Asada K., Kato I., Murayama S. Y., Yamaguchi H. cDNA cloning of an aspartic proteinase secreted by Candida albicans. Microbiol Immunol. 1992;36(11):1207–1216. doi: 10.1111/j.1348-0421.1992.tb02124.x. [DOI] [PubMed] [Google Scholar]
  12. Rikkerink E. H., Magee B. B., Magee P. T. Opaque-white phenotype transition: a programmed morphological transition in Candida albicans. J Bacteriol. 1988 Feb;170(2):895–899. doi: 10.1128/jb.170.2.895-899.1988. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Ross I. K., De Bernardis F., Emerson G. W., Cassone A., Sullivan P. A. The secreted aspartate proteinase of Candida albicans: physiology of secretion and virulence of a proteinase-deficient mutant. J Gen Microbiol. 1990 Apr;136(4):687–694. doi: 10.1099/00221287-136-4-687. [DOI] [PubMed] [Google Scholar]
  14. Rüchel R. Properties of a purified proteinase from the yeast Candida albicans. Biochim Biophys Acta. 1981 May 14;659(1):99–113. doi: 10.1016/0005-2744(81)90274-6. [DOI] [PubMed] [Google Scholar]
  15. Saiki R. K., Gelfand D. H., Stoffel S., Scharf S. J., Higuchi R., Horn G. T., Mullis K. B., Erlich H. A. Primer-directed enzymatic amplification of DNA with a thermostable DNA polymerase. Science. 1988 Jan 29;239(4839):487–491. doi: 10.1126/science.2448875. [DOI] [PubMed] [Google Scholar]
  16. Scherer S., Magee P. T. Genetics of Candida albicans. Microbiol Rev. 1990 Sep;54(3):226–241. doi: 10.1128/mr.54.3.226-241.1990. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Slutsky B., Buffo J., Soll D. R. High-frequency switching of colony morphology in Candida albicans. Science. 1985 Nov 8;230(4726):666–669. doi: 10.1126/science.3901258. [DOI] [PubMed] [Google Scholar]
  18. Slutsky B., Staebell M., Anderson J., Risen L., Pfaller M., Soll D. R. "White-opaque transition": a second high-frequency switching system in Candida albicans. J Bacteriol. 1987 Jan;169(1):189–197. doi: 10.1128/jb.169.1.189-197.1987. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Staib F. Proteolysis and pathogenicity of Candida albicans strains. Mycopathol Mycol Appl. 1969 May 28;37(4):345–348. doi: 10.1007/BF02129881. [DOI] [PubMed] [Google Scholar]
  20. Togni G., Sanglard D., Falchetto R., Monod M. Isolation and nucleotide sequence of the extracellular acid protease gene (ACP) from the yeast Candida tropicalis. FEBS Lett. 1991 Jul 29;286(1-2):181–185. doi: 10.1016/0014-5793(91)80969-a. [DOI] [PubMed] [Google Scholar]
  21. Wickes B., Staudinger J., Magee B. B., Kwon-Chung K. J., Magee P. T., Scherer S. Physical and genetic mapping of Candida albicans: several genes previously assigned to chromosome 1 map to chromosome R, the rDNA-containing linkage group. Infect Immun. 1991 Jul;59(7):2480–2484. doi: 10.1128/iai.59.7.2480-2484.1991. [DOI] [PMC free article] [PubMed] [Google Scholar]
  22. Wright R. J., Carne A., Hieber A. D., Lamont I. L., Emerson G. W., Sullivan P. A. A second gene for a secreted aspartate proteinase in Candida albicans. J Bacteriol. 1992 Dec;174(23):7848–7853. doi: 10.1128/jb.174.23.7848-7853.1992. [DOI] [PMC free article] [PubMed] [Google Scholar]

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