Skip to main content
PMC home page
Infection and Immunity logoLink to Infection and Immunity
. 1993 Aug;61(8):3533–3535. doi: 10.1128/iai.61.8.3533-3535.1993

Changes in complement and immunoglobulin G receptor expression on neutrophils associated with Porphyromonas gingivalis-induced inhibition of phagocytosis.

H Tai 1, T Kobayashi 1, K Hara 1
PMCID: PMC281034  PMID: 8392973

Abstract

We examined the phagocytic capacity and receptor expression on neutrophils stimulated with Porphyromonas gingivalis soluble products. Stimulated neutrophils had decreased phagocytic capacities and altered expression of CR1, CR3, Fc gamma RII, and Fc gamma RIII. For cases in which TLCK (N-alpha-p-tosyl-L-lysine chloromethyl ketone) neutralized the effects of the stimuli, the P. gingivalis-derived factors causing the phenomena seem to be trypsin-like proteases.

Full text

PDF
3533

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Berger M., O'Shea J., Cross A. S., Folks T. M., Chused T. M., Brown E. J., Frank M. M. Human neutrophils increase expression of C3bi as well as C3b receptors upon activation. J Clin Invest. 1984 Nov;74(5):1566–1571. doi: 10.1172/JCI111572. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Brunkhorst B. A., Strohmeier G., Lazzari K., Weil G., Melnick D., Fleit H. B., Simons E. R. Differential roles of Fc gamma RII and Fc gamma RIII in immune complex stimulation of human neutrophils. J Biol Chem. 1992 Oct 15;267(29):20659–20666. [PubMed] [Google Scholar]
  3. Cutler C. W., Kalmar J. R., Arnold R. R. Phagocytosis of virulent Porphyromonas gingivalis by human polymorphonuclear leukocytes requires specific immunoglobulin G. Infect Immun. 1991 Jun;59(6):2097–2104. doi: 10.1128/iai.59.6.2097-2104.1991. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Dunn P. A., Tyrer H. W. Quantitation of neutrophil phagocytosis, using fluorescent latex beads. Correlation of microscopy and flow cytometry. J Lab Clin Med. 1981 Sep;98(3):374–381. [PubMed] [Google Scholar]
  5. Gordon D. L., Johnson G. M., Hostetter M. K. Ligand-receptor interactions in the phagocytosis of virulent Streptococcus pneumoniae by polymorphonuclear leukocytes. J Infect Dis. 1986 Oct;154(4):619–626. doi: 10.1093/infdis/154.4.619. [DOI] [PubMed] [Google Scholar]
  6. Graziano R. F., Fanger M. W. Fc gamma RI and Fc gamma RII on monocytes and granulocytes are cytotoxic trigger molecules for tumor cells. J Immunol. 1987 Nov 15;139(10):3536–3541. [PubMed] [Google Scholar]
  7. Grenier D., Chao G., McBride B. C. Characterization of sodium dodecyl sulfate-stable Bacteroides gingivalis proteases by polyacrylamide gel electrophoresis. Infect Immun. 1989 Jan;57(1):95–99. doi: 10.1128/iai.57.1.95-99.1989. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Huizinga T. W., Roos D., von dem Borne A. E. Neutrophil Fc-gamma receptors: a two-way bridge in the immune system. Blood. 1990 Mar 15;75(6):1211–1214. [PubMed] [Google Scholar]
  9. Huizinga T. W., van der Schoot C. E., Jost C., Klaassen R., Kleijer M., von dem Borne A. E., Roos D., Tetteroo P. A. The PI-linked receptor FcRIII is released on stimulation of neutrophils. Nature. 1988 Jun 16;333(6174):667–669. doi: 10.1038/333667a0. [DOI] [PubMed] [Google Scholar]
  10. Indik Z., Kelly C., Chien P., Levinson A. I., Schreiber A. D. Human Fc gamma RII, in the absence of other Fc gamma receptors, mediates a phagocytic signal. J Clin Invest. 1991 Nov;88(5):1766–1771. doi: 10.1172/JCI115496. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Kilian M. Degradation of immunoglobulins A2, A2, and G by suspected principal periodontal pathogens. Infect Immun. 1981 Dec;34(3):757–765. doi: 10.1128/iai.34.3.757-765.1981. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Kuijpers T. W., Tool A. T., van der Schoot C. E., Ginsel L. A., Onderwater J. J., Roos D., Verhoeven A. J. Membrane surface antigen expression on neutrophils: a reappraisal of the use of surface markers for neutrophil activation. Blood. 1991 Aug 15;78(4):1105–1111. [PubMed] [Google Scholar]
  13. Kushner B. H., Cheung N. K. Absolute requirement of CD11/CD18 adhesion molecules, FcRII and the phosphatidylinositol-linked FcRIII for monoclonal antibody-mediated neutrophil antihuman tumor cytotoxicity. Blood. 1992 Mar 15;79(6):1484–1490. [PubMed] [Google Scholar]
  14. LOWRY O. H., ROSEBROUGH N. J., FARR A. L., RANDALL R. J. Protein measurement with the Folin phenol reagent. J Biol Chem. 1951 Nov;193(1):265–275. [PubMed] [Google Scholar]
  15. Leino L., Lilius E. M. The up- and down-modulation of immunoglobulin G Fc receptors and complement receptors on activated human neutrophils depends on the nature of activator. J Leukoc Biol. 1992 Feb;51(2):157–163. doi: 10.1002/jlb.51.2.157. [DOI] [PubMed] [Google Scholar]
  16. O'Shea J. J., Brown E. J., Seligmann B. E., Metcalf J. A., Frank M. M., Gallin J. I. Evidence for distinct intracellular pools of receptors for C3b and C3bi in human neutrophils. J Immunol. 1985 Apr;134(4):2580–2587. [PubMed] [Google Scholar]
  17. Schenkein H. A. Failure of Bacteroides gingivalis W83 to accumulate bound C3 following opsonization with serum. J Periodontal Res. 1989 Jan;24(1):20–27. doi: 10.1111/j.1600-0765.1989.tb00853.x. [DOI] [PubMed] [Google Scholar]
  18. Selvaraj P., Rosse W. F., Silber R., Springer T. A. The major Fc receptor in blood has a phosphatidylinositol anchor and is deficient in paroxysmal nocturnal haemoglobinuria. Nature. 1988 Jun 9;333(6173):565–567. doi: 10.1038/333565a0. [DOI] [PubMed] [Google Scholar]
  19. Steinkamp J. A., Wilson J. S., Saunders G. C., Stewart C. C. Phagocytosis: flow cytometric quantitation with fluorescent microspheres. Science. 1982 Jan 1;215(4528):64–66. doi: 10.1126/science.7053559. [DOI] [PubMed] [Google Scholar]
  20. Tsutsui H., Kinouchi T., Wakano Y., Ohnishi Y. Purification and characterization of a protease from Bacteroides gingivalis 381. Infect Immun. 1987 Feb;55(2):420–427. doi: 10.1128/iai.55.2.420-427.1987. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. Van Dyke T. E., Bartholomew E., Genco R. J., Slots J., Levine M. J. Inhibition of neutrophil chemotaxis by soluble bacterial products. J Periodontol. 1982 Aug;53(8):502–508. doi: 10.1902/jop.1982.53.8.502. [DOI] [PubMed] [Google Scholar]
  22. Van Dyke T. E., Horoszewicz H. U., Cianciola L. J., Genco R. J. Neutrophil chemotaxis dysfunction in human periodontitis. Infect Immun. 1980 Jan;27(1):124–132. doi: 10.1128/iai.27.1.124-132.1980. [DOI] [PMC free article] [PubMed] [Google Scholar]
  23. Van Dyke T. E. Neutrophil receptor modulation in the pathogenesis of periodontal diseases. J Dent Res. 1984 Mar;63(3):452–454. doi: 10.1177/00220345840630031701. [DOI] [PubMed] [Google Scholar]
  24. Wingrove J. A., DiScipio R. G., Chen Z., Potempa J., Travis J., Hugli T. E. Activation of complement components C3 and C5 by a cysteine proteinase (gingipain-1) from Porphyromonas (Bacteroides) gingivalis. J Biol Chem. 1992 Sep 15;267(26):18902–18907. [PubMed] [Google Scholar]
  25. Yoneda M., Maeda K., Aono M. Suppression of bactericidal activity of human polymorphonuclear leukocytes by Bacteroides gingivalis. Infect Immun. 1990 Feb;58(2):406–411. doi: 10.1128/iai.58.2.406-411.1990. [DOI] [PMC free article] [PubMed] [Google Scholar]
  26. Yoshimura F., Nishikata M., Suzuki T., Hoover C. I., Newbrun E. Characterization of a trypsin-like protease from the bacterium Bacteroides gingivalis isolated from human dental plaque. Arch Oral Biol. 1984;29(7):559–564. doi: 10.1016/0003-9969(84)90078-5. [DOI] [PubMed] [Google Scholar]

Articles from Infection and Immunity are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES