Skip to main content
NCBI home page
Infection and Immunity logoLink to Infection and Immunity
. 1993 Aug;61(8):3562–3564. doi: 10.1128/iai.61.8.3562-3564.1993

Role of Porphyromonas gingivalis-derived fibroblast-activating factor in bone resorption.

J Mihara 1, T Yoneda 1, S C Holt 1
PMCID: PMC281042  PMID: 8392974

Abstract

A 24-kDa protein was isolated from the outer membrane vesicles of Porphyromaonas gingivalis W50. This protein, referred to as fibroblast-activating factor (FAF), was examined for its bone-resorptive ability by the rat long-bone assay and the mouse bone marrow cell culture system. FAF resulted in a significant release of 45Ca from cultured bones, as well as the formation of tartrate-resistant acid phosphatase-positive monocytes. These cultures were compared with control cells. FAF therefore might be considered a significant bacterially expressed protein which could affect and modulate the resorption or destruction of tissue and alveolar bone in the local periodontal environment.

Full text

PDF
3562

Images in this article

3563Image
on p.3563

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Dewhirst F. E. N-acetyl muramyl dipeptide stimulation of bone resorption in tissue culture. Infect Immun. 1982 Jan;35(1):133–137. doi: 10.1128/iai.35.1.133-137.1982. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Dewhirst F. E., Stashenko P. P., Mole J. E., Tsurumachi T. Purification and partial sequence of human osteoclast-activating factor: identity with interleukin 1 beta. J Immunol. 1985 Oct;135(4):2562–2568. [PubMed] [Google Scholar]
  3. Hausmann E., Raisz L. G., Miller W. A. Endotoxin: stimulation of bone resorption in tissue culture. Science. 1970 May 15;168(3933):862–864. doi: 10.1126/science.168.3933.862. [DOI] [PubMed] [Google Scholar]
  4. Holt S. C., Bramanti T. E. Factors in virulence expression and their role in periodontal disease pathogenesis. Crit Rev Oral Biol Med. 1991;2(2):177–281. doi: 10.1177/10454411910020020301. [DOI] [PubMed] [Google Scholar]
  5. Iino Y., Hopps R. M. The bone-resorbing activities in tissue culture of lipopolysaccharides from the bacteria Actinobacillus actinomycetemcomitans, Bacteroides gingivalis and Capnocytophaga ochracea isolated from human mouths. Arch Oral Biol. 1984;29(1):59–63. doi: 10.1016/0003-9969(84)90043-8. [DOI] [PubMed] [Google Scholar]
  6. Mihara J., Holt S. C. Purification and characterization of fibroblast-activating factor isolated from Porphyromonas gingivalis W50. Infect Immun. 1993 Feb;61(2):588–595. doi: 10.1128/iai.61.2.588-595.1993. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Mihara J., Miyazawa Y., Holt S. C. Modulation of growth and function of human gingival fibroblasts by fibroblast-activating factor derived from Porphyromonas gingivalis W50. Infect Immun. 1993 Feb;61(2):596–601. doi: 10.1128/iai.61.2.596-601.1993. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Millar S. J., Goldstein E. G., Levine M. J., Hausmann E. Lipoprotein: a gram-negative cell wall component that stimulates bone resorption. J Periodontal Res. 1986 May;21(3):256–259. doi: 10.1111/j.1600-0765.1986.tb01457.x. [DOI] [PubMed] [Google Scholar]
  9. Millar S. J., Goldstein E. G., Levine M. J., Hausmann E. Modulation of bone metabolism by two chemically distinct lipopolysaccharide fractions from Bacteroides gingivalis. Infect Immun. 1986 Jan;51(1):302–306. doi: 10.1128/iai.51.1.302-306.1986. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Pashley D. H., Livingston M. J., Whitford G. M. The effect of molecular size on reflection coefficients in human dentine. Arch Oral Biol. 1979;24(6):455–460. doi: 10.1016/0003-9969(79)90008-6. [DOI] [PubMed] [Google Scholar]
  11. RAISZ L. G. BONE RESORPTION IN TISSUE CULTURE. FACTORS INFLUENCING THE RESPONSE TO PARATHYROID HORMONE. J Clin Invest. 1965 Jan;44:103–116. doi: 10.1172/JCI105117. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Slots J. Bacterial specificity in adult periodontitis. A summary of recent work. J Clin Periodontol. 1986 Nov;13(10):912–917. doi: 10.1111/j.1600-051x.1986.tb01426.x. [DOI] [PubMed] [Google Scholar]
  13. Slots J. Microflora in the healthy gingival sulcus in man. Scand J Dent Res. 1977 May;85(4):247–254. doi: 10.1111/j.1600-0722.1977.tb00560.x. [DOI] [PubMed] [Google Scholar]
  14. Takahashi N., Yamana H., Yoshiki S., Roodman G. D., Mundy G. R., Jones S. J., Boyde A., Suda T. Osteoclast-like cell formation and its regulation by osteotropic hormones in mouse bone marrow cultures. Endocrinology. 1988 Apr;122(4):1373–1382. doi: 10.1210/endo-122-4-1373. [DOI] [PubMed] [Google Scholar]
  15. Wilson M., Meghji S., Harvey W. Effect of capsular material from Haemophilus actinomycetemcomitans on bone collagen synthesis in vitro. Microbios. 1988;54(220-221):181–185. [PubMed] [Google Scholar]

Articles from Infection and Immunity are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES