Skip to main content
NCBI home page
Infection and Immunity logoLink to Infection and Immunity
. 1993 Aug;61(8):3574–3577. doi: 10.1128/iai.61.8.3574-3577.1993

Electrophoretic karyotype and chromosome assignments for a pathogenic and a nonpathogenic strain of Entamoeba histolytica.

R Petter 1, S Rozenblatt 1, D Schechtman 1, T E Wellems 1, D Mirelman 1
PMCID: PMC281045  PMID: 8335392

Abstract

The electrophoretic karyotypes of a pathogenic and a nonpathogenic strain of Entamoeba histolytica were determined by pulsed-field gel electrophoresis. A number of previously isolated genes were assigned to specific chromosomal bands. Significant differences between the chromosomal patterns of these strains as well as in the assignment of most genes were found.

Full text

PDF
3574

Images in this article

3575Image
on p.3575
3576Image
on p.3576
3576Image
on p.3576
3576Image
on p.3576

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Arhets P., Rahim Z., Raymond-Denise A., Sansonetti P., Guillén N. Identification of a myosin heavy chain gene from Entamoeba histolytica. Arch Med Res. 1992;23(2):41–43. [PubMed] [Google Scholar]
  2. Beverley S. M. Characterization of the 'unusual' mobility of large circular DNAs in pulsed field-gradient electrophoresis. Nucleic Acids Res. 1988 Feb 11;16(3):925–939. doi: 10.1093/nar/16.3.925. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Clark C. G., Diamond L. S. Ribosomal RNA genes of 'pathogenic' and 'nonpathogenic' Entamoeba histolytica are distinct. Mol Biochem Parasitol. 1991 Dec;49(2):297–302. doi: 10.1016/0166-6851(91)90073-f. [DOI] [PubMed] [Google Scholar]
  4. Clark C. G., Lai E. Y., Fulton C., Cross G. A. Electrophoretic karyotype and linkage groups of the amoeboflagellate Naegleria gruberi. J Protozool. 1990 Sep-Oct;37(5):400–408. doi: 10.1111/j.1550-7408.1990.tb01164.x. [DOI] [PubMed] [Google Scholar]
  5. Diamond L. S., Harlow D. R., Cunnick C. C. A new medium for the axenic cultivation of Entamoeba histolytica and other Entamoeba. Trans R Soc Trop Med Hyg. 1978;72(4):431–432. doi: 10.1016/0035-9203(78)90144-x. [DOI] [PubMed] [Google Scholar]
  6. Edman U., Meza I., Agabian N. Genomic and cDNA actin sequences from a virulent strain of Entamoeba histolytica. Proc Natl Acad Sci U S A. 1987 May;84(9):3024–3028. doi: 10.1073/pnas.84.9.3024. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Feinberg A. P., Vogelstein B. "A technique for radiolabeling DNA restriction endonuclease fragments to high specific activity". Addendum. Anal Biochem. 1984 Feb;137(1):266–267. doi: 10.1016/0003-2697(84)90381-6. [DOI] [PubMed] [Google Scholar]
  8. Feinberg A. P., Vogelstein B. A technique for radiolabeling DNA restriction endonuclease fragments to high specific activity. Anal Biochem. 1983 Jul 1;132(1):6–13. doi: 10.1016/0003-2697(83)90418-9. [DOI] [PubMed] [Google Scholar]
  9. Foote S. J., Kemp D. J. Chromosomes of malaria parasites. Trends Genet. 1989 Oct;5(10):337–342. doi: 10.1016/0168-9525(89)90139-x. [DOI] [PubMed] [Google Scholar]
  10. Garfinkel L. I., Giladi M., Huber M., Gitler C., Mirelman D., Revel M., Rozenblatt S. DNA probes specific for Entamoeba histolytica possessing pathogenic and nonpathogenic zymodemes. Infect Immun. 1989 Mar;57(3):926–931. doi: 10.1128/iai.57.3.926-931.1989. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Gottesdiener K., Garciá-Anoveros J., Lee M. G., Van der Ploeg L. H. Chromosome organization of the protozoan Trypanosoma brucei. Mol Cell Biol. 1990 Nov;10(11):6079–6083. doi: 10.1128/mcb.10.11.6079. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Grodberg J., Salazar N., Oren R., Mirelman D. Autonomous replication sequences in an extrachromosomal element of a pathogenic Entamoeba histolytica. Nucleic Acids Res. 1990 Sep 25;18(18):5515–5519. doi: 10.1093/nar/18.18.5515. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Hightower R. C., Santi D. V. Migration properties of circular DNAs using orthogonal-field-alternation gel electrophoresis. Electrophoresis. 1989 May-Jun;10(5-6):283–290. doi: 10.1002/elps.1150100503. [DOI] [PubMed] [Google Scholar]
  14. Huber M., Garfinkel L., Gitler C., Mirelman D., Revel M., Rozenblatt S. Entamoeba histolytica: cloning and characterization of actin cDNA. Mol Biochem Parasitol. 1987 Jul;24(3):227–235. doi: 10.1016/0166-6851(87)90154-x. [DOI] [PubMed] [Google Scholar]
  15. Huber M., Garfinkel L., Gitler C., Mirelman D., Revel M., Rozenblatt S. Nucleotide sequence analysis of an Entamoeba histolytica ferredoxin gene. Mol Biochem Parasitol. 1988 Oct;31(1):27–33. doi: 10.1016/0166-6851(88)90142-9. [DOI] [PubMed] [Google Scholar]
  16. Huber M., Koller B., Gitler C., Mirelman D., Revel M., Rozenblatt S., Garfinkel L. Entamoeba histolytica ribosomal RNA genes are carried on palindromic circular DNA molecules. Mol Biochem Parasitol. 1989 Jan 15;32(2-3):285–296. doi: 10.1016/0166-6851(89)90077-7. [DOI] [PubMed] [Google Scholar]
  17. Korman S. H., Le Blancq S. M., Deckelbaum R. J., Van der Ploeg L. H. Investigation of human giardiasis by karyotype analysis. J Clin Invest. 1992 Jun;89(6):1725–1733. doi: 10.1172/JCI115774. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Lighthall G. K., Giannini S. H. The chromosomes of Leishmania. Parasitol Today. 1992 Jun;8(6):192–199. doi: 10.1016/0169-4758(92)90263-2. [DOI] [PubMed] [Google Scholar]
  19. Mackenstedt U., Schmidt M., Raether W., Mehlhorn H., Uphoff M. Increase of DNA content in tissue stages of Entamoeba histolytica strain SFL 3. Parasitol Res. 1990;76(5):373–378. doi: 10.1007/BF00933543. [DOI] [PubMed] [Google Scholar]
  20. Mann B. J., Torian B. E., Vedvick T. S., Petri W. A., Jr Sequence of a cysteine-rich galactose-specific lectin of Entamoeba histolytica. Proc Natl Acad Sci U S A. 1991 Apr 15;88(8):3248–3252. doi: 10.1073/pnas.88.8.3248. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. McKerrow J. H. Pathogenesis in amebiasis: is it genetic or acquired? Infect Agents Dis. 1992 Feb;1(1):11–14. [PubMed] [Google Scholar]
  22. Mirelman D., Bracha R., Wexler A., Chayen A. Changes in isoenzyme patterns of a cloned culture of nonpathogenic Entamoeba histolytica during axenization. Infect Immun. 1986 Dec;54(3):827–832. doi: 10.1128/iai.54.3.827-832.1986. [DOI] [PMC free article] [PubMed] [Google Scholar]
  23. Mirelman D., Keren Z., Bracha R. Cloning and partial characterization of an antigen detected on membrane surfaces of non-pathogenic strains of Entamoeba histolytica. Arch Med Res. 1992;23(2):49–53. [PubMed] [Google Scholar]
  24. Mirelman D. Pathogenic versus nonpathogenic Entamoeba histolytica. Infect Agents Dis. 1992 Feb;1(1):15–18. [PubMed] [Google Scholar]
  25. Orozco E. Pathogenesis in amebiasis. Infect Agents Dis. 1992 Feb;1(1):19–21. [PubMed] [Google Scholar]
  26. Petter R., Rozenblatt S., Nuchamowitz Y., Mirelman D. Linkage between actin and ribosomal protein L21 genes in Entamoeba histolytica. Mol Biochem Parasitol. 1992 Dec;56(2):329–333. doi: 10.1016/0166-6851(92)90182-j. [DOI] [PubMed] [Google Scholar]
  27. Rimm D. L., Pollard T. D., Hieter P. Resolution of Acanthamoeba castellanii chromosomes by pulsed field gel electrophoresis and construction of the initial linkage map. Chromosoma. 1988 Nov;97(3):219–223. doi: 10.1007/BF00292964. [DOI] [PubMed] [Google Scholar]
  28. Rovai L., Tripp C., Stuart K., Simpson L. Recurrent polymorphisms in small chromosomes of Leishmania tarentolae after nutrient stress or subcloning. Mol Biochem Parasitol. 1992 Jan;50(1):115–125. doi: 10.1016/0166-6851(92)90249-j. [DOI] [PubMed] [Google Scholar]
  29. Sargeaunt P. G., Williams J. E., Grene J. D. The differentiation of invasive and non-invasive Entamoeba histolytica by isoenzyme electrophoresis. Trans R Soc Trop Med Hyg. 1978;72(5):519–521. doi: 10.1016/0035-9203(78)90174-8. [DOI] [PubMed] [Google Scholar]
  30. Sibley L. D., Boothroyd J. C. Construction of a molecular karyotype for Toxoplasma gondii. Mol Biochem Parasitol. 1992 Apr;51(2):291–300. doi: 10.1016/0166-6851(92)90079-y. [DOI] [PubMed] [Google Scholar]
  31. Spice W. M., Ackers J. P. The amoeba enigma. Parasitol Today. 1992 Dec;8(12):402–406. doi: 10.1016/0169-4758(92)90188-8. [DOI] [PubMed] [Google Scholar]
  32. Tannich E. Pathogenesis in amebiasis. Infect Agents Dis. 1992 Feb;1(1):22–23. [PubMed] [Google Scholar]
  33. Triglia T., Wellems T. E., Kemp D. J. Towards a high-resolution map of the Plasmodium falciparum genome. Parasitol Today. 1992 Jul;8(7):225–229. doi: 10.1016/0169-4758(92)90118-l. [DOI] [PubMed] [Google Scholar]
  34. Valdés J., de la Cruz Hernández F., Ocádiz R., Orozco E. Molecular karyotype of Entamoeba histolytica and Entamoeba invadens. Trans R Soc Trop Med Hyg. 1990 Jul-Aug;84(4):537–541. doi: 10.1016/0035-9203(90)90032-a. [DOI] [PubMed] [Google Scholar]
  35. Van der Ploeg L. H., Smits M., Ponnudurai T., Vermeulen A., Meuwissen J. H., Langsley G. Chromosome-sized DNA molecules of Plasmodium falciparum. Science. 1985 Aug 16;229(4714):658–661. doi: 10.1126/science.3895435. [DOI] [PubMed] [Google Scholar]

Articles from Infection and Immunity are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES